Biochemical characterization of Pakistani Zea mays landraces growing in the remote areas of Khyber Pakhtunkhwa
Paper Details
Biochemical characterization of Pakistani Zea mays landraces growing in the remote areas of Khyber Pakhtunkhwa
Abstract
The landraces extensity cultivated in Malak and Division of Pakistan hold important adaptations to local environmental condition, being express significant level of genetic diversity. In the present investigation, The Genetic divergence in 29 zea maize landraces was assessed using seed storage protein through SDS-PAGE. In total, eight loci were express which were entirely polymorphic and high level of genetic diversity was observed in L-8 i.e.10.34% followed by L-2 with 17.2% polymorphism. Among landraces, ST-2 was unique because of the expression of all loci. Genetic tree based on protein binary data matrix delineated landraces into two main groups. It was found that high level of divergence exist within and between collection sites. In dendrogram, each group represented landraces of different collection sites, but group-1 was the diverse group and represented high level of genetic diversity within landraces reported from Swat, Bajaur, Dir and Buner.In Group-2 majority of the landraces showing tight linkage, although originated from different agro ecological zones like Malakand, Buner and Dir. This may be due to the utilization of shared gene pool at different agro ecological zones. Significant level of genetic diversity existed within landraces will decrease the genetic vulnerability and will help in the selection of superior genotypes for future breeding programs.
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