TITLE: Efficient plant regeneration from cotyledonary explants of Tunisian Cucumis melo L. cv. Maazoun and Beji
By: Rhimi Awatef, Boussaid Mohamed
Key Words: Buds; caulogenesis; Cucumis melo L. cv. Maazoun and Beji; plant regeneration; tissue culture.
J. Bio. Env. Sci. 8(2), 166-173, February 2016.
A procedure for the regeneration of melon (Cucumis melo L.) cv. Maazoun and Beji via shoot organogenesis from cotyledon explants is described. Cotyledons explants cultivated on the basal Murashige and Skoog (1962) medium, added with combinations of BAP, NAA and 2,4-D developed adventitious shoots after two weeks. The rates of caulogenesis varied according to the medium hormonal composition and the genotype. The best induction medium for a morphogenic response was MS containing 0.5 mgl-1 NAA and 2 mgl-1 BAP. The highest caulogenesis percentages were 79.16% and 58.33% for Maazoun and Beji cultivars respectively. The mean number of buds per callus was 2.92.
Development phases were characterized by histological studies. Some phases of shoot organogenesis were observed in Cucumis melo L. Maazoun and Beji cultivars.The shoots were rooted in MS medium without growth regulators or added NAA (0.5 or 1 mgl-1). The highest bud rooting of buds occurred on MS medium added with 1 mg/l NAA. The percentage of rooting varied from 16.66 to 75%. The highest percentage of survival plants after acclimatization were 41.73 and 57.63 for Beji and Maazoun respectively