Proteome analysis of wheat (Triticum aestivum L.) root under sodium chloride stress
By: Mahdi Akbari, Mahmoud Toorchi
Key Words: NaCl stress, root proteome, two-dimensional electrophoresis, wheat.
J. Bio. Env. Sci. 8(4), 209-220, April 2016.
Salinity is the most important abiotic stresses adversely affect the quality and quantity of crops, in which 20% of the world’s irrigated agricultural lands are affected by salinity. Wheat is an oldest and the first crop used for bread making for human nutrition. To investigate the response of wheat root to NaCl stress, a susceptible, Arta, and resistance, Bam, wheat varieties were grown under both non-stress and stress conditions. Stress plants were exposed to 250 mM of NaCl based on a completely randomized design with four replications. Proteins were extracted from root and proteomic analysis was performed by two-dimensional polyacrylamide gel electrophoresis. Proteome profile of root in susceptible variety lead to the identification of 21 differentially changed protein spots compare with 49 spots of tolerant variety. The regulatory changed proteins belongs to stress-responsive proteins and others related processes including metabolism and energy; scavenging of reactive oxygen species and detoxifying; protein translation, processing and degradation; cell wall-related proteins; Amino acid metabolism and hormone-related proteins; signal transduction network; cytoskeleton; transcription-related proteins.