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Prokaryotic Community Profiles of Soils from Mayon Volcano, Philippines Based on 16S Ribosomal RNA Gene Sequences

By: Kristel Mae DL. Perdigon, Asuncion K. Raymundo, Rina B. Opulencia

Key Words: 16S rRNA gene, Bacteria, Archaea, Mayon Volcano, Diversity.

J. Bio. Env. Sci. 8(4), 221-230, April 2016.

Abstract

Mayon Volcano is the Philippines’ most active volcano. Despite extensive pedological, ecological and ethnobotanical studies, no published information is known about its soil microflora. In this study, to determine the microbial community profiles, 16S rRNA gene was amplified and sequenced from genomic DNA isolated from volcanic soils collected from altitudinal gradients of Mayon Volcano. Phylogenetic analyses revealed 10 bacterial phyla, including an unclassified group, with Acidobacteria (40.6%) as the most dominant phylum. Archaea were distributed into three phyla and an unclassified archaeal group (53.9%), which comprised the majority. The composition of the prokaryotic community suggests roles in the cycling of organic and inorganic nutrients in Mayon Volcano ecosystem. At p<0.1, soil pH and organic matter content showed significant correlation with species richness of Archaea and diversity of Bacteria. In contrast, altitude, soil temperature and soil moisture content showed no significant influence on the composition and distribution of microorganisms in Mayon Volcano. This study provides the first known information on the prokaryotic composition of Mayon Volcano, including the soil properties that influence the structuring of these communities.

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