International network for natural sciences – research journal
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Prevalence of Eimeria bovis in cattles of Cholistan desert, Pakistan

By: Syeda Rida Zainab, Mirza Imran Shahzad, Muhammad Zahid Mustafa, Maryam Arshad, Tahira Ruby

Key Words: Fecal samples, Cholistani cattle, Eimeria bovis.

J. Bio. Env. Sci. 9(2), 94-98, August 2016.


Protozoan parasites are responsible for a number of diseases in livestock thus affecting a country economy. The coccidiosis is very common parasitic disease in livestock of Pakistan especially in desert animals. A valid procedure was used to detect E. bovisin fecal samples of cholistanicow. PCR reaction was performed using species specific primer for amplification of ITS-1 region of rRNA gene. E. bovis was confirmed in fecal samples obtained cholistani cow while E. zuernii was absent. The PCR based detection of Eimeria parasites seemed highly sensitive when compared with traditional morphological examination of oocytes.

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