International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Phytase supplementation improves growth performance and crude protein digestibility in Labeo rohita fingerlings fed barley meal based diet

By: Mahroze Fatima, Muhammad Afzal, Syed Zakir Hussain Shah, Syed Makhdoom Hussain

Key Words: Growth, Graded phytase levels, Labeo rohita, Nutrients digestibility

J. Bio. Env. Sci. 10(6), 124-132, June 2017.

Abstract

Barley is a staple cereal grain, full of digestible proteins and energy, can be effectively used to replace fishmeal. Phytase is an enzyme used to hydrolyze phytate (anti-nutrient) present in plant proteins. Therefore, this study was designed to evaluate the optimum level of phytase supplementation for maximum growth and nutrient digestibility in Labeo rohita fingerlings fed barley meal based diet. Fish (average body weight 14.28±0.14 g) were fed on 8 experimental diets including 1 reference and 7 test diets. Reference diet was used as standard diet and formulated to provide all necessary nutrients required for normal fish growth. Test diets were consisted of 70% reference and 30% barley meal and were supplemented with graded levels of phytase (0, 250, 500, 750, 1000, 1250, 1500 FTUkg-1). Chromic oxide was used as inert marker to assess nutrient digestibility. Fingerlings having 750 FTU kg-1 phytase supplemented diet showed significantly (p<0.05) improved growth and feed performance as compared to reference and other test diets. Similarly, digestibility data also showed maximum absorption of crude protein and gross energy at 750 FTUkg-1 phytase level. In conclusion, phytase supplementation at the level of 750 FTUkg-1diet can improve growth performance and nutrient digestibility of L. rohita fingerlings to its maximum level in barley meal based diet. However, higher levels of its supplementation were not helpful to further improve the growth and digestibility performance.

| Views 18 |

Phytase supplementation improves growth performance and crude protein digestibility in Labeo rohita fingerlings fed barley meal based diet

Ai Q, Mai K, Zhang W, Xu W, Tan B, Zhang C, Li H. 2007. Effects of exogenous enzymes (phytase, non-starch polysaccharide enzyme) in diets on growth, feed utilization, nitrogen and phosphorus excretion of Japanese seabass, Lateolabrax japonicus. Comparative Biochemistry and Physiology 147, 502- 508.

http://dx.doi.org/10.1016/j.cbpa.2007.01.026.

Alvi AS. 1994. Adventitious toxins in plant origin feedstuffs: Quantification and tolerance level in fish. Masters dissertation, Aligarh Muslim University, Aligarh, India.

AOAC (Association of Official Analytical Chemists). 1995. Official Methods of Analysis. 15th Ed., Association of Official Analytical Chemist, Washington, D.C. USA, p.1094

Baruah, K, Pal AK, Sahu NP, Debnath D. 2007. Microbial phytase supplementation in rohu, Labeo rohita, diets enhances growth performance and nutrient digestibility. Journal of the World Aquaculture Society 38, 129- 137.

http://dx.doi.org/10.1111/j.1749-7345.2006.00081.x

Baruah K, Sahu NP., Pal AK, Debnath D. 2004. Dietary Phytase: An ideal approach for cost effective and low-polluting aquafeed. NAGA, World Fish Center Quarterly 27, 15- 19.

http://www.worldfishcenter.org/Naga/na_2314.pdf.

Cao L, Yang Y, Wang WM, Yakupitiyage A, Yuan DR Diana JS. 2008. Effects of pretreatment with microbial phytase on phosphorous utilization and growth performance of Nile tilapia (Oreochromis niloticus). Aquaculture Nutrition 14, 99- 109.

http://dx.doi.org/10.1111/j.1365-2095.2007.00508.x.

Cheng ZJ, Hardy RW. 2002. Effect of microbial phytase on apparent nutrient digestibility of barley, canola meal, wheat and wheat middlings, measured in vivo using rainbow trout (Oncorhynchus mykiss). Aquaculture Nutrition 8, 271- 277.

http://dx.doi.org/10.1046/j.13652095.2002.00219.x.

Cheryan M, Rackis JJ. 1980. Phytic acid interactions in food systems. Critical Reviews in Food Science and Nutrition 13, 297- 335.

http://dx.doi.org/10.1080/10408398009527293.

Debnath D. 2003. Effect of dietary microbial phytase supplementation on growth performance and body composition of Pangasius pangasius fingerlings. Central institution of fisheries education, versova, Mumbai, India. M.F.Sc. thesis.

Debnath D, Pal AK, Sahu NP, Jain KK, Yengkokpam, Mukherjee SC. 2005. Effect of dietary microbial phytase supplementation on growth and nutrient digestibility of Pangasius pangasius (Hamilton) fingerlings. Aquaculture 36, 180- 187.

http://dx.doi.org/10.1111/j.1365-2109.2004.01203.x.

Divakaran S, Leonard GO, Ian PF. 2002. Note on the methods for determination of chromic oxide in shrimp feeds. Journal of Agriculture and Food Chemistry 50, 464- 467.

Higgs DA, Dosanjh BS, Prendergast AF, Beames R., Hardy RW, Riley W, Deacon G. 1995. Chapter 11. Use of rapeseed/canola protein products in finfish diets. In: Sessa, D, Lim C, Eds. AOCS monograph entitled “Nutrition and utilization technology in aquaculture”. AOCS Press, Champaign, IL, 130- 156 P.

Hardy RW. 1995. Current issues in salmonid nutrition. In: Lim CE et al. Eds. Nutrition and utilization technology in aquaculture. AOCS Press, Champaign, 26- 35 P.

Hussain SM, Afzal M, Javid A, Aslam N, Hussain M, Shah SZH, Azmat H,   Mubarik MS. 2015. Role of phytase supplementation in improving nutrient digestibility in Labeo rohita (Hamilton, 1822) fingerlings fed on cottonseed meal based diet. Indian Journal of Fisheries 62, 78- 84.

Hussain SM, Afzal M, Rana SA, Javid A, Iqbal M. 2011. Effect of phytase supplementation on growth performance and nutrient digestibility of Labeo rohita fingerlings fed on corn gluten meal-based diets. International Journal of Agriculture and Biology 13, 916- 922.

Kumar V, Sinha AK, Makkar HPS, Deboeck G, Becker K. 2011. Phytate and phytase in fish nutrition. Journal of Animal Physiology and Animal Nutrition 3, 335- 364.

http://dx.doi.org/10.1111/j.1439-0396.2011.01169.x.

Li MH, Robinson EH. 1997. Microbial phytase can replace inorganic phosphorous supplements in channel catfish Ictalurus punctatus diets. Journal of the World Aquaculture Society 28, 402- 406.

http://dx.doi.org/10.1111/j.17497345.1997.tb00287.x.

Liener IE. 1994. Implications of antinutritional components in soybean foods. Critical Reviews in Food Science and Nutrition 34, 31- 67.

http://dx.doi.org/10.1080/10408399409527649.

Lei XG, Ku PK, Miller ER, Ullrey DE, Yokoyama MT. 1993. Supplemental microbial phytase improves bioavailability of dietary zinc to weanling pigs. Journal of Nutrition 123, 1117- 1123.

Lim SJ, Lee KJ. 2009. Partial replacement of fish meal by cottonseed meal and soybean meal with iron and phytase supplementation for parrot fish Oplegnathus fasciatus. Aquaculture 290, 283- 289. http://dx.doi.org/10.1016/j.aquaculture.2009.02.018

Mohseni M, Bahmani M, Pourali H, Poudeaghani M, Bae JY, Bai SC. 2009. Effect of soybean meal as a fishmeal replacement dietary lysine and methionine supplementation in great sturgeon Huso huso. In: 6th International Symposium on Sturgeon, Hubei Province, China, 25- 31 P.

NRC (National Research Council). 1993. Nutrient Requirements of Fish. National Academic Press, Washington, DC, 114.

Nwanna LC, Schwarz FJ. 2007. Effect of supplemental phytase on growth, phosphorus digestibility and bone mineralization of common carp (Cyprinus carpio L.). Aquaculture Research 38, 1037- 1044.

http://dx.doi.org/10.1111/j.1365-2109.2007.01752.x.

Papatryphon E, Howell RA, Soares JH. 1999. Growth and mineral absorption by striped bass Morone saxatilis fed a plant feedstuff based diet supplemented with phytase. Journal of the World Aquaculture Society 30, 161- 73.

http://dx.doi.org/10.1111/j.17497345.1999.tb00863.x

Pham MA, Lee KJ, Dang TM, Lim SJ, Ko GY, Eo J, Oh DH. 2008. Improved apparent digestibility coefficient of protein and phosphorus by supplementation of microbial phytase in diets containing cottonseed and soybean meal for juvenile olive flounder (Paralichthys olivaceus). Asian-Australasian Journal of Animal Sciences 21, 1367- 1375.

Portz L, Liebert F. 2004. Growth, nutrient utilization and parameters of mineral metabolism in Nile tilapia, Oreochromis niloticus fed plant based diet with graded levels of microbial phytase. Journal of Animal Physiology and Animal Nutrition 88, 311- 320.

http://dx.doi.org/10.1111/j.1439-0396.2004.00486.x.

Rasmussen SK, Hatzack F. 1998. Identification of two low-phytate barley (Hordeum vulgare L.) grain mutants by TLC and genetic analysis. Hereditas 129, 107-112.

http://dx.doi.org/10.1111/j.1601-5223.1998.00107.x.

Rowland SJ, Ingram BA. 1991. Diseases of Australian native fishes. In: Fisheries Bulletin 4 NSW Fisheries, Sydney, NSW, and Australia.

Sajjadi M, Carter CG. 2004. Effect of phytic acid and phytase on feed intake, growth, digestibility and trypsin activity in Atlantic salmon (Salmo salar L.). Aquaculture Nutrition 10, 135- 142.

http://dx.doi.org/10.1111/j.1365-2095.2003.00290.x.

Sardar P, Randhawa HS, Abid M, Prabhakar SK. 2007. Effect of dietary microbial phytase supplementation on growth performance, nutrient utilization, body compositions and haemato-biochemical profiles of Cyprinus carpio (L.) fingerlings fed soy protein-based diet. Aquaculture Nutrition 13, 444- 456.

http://dx.doi.org/10.1111/j.1365-2095.2007.00497.x.

Sarker PK, Fukada H, Hosokawa H, Masumoto T. 2006. Effect of phytase with inorganic phosphorus supplement diet on nutrient availability of Japanese flounder (Paralichthys olivaceus). Aquaculture Science 54, 391- 398.

http://doi.org/10.11233/aquaculturesci1953.54.391.

Shah SZH, Afzal M, Akmal A, Fatima M, Hussain SM. 2016. Effect of citric acid and phytase on growth performance and mineralization of Labeo rohita juveniles fed soybean meal based diet. International Journal of Agriculture and Biology 18, 111- 116.

http://dx.doi.org/10.17957/IJAB/15.0071.

Shah SZH, Afzal M, Fatima M, Hussain SM, Bhatti MA, Hayat HF. 2015. Supplementation of phytase and citric acid to soybean meal based diet enhance muscle mineralization of rohu, Labeo rohita, juveniles. Journal of Science 5, 796- 800.

Vielma J, M-Kinen T, Ekholm P, Koskela J. 2000. Influence of dietary soy and phytase levels on performance and body composition of large rainbow trout, Oncorhynchus mykiss and algal availability of phosphorus load. Aquaculture 183, 349- 362.

http://dx.doi.org/10.1016/S0044-8486(99)00299-9.

Wang F, Yang Y, Han Z, Dong H, Yang C, Zou Z. 2009. Effects of phytase pretreatment of soybean meal and phytase-sprayed in diets on growth, apparent digestibility coefficient and nutrient excretion of rainbow trout (Oncorhynchus mykiss Walbaum). Aquaculture International 17, 143- 157.

http://dx.doi.org/10.1007/s10499-008-9187-5.

Mahroze Fatima, Muhammad Afzal, Syed Zakir Hussain Shah, Syed Makhdoom Hussain.
Phytase supplementation improves growth performance and crude protein digestibility in Labeo rohita fingerlings fed barley meal based diet.
J. Bio. Env. Sci. 10(6), 124-132, June 2017.
http://www.innspub.net/jbes/phytase-supplementation-improves-growth-performance-crude-protein-digestibility-labeo-rohita-fingerlings-fed-barley-meal-based-diet/
Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
http://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update