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Molecular genotyping of methicillin-resistant Staphylococcus aureus by employing DNA marker technology

By: Naima Mahreen, Aasma Akhtar, Javaria Altaf, Muhammad Saqib, Mian Abdul Hafeez, Faisal Saeed Awan

Key Words: Staphylococcus aureus; Methicillin-resistant; Genetic diversity; RAPD.

J. Bio. Env. Sci. 10(6), 330-335, June 2017.

Abstract

Staphylococcus aureus are Gram positive, non-motile and facultative anaerobic coccus often golden yellow cells with pigments and usually arranged in clusters like grapes. Methicillin-Resistant Staphylococcus aureus (MRSA) are S. aureus strains that are resistant to methicillin and many other antibiotics. The present study aimed to molecular characterizes the 23 MRSA isolates obtained from effected patients and characterized by Randomly Amplified Polymorphic DNA (RAPD). Total of 14 RAPD primers amplified 827 fragments with range of 3 to 8 and level of polymorphism was 93.9%. Genetic similarities range is 88.73% to 52.11%, maximum genetic similarity was found between strain number 143 and 136 while the minimum genetic similarity was found among strain number 141 and 88. Genetic relationship among the MRSA isolates was analyzed through cluster analysis. All isolates were clustered in three major groups and maximum isolates were present in the second group. The present findings could be of beneficial use in scheming the sources as well as modes of transmission, trailing the spread of strains within and between hospitals, and mainly inhibits the nosocomial infections that are caused by the MRSA.

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Molecular genotyping of methicillin-resistant Staphylococcus aureus by employing DNA marker technology

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Naima Mahreen, Aasma Akhtar, Javaria Altaf, Muhammad Saqib, Mian Abdul Hafeez, Faisal Saeed Awan.
Molecular genotyping of methicillin-resistant Staphylococcus aureus by employing DNA marker technology.
J. Bio. Env. Sci. 10(6), 330-335, June 2017.
http://www.innspub.net/jbes/molecular-genotyping-methicillin-resistant-staphylococcus-aureus-employing-dna-marker-technology/
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