Isolation, identification, and charerization: Entomopathogenic fungi to Blattella germanica, Linnaeus (Orthoptera: Blattida)
By: Yayan Sanjaya, Wardono Nikoperbowo, Teguh Prasetyo
Key Words: Blattella germanica, Isolation, Identification, Pathogencity test, Entomopatogenic fungi.
J. Bio. Env. Sci. 8(6), 205-212, June 2016.
Isolation, identification and pathogenicity test entomopathogenic fungi against B. Germanica have been conducted. This study aims is to gain entomopathogenic fungal isolates derived from B. germanica, identify entomopathogenic fungal isolates obtained from B. germanica, and tested the pathogenicity of isolates of entomopathogenic fungi against B. germanica. The study was conducted by isolating the fungus derived from B. germanica were planted on the ground, then cultured and purified in an artificial medium Potato Dextrose Agar (PDA). Identification is done macroscopically by looking at pure culture of fungal isolates in Petri disc and microscopically by making culture slides were observed using a microscope. Pathogenicity test is done by applying a spore suspension on the entire body apart B. germanica and placed individually in sterile vial bottle. The results of isolation and identification of fungal isolates obtained five different types consisting of two genera, namely Mortierella and Aspergillus. Based on the results of pathogenicity test, all isolates of fungi were isolated have the ability to turn off B. germanica so that it can act as entomopathogenic fungus. The percentage value mortality fungal isolates 1, 2, and 3 is 40%. Value fungal isolates 4 percentage mortality is 60%. Isolates have value 5 percentage mortality by 20%.