Genotoxicity and oxidative stress analysis in the Catla catla treated with ZnO NPs
By: Muhammad Saleem Asghar, Naureen Aziz Quershi, Farhat Jabeen, Muhammad Shakeel, Muhammad Saleem Khan
Key Words: ZnO, Nanoparticles, genotoxicity, oxidative stress.
J. Bio. Env. Sci. 8(4), 91-101, April 2016.
In the history of human beings nanotechnology is one of the fastest growing industries and has been referred to as next industrial revolution. Zinc oxide nanoparticle (ZnO NPs) high concentration causes genotoxicity in the aquatic animals which is mainly due to the excess production of the oxidative stress. The treatment of ZnO NPs produces micronuclei comet and oxidative stress in the dose dependant manners. In the present study, the evaluation of genotoxicity was observed by micronuclei test and comet assay. The result showed that the increase concentration of ZnO NPs increase of the frequency of micronuclei and comets significantly. High frequency of comets (22.51±0.62) and micronuclei (0.557±0.081) was recorded at 80 mgL-1 after 28 days of treatment. This might be due to depletion of genetic repair mechanism. The oxidative stress was evaluated by observing the levels of SOD (Superoxide dismutase), CAT (Catalase), GST (Glutathione S-transferase), MDA (Malonetialdohyde content) and GSH (Reduce glutathione) after 7and 28 days of the ZnO NPs treatment. The gills and liver tissues exhibited a decrease in the activity of CAT and GST at every treatment in a dose dependent manner. However, the activity of SOD increased in response to ZnO NPs as MDA and GSH. This study concluded, ZnO NPs are genotoxic in the aquatic organisms and produce the oxidative stress at the elevated level.