International network for natural sciences – research journal
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Genetic diversity in some euphrates poplar (Populus euphratica O.) ecotypes in Iran using microsatellites (SSRs) markers

By: Hossein Tavakoli-Neko, Anoushirvan Shirvany, Mohammad Hassan Assareh, Mohammad Reza Naghavi,Mohammad Pessarakli

Key Words: Genetic variation, Populus euphratica, SSR marker.

J. Bio. Env. Sci. 9(1), 434-440, July 2016.

Abstract

Euphrates poplar (Populus euphratica Oliv.) is a unique woody species which is naturally distributed in desert areas of some parts of Asia and Africa. Because of its outstanding features, it is a model plant to study environment stress tolerance. This research was conducted to evaluate the genetic variation in 12 ecotypes of P. euphratica in Iran through 10 simple sequence repeats (SSRs) primers from 2015 to 2016. The average numbers of alleles observed in each ecotype was 6.43 and average numbers of effective alleles was 5.58. The average of observed heterozygosis was 0.65 and average of expected heterozygosis was 0.80. The ecotypes were complying with Hardy-Weinberg’s equilibrium in all loci, except Marand ecotype for two of the ten primers that showed deviation of the balance (p<0.05). The Shannon information index was 1.75. Analysis of molecular variance (AMOVA) showed that 3% of molecular variance belongs to intra-population and 97% belongs to inter-population. The PCA showed six principal components covered 22.86% of the total variance. Clustering analysis of ecotypes through genetic distance, the examined ecotypes were divided into six groups, while the geographic distance did not have any significant effect on genetic differences. Overall, the results indicated that P. euphratica stands covered a vast area of Iran in the past, and probably had not been fragmented; it seems vast areas of Iran are potentially ready for revival of P. euphratica forests.

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