International network for natural sciences – research journal
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Genetic diversity in Melissa officinalis accessions by leaf protein patterns

By: Zahra Danaeipour, Mohammad Hossein Fotokian, Daryush Talei

Key Words: Genetic diversity, Medicinal plants, Melissa officinalis, Protein pattern, SDS-PAGE.

J. Bio. Env. Sci. 8(5), 88-96, May 2016.

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Abstract

Lemon balm is a medicinal plant belongs to the family Lamiaceae. Proteins are important biochemical
parameters in genetic diversity in plants. The objective of the present study was to evaluate 20 accessions of M.
officinalis in terms of leaf protein banding patterns. For this reason, the leaf protein was extracted using HEPES/KOH buffer and separated by SDS-PAGE technique. The results indicated a total of 22 bands ranged from 10 to 150 kDa which 59.09 percentages of them were polymorphic. The maximum number of the bands (20) was observed in Germany and Malard accessions, while N. Khorasan accession had the minimum number of bands (13). The cluster analysis of 20 accessions based on the protein data produced three clusters. The first cluster comprised of 15 accessions, the second cluster consisted of four accessions and the third cluster contains only Italy accession. Overall, the outcomes of the present study were indicated the presence of high genetic diversity among the M. officinalis accessions. Our findings suggest that the plants belong to different clusters can be used for improvement of M. officinalis through hybridization to generate useful recombinants in the segregating generations.

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