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Evaluation of in-vitro antibacterial activity of leaf extracts of three species of family Oleaceae

By: Rizwana Rafique, Zaheer-Ud-Din Khan, Sundas Altaf, Anjum Parveen

Key Words: Invitro antibacterial activity, Jasminum sambac (L.) Aiton, Olea europaea L., Olea ferruginea Royle, agar well diffusion, MIC.

J. Bio. Env. Sci. 9(1), 150-158, July 2016.

Abstract

The present study was carried out to determine the antibacterial potential of the extracts of Jasminum sambac (L.) Aiton, Olea europaea L. and O. ferruginea Royle leaf against Staphylococus aureus and Bacillus subtilis (Gram positive bacteria) and. Escherichia coli and Pseudomonas aeruginosa (Gram negative bacteria) by agar well diffusion method. The extracts were obtained by maceration in solvents, such as n-hexane, chloroform, ethanol and distilled water. The zone of inhibition of the leaf extracts recorded as antibacterial potential was compared with that of the different standard antibiotics, like amikacin (30µg), erythromycin (10µg) etc. The n-hexane extracts of Jasminum sambac showed highest activity (26.37±2.54 mm) against E. coli. Aqueous and ethanol extracts of all the plants exhibited comparatively higher antibacterial potential against Gram negative bacteria than the Gram positive bacteria. The Minimum Inhibitory Concentration (MIC) was also determined for all the extracts. The results supported the ethnomedicinal use of Jasminum sambac (L.) Aiton, Olea europaea L. and O. ferruginea Royle to treat various bacterial infections in man.

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