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Effects of Moringa oleifera leaf meal (MOLM) based diets on carcass composition and hematology of Labeo rohita fingerlings

By: Muhammad Zubair-Ul-Hassan Arsalan, Syed Makhdoom Hussain, Muhammad Asrar, Haseeb Anwar, Muhammad Masoom-Ul-Hassan Rehan, Muhammad Mudassar Shahzad, Danish Riaz, Nisar Ahmad, Naveeda Wahab

Key Words: Labeo rohita, Proximate composition, Hematology, MOLM.

J. Bio. Env. Sci. 9(1), 214-223, July 2016.


A 70 – day feeding trial was carried out to study the effect of replacing the dietary fish meal with Moringa oleifera leaves meal (MOLM) on the carcass composition and hematological indices of Labeo rohita fingerlings. Five iso-nitrogenous diets were formulated with MOLM replacing 0%, 10%, 20%, 30% and 40% of fish meal in the diets. Fish were fed at 5% of their body weight two times daily. Fingerlings (avg. wt. 6.61±0.053g) were randomly distributed into tanks having 15 fish in each replicate. Results from proximate analysis showed that replacement of fish meal with MOLM upto 10% increased crude protein and crude fat in fish body as compared to fish fed on control (0%), 20%, 30% and 40% MOLM based diet, respectively. The hematological parameters of fingerlings fed 10% MOLM based diet were found to be significantly different (p<0.05) from the fish fed control diet. The red blood cells and hemoglobin of fish showed a significant (P<0.05) inverse correlation with increase in MOLM in diets. The present study showed that MOLM has good prospective for use as fish meal replacement in L. rohita diet up to 10% level without compromising fish performance whereas the hematological investigation indicated that inclusion of MOLM above 20% in the diets showed the hematological disturbance. On the base of this study it was concluded that fish meal can be replaced with MOLM up to 10% in the diets to increase the nutritive values of L. rohita fingerlings.


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