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Effect of salicylic acid on the expression of a gene involved in the biosynthesis of hyperforin in St. John’s wort (Hypericum perforatum)

By: Mina Shahim-Ghalecheh, Nader Farsad-Akhtar, Nasser Mahna, Jafar Razeghi

Key Words: Gene expression, HpPKS1, Salicylic acid, St. John’s wort

J. Bio. Env. Sci. 11(1), 63-68, July 2017.

Certification: jbes 2017 0009

Abstract

To address the role of the gene HpPKS1 in the biosynthesis of hyperforin in St. John’s wort, we analyzed the effect of different concentrations of salicylic acid on the expression of this gene via qRT-PCR method. To do this, the calli resulted from the leaf explants of in vitro grown plants were treated under three concentrations of salicylic acid (0, 100 and 250 µM) and the expression pattern of the gene HpPKS1 was evaluated after 24, 48 and 72 hours. The results demonstrated that with increasing the concentration of salicylic acid, the expression of the gene showed a significant increment 24 and 48 hours after treatment; while after 72 hours a decrease was observed in all concentrations. Furthermore, in all time durations, the expression of HpPKS1 was increased with raising the concentration of salicylic acid, so that the maximum activity of the gene was witnessed at 250 µM salicylic acid.

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Effect of salicylic acid on the expression of a gene involved in the biosynthesis of hyperforin in St. John’s wort (Hypericum perforatum)

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Mina Shahim-Ghalecheh, Nader Farsad-Akhtar, Nasser Mahna, Jafar Razeghi.
Effect of salicylic acid on the expression of a gene involved in the biosynthesis of hyperforin in St. John’s wort (Hypericum perforatum).
J. Bio. Env. Sci. 11(1), 63-68, July 2017.
http://www.innspub.net/jbes/effect-salicylic-acid-expression-gene-involved-biosynthesis-hyperforin-st-johns-wort-hypericum-perforatum/
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