International network for natural sciences – research journal
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Direct embryogenesis of Valerian (Valeriana officinalis L.) using leaf segments

By: Arsalan Shekarchian, Ghollam Hossein Rahmani, Mohammad Reza Kudori, Davod Darvishi Zeidabadi

Key Words: Direct embryogenesis, In vitro, Tissue culture, Valeriana officinalis.

J. Bio. Env. Sci. 9(6), 113-120, December 2016.

Abstract

A simple procedure for direct embryogenesis without an intervening callus production was developed in valerian (Valeriana officinalis L.), using the leaf segments. Direct somatic embryogenesis was induced using half-strength Murashige and Skoog (MS) medium supplemented with 2, 4 dichlorophenoxyacetic acid (0.5mg L-1), glutamic acid (100mg L-1), 4% sucrose and 8g L-1 agar. Embryo germination to form plantlets enhanced on MS medium supplemented with naphthalene acetic acid (0.1mg L-1) and kinetin (2mg L-1). Regenerated plants with well-developed root and shoot systems were successfully transferred to greenhouse.

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