International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Comparative study of biochemical characteristics of halophytes from two different habitats

By: Badridze Gulnara, Kacharava Nani, Chkhubianishvili Eva, Rapava Luara, Kikvidze Medea, Chigladze Lali, Chanishvili Shota

Key Words: Salt stress, Antioxidants, Halophytes

J. Bio. Env. Sci. 11(5), 98-116, November 2017.

Certification: jbes 2017 0104 [Generate Certificate]

Abstract

The worldwide problem of soil salinization may be solved by amelioration of saline soils, or by cultivation and selection of salt resistant plants. That’s why investigation of wild halophytes gains popularity. One of the approaches in salt-resistance studies is investigation of morphological, physiological and biochemical mechanisms of adaptation to salt stress. From this point of view, halophytes growing in Georgia are practically unexplored. The purpose of the study was to investigate the activity of enzymes (peroxidase and nitrate reductase), and content of low molecular antioxidants (proline, soluble phenols, anthocyanins, carotinoides and ascorbic acid), as well as nitrates, soluble carbohydrates and total proteins in leaves of salt resistant species growing in different salty habitats of Georgia – the costal zone and near-by territory of Kumisi (east Georgia, kvemo Kartli) and Sakhare-Kapanadze lakes (east Georgia, gare Kakheti). Titration (ascorbic acid) and spectrophotometric methods were applied for investigations. There may be distinguished some biochemical indices, in spite of the salt exchange mechanism type of tested species, which changed by the same regularity following habitats: content of ascorbic acid (1.3-2.5 times, p<0.05), total phenols (1.2-3.6 times, p<0.05) and soluble carbohydrates (2-times and more, p<0.05) was higher in species of Sakhare-Kapsanadze lakes, compared to Kumisi same species; while in all species of Kumisi habitat content of total proteins was higher (2.7-5 times, p<0.05). Generally, adaptation of the tested species to studied habitats was much or less of individual character.

| Views 64 |

Comparative study of biochemical characteristics of halophytes from two different habitats

Andreeva VA. 1988. Peroxidaze – participation in plant’s defence mechanisms. Moskow. Nauka, 7-24.

Apkhazava IS. 1975. Lakes of Georgia. Tbilisi. Metsniereba (in Russian).

Bagheri M. 2014. The effect of maize priming on germination characteristics, catalase and peroxidase enzymc activity, and total protein content under salt stress. International Journal of biosciences (IJB) 4, 104-112.

Bates LS, Waldren RP, Treare ID. 1973. Rapid determination of free proline for water-stress studies. Plant and Soil 39, 205-207.

Bhutta WM. 2011. Antioxidant activity of enzymatic system of two different wheat (Triticum aestivum L.) cultivars growing under salt stress. Plant Soil and Environment 57, 101-107.

Boestfleisch C, Wagenseil NB, Buhmann AK, Seal CE, Wade EM, Muscolo A. 2014. Manipulating the antioxidant capacity of halophytes to increase their cultural and economic value through saline cultivation. AoB Plants 6, plu0 46.

http://dx.doi.org/10.1093/aobpla/plu046.

Caverzan A, Passaia G, Barcellos Rosa S, Werner Ribeiro C, Lazzarotto F, Margis-Pinheiro M. 2012. Plant responses to stresses: Role of ascorbate peroxidase in the antioxidant protection. Genetics and Molecular Biology 35, 1011–1019.

Cesar G, Fraga CG. 2010. Plant Phenolics and Human Health: Biochemistry, Nutrition, and Pharmacology.  John Wiley & Sons, Inc.

Cevahir G, Yentur S, Yazgan M, Unal M, Yilmazer N. 2004. Peroxidase activity in relation to anthocyanin and chlorophyll content in juvenile and adult leaves of “mini-star” Gazanla splendens. Pakistan Journal of Botany 36(3), 603-609.

Chalker-Scott L. 2002. Do anthocyanins function as osmoregulators in leaf tissues? In: Gould KS, Lee DW eds. Anthocyanins in Leaves. Amsterdam, Academic Press.

Chkhubianishvili E, Badridze G, Kacharava N, Rapava L, Kikvidze M, Chanishvili Sh, Chigladze L. 2016. Enzymes activity and content of antioxidants in leaves of halophytes from saline soils of Kumisi lake.  International Journal of Agronomy and Agricultural Research (IJAAR) 9(1), 32-46.

Couee I, Sulmon C, Gouesbet G, El Amrani A. 2006. Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants. Journal of Experimental Botany 57(3), 449-459.

Dajic Z. 2006. Salt stress. In: Madhava Rao KV, Raghavendra AS. Janardhan Reddy, K. (eds.) Physiology and molecular biology of stress tolerance in plants. Netherlands, Springer.

Danilova NS. 1963. Determination of nitrates in plant material. Fiziologia rasteniy, 10(4), 497-498.

Datta KS, Kumar A, Varma SK, Angrish R. 1995. Differentiation of chloride and sulphate salinity on the basis of ionic distribution in genetically diverse cultivars of wheat. Journal of Plant Nutrition 18, 2199–2212.

Eriomchenko OZ, Chudinova LA, Kusakina MG, Shestakov IE. 2013. Accumulation of osmolites in plants with different mechanisms of adaptation to salinization. Recent problems in science and education 2, 424.

Ermakov AI. ed. 1987. Methods of biochemical analysis of plants. Leningrad, .Agropromizdat.

Ferraris L, Abbatista-Gentile I, Matta A. 1987. Variations of phenolics concentrations as a consequence of stress that induce resistance to Fusarium wilt of tomato. Journal of Plant Diseases and Protection 94, 624-629.

Fiedor J, Burda K. 2014. Potential Role of Carotenoids as Antioxidants in Human Health and Disease.  Nutrients 6, 466–488.

Franko OL, Melo FR. 2000. Osmoprotectors: plant response on osmotic stress. Russian Journal of Plant Physiology 47(1), 152-159.

Gallardo K, Courty P-E, Le Signor C, Wipf D, Vernoud V. 2014. Sulfate transporters in the plant’s response to drought and salinity: regulation and possible functions.  Frontiers in Plant Science 5, 580. http://dx.doi.org/10.3389/fpls.2014.00580.

Garg BK, Kathju S, Vyas SP, Lahiri AN. 1997. Sensitivity of clusterbean to salt stress at various growth stages. – Indian Journal of Plant Physiology 2, 49-53.

Garg N, Singla R. 2005. Nitrate reductase activity in roots  and leaves of chickpea cultivars under salt stress. Spanish Journal of Agricultural Research 3(2), 248-252.

Gavrilenko VF, Ladigina ME, Khandobina LM. 1975. Big practical handbook in plant physiology.  Moscow, Bisshaia shkola. 127-134.

Gill SS, Tuteja N. 2010. Reactive oxygen species and antioxidant   machinery in abiotic stress toletance in crop plants.  Plant Physiology and Biochemistry 48(12), 909-30.

Gogue DO. 2014. The physiological mechanisms of salt resistance of two species of the genus Nigella and some characteristics of the quality of their raw material. Thesis of the candidate dissertarion. Moscow, 115p (In Russian).

Gould KS. 2004. Nature’s Swiss Army Knife: The Diverse Protective Roles of Anthocyanins in Leaves. Journal of Biomedicine and Biotechnology 5, 314– 320.

Graskova IA, Zhivetyev MA, Putalina TE, Krasnobaev VA, Voinikov VN. 2010. Activity and izoenzyme spectrum of peroxidase of some herbaceous plants from the bank of lake Baikal, growing under abiotic stress. The electronic scientific journal “Issledovano v Rosii” (Investigated in Russia) 293-303.

Guliev AG, Samofalova IA, Mudrikh NM. 2014. Salinization – the global ecological problem in irrigating agriculture.  Permskiy Agrarniy Vestnik 4 (8), 32-43 (in Russian).

Hemavathi CP, Upadhyaya KE, Young N, Akula KE, Young SC, Chun DHK, Park SW. 2010. Enhanced ascorbic acid accumulation in transgenic potato confers tolerance to various abiotic stresses.  Biotechnology Letters 32, 321-330.

Kafi M, Stiuart BS, Borland AM. 2003. Content of carbohydrates and proline in leaves, roots and apices of resistant and sensitive to salinity varieties of wheat.  Russian Journal of Plant Physiology 50(2), 174-182.

Kahkonen MP, Heinonen M. 2003. Antioxidant activity of anthocyanins and their aglycons.  Journal of Agricultural and Food Chemistry 51(3), 628-633.

Kartashov AV. 2013. Significance of morphophysiological peculiarities of Plantago major and P. maritima for supporting the salt and water balance in case of salinization. Thesis of candidate dissertation. Moscow.

Katyar S, Dubey RS. 1992. Influence of NaCl salinity on behaviours of nitrate reductase and nitrite reductase in rice seedlings differing in salt tolerance.  Journal of Agronomy and Crop Science 169, 289-297.

Khan MG. 1996. Nitrate and nitrite reductase activities in soybean plants raised with saline water.  Indian Journal of Plant Physiology 1(2), 128-129.

Koleva II, van Beek TA, Linssen JP, de Groot A, Evstatieva LN. 2002. Screening of plant extracts for antioxidant activity: A comparative study on three testing methods. Phytochemical Analysis 13, 8-17.

Kosakivska I, Klymchuk D, Negretzky V, Bluma D, Ustinova A. 2008. Stress proteins and ultrastructural characteristics of leaf cells of plant with different types of ecological strategies.  General and Applied Plant Physiology 34(3-4), 405-418.

Ksouri R, Megdiche W, Debez A, Falleh H, Grignon C, Abdelly C. 2007. Salinity effects on polyphenol content and antioxidant activities in leaves of the halophyte Cakile maritime.  Plant Physiology and Biochemistry 45, 244-249.

Kusakina MG, Gotiachevskaya TN, Chetina OA. 2011. Influence of different level technogenic salinization on some metabolic indices of plant. Proceedings of Permi University, biological series 1, 73-77.

Kuznetsov VlV, Sheviakova NI. 1999. Proline under stress: biological role, metabolism, regulation. Russian Journal of Plant Physiology 46(2), 321-336.

Kuznetsova SA, Klimachev DA, Kartashov SN, Starikova VT. 2014. Influence of salinization on the indices of photosynthetic activity of plants.   Proceedings of Moscow State University, Series “Natural sciences” 1, 63-68.

Lichtenthaller HK, Buschmann C. 2001. Chlorophylls and Carotenoides: Measurement and Characterization by UV-VIS Spectroscopy. Current protocols in food analytical Chemistry. F4.3.1-F4.3.8.

Lowry OH, Rosebrough NT, Farr AL, Randall RJ. 1951. Protein measurement with the folin phenol reagent.  Journal of Biological Chemistry 139, 256-275.

Magistad OC,  Ayers AD,  Wadleigh CH, Gauch

  1. 1943. Effect of salt concentration, kind of salt and climate on plant growth in sand cultures. Plant Physiology 18(2), 151–166.

Manousaki E, Kalogerakis N. 2011. Halophytes Present New Opportunities in Phytoremediation of Heavy Metals and Saline Soils. Industrial and Engineering Chemistry Research 50, 656–660.

Marschner H. 1995. Mineral Nutrition of Higher Plants. San Diego, Academic Press.

Martin R, Aghajanzadeh T, Elisabeth C, Stuive E, Koralewska A, De Kok LJ. 2015. Impact of Sulfate Salinity on the Uptakeand Metabolism of Sulfur in Chinese Cabbage.  In: Luit J.   De Kok Malcolm  J.   Hawkesford H.  Rennenberg  K. Saito E. Schnug eds. Molecular Physiology and Ecophysiology of Sulfur.  Proceedings of the international sulfur workshop. Switzerland, Springer International Publishing.

McNeil SD, Nuccio ML, Hanson AD. 1999. Betaines and Related Osmoprotectants. Targets for Metabolic Engineering of Stress Resistance. Plant Physiology 120(4), 945-949.

http://dx.doi.org/10.1104/pp.120.4.945

Mobin M, Khan NA. 2007. Photosynthetic activity, pigment composition and antioxidative response of two mustard (Brassica juncea) cultivars differing in photosynthetic capacity subjected to cadmium stress. Journal of Plant Physiology 164(5), 601-610.

Mohammadkhani N, Heidari R. 2008. Drought-induced Accumulation of Soluble Sugars and Proline in Two Maize Varieties. – World Applied Sciences Journal 3(3), 448-453.

Munns R, Tester M. 2008. Mechanisms of Salinity

Tolerance. – Annual Review of plant Biology 59(1), 651-681. http://dx.doi.org/10.1146/annurev.arplant.59.032607.092911

Munns R, Husain S, Rivelli AR, James RA, Condon AG, Lindsay MP, Lagudah ES, Schachtman DP, Hare RA. 2002. Avenues for increasing salt tolerance of crops, and the role of physiologically based selection traits. Plant and Soil 247(1), 93–105.

Orlova NV, Kusakina MG, Suchkova NV. 2007. Relation between content of water-soluble proteins in halophytes and soil salinization level. Proceedings of Permi University 5(10), 31-34.

Passardi F, Cosio C, Penel C, Dunand C. 2005. Peroxidases have more functions than a Swiss army knife.  Plant Cell Reports 24(5), 255-265.

Pleshkov BP. 1985. Practical handbook of plant biochemistry., Moscow, Kolos.

Prado FE, Boero C, Gallarodo M, Gonzalez JA. 2000. Effect of NaCl on germination, growth and   soluble sugar content in Chenopodium  quinoa Willd. seeds.  Botanical Bulletin of Academy Sinica 41, 27-34.

Renault S, Croser C, Franklin JA, Zwiazek JJ. 2001. Effects of NaCl and Na2SO4 on red-osier dogwood (Cornus stolonifera  Michx) seedlings. Plant and Soil 233, 261–268.

Rezazadeh A, Ghasemnezhad A, Barani M, Telmadarrehei T. 2012. Effect of Salinity on Phenolic Composition and Antioxidant Activity of Artichoke (Cynara scolymus L.) Leaves.  Research Journal of Medicinal Plant 6, 245-252.

Rozentsvet OA, Nestorov VN, Bogdanova ES. 2013. The structural and functional characteristic of halophytes photosynthetic apparatus, with different salt-accumulating mechanism. Proceedings of Samara Scientific Center, Russian Academy of Sciences 15, 3(7), 2189-2195.

Saakashvili N, Tarkhan-Mouravi I, Tabidze M,

Kutateladze N. 2011. Georgian curortographgy and curort theraphy. Tbilisi, Sakartvelos Matsne.

Sergeichik SA, Sergeichik AA. 1988. Influens of gaseous industrial toxins on the activity of peroxidase and nitrate reductase in leaves of woody plants.  Thesis of the reports of republic workshop (16-18 February, Jurmala) Riga, 124.

Shao HB, Chu IY, Lu ZHH, Kang CM. 2008. Primary Antioxidant Free Radical Seavenging and Redox Signating Pathways in Higher Plant. Cell. International Journal of Biological Science 4, 8-14.

Szabados L, Savouré A. 2010. Proline: a multifunctional amino acid.  Trends in Plant Sciences 15, (89-97).

Turkina MV, Sokolova SV. 1971. Methods of determination of mono- and oligosaccharides. – In: Pavlinova OA. ed. Biological methods in plant physiology. Moscow, Nauka, 226 p.

Urushadze TF, Blum W. 2014. Soils of Georgia. Tbilisi, Mtsignobari, 269-278.

Ventura Y, Sagi M. 2013. Halophyte crop cultivation: The case for Salicornia and Sarcocornia. – Environmental and Experimental Botany 92, 144-153.

Vwioko ED, Osawaru ME, Eruogun OL. 2008. Evaluation of okro (Abelmoschus esculentus L. Moench.) exposed to paint waste contaminated soil for growth, ascorbic acid and metal concentration. – African Journal of General Agriculture 4, 39–48.

Woo SH. 2010. Abiotic stress responsive proteins of  wheat grain determined using proteomics technique.

Australian Journal of Crop Sciences 4(3), 196-208.

Wu X, Beecher GR, Holden JM, Haytowitz DB, Gebhardt SE, Prior RL. 2004. Lipophilic and hydrophilic antioxidant capacities of common foods in the United States. Journal of the Agricultural and Food Chemistry 52, 026–4037.

Zhang L, Zang G, Wang I, Zhou Z, Meng I, Chen B. 2013. Effect of soil salinity on physiological characteristics of functional leaves of cotton plants. Journal of Plant Research 126(2), 293-304.

Zhang Z, Wang J, Zhang R, Huang R. 2012. The ethylene response factor AtERF98 enhances tolerance to salt through the transcriptional activation of ascorbic acid synthesis in Arabidopsis. Plant Journal 71(2), 273-287.

Badridze Gulnara, Kacharava Nani, Chkhubianishvili Eva, Rapava Luara, Kikvidze Medea, Chigladze Lali, Chanishvili Shota.
Comparative study of biochemical characteristics of halophytes from two different habitats.
J. Bio. Env. Sci. 11(5), 98-116, November 2017.
http://www.innspub.net/jbes/comparative-study-biochemical-characteristics-halophytes-two-different-habitats/
Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
http://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update