International network for natural sciences – research journal
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Carcass composition and hematological study of Catla catla fingerlings fed on phytase supplemented Moringa oleifera leaf meal (MOLM) based diet

By: Muhammad Mudassar Shahzad, Syed Makhdoom Hussain, Farhat Jabeen, Abdullah Ijaz Hussain, Muhammad Zubair-Ul-Hassan Arsalan, Nisar Ahmad, Muhammad Masoom-Ul-Hassan Rehan, Danish Riaz

Key Words: Carcass, Catla catla, MOLM, Phytase, Hematology.

J. Bio. Env. Sci. 9(1), 57-68, July 2016.

Abstract

Present study was conducted to investigate carcass composition and hematological parameters of Catla catla fingerlings fed on phytase supplemented Moringa oleifera leaf meal (MOLM) based diet. Graded levels (0, 300, 600,900, 1200 and 1500 FTU kg-1) of phytase enzyme were used to prepare one control and five test diets. Catla catla fingerlings were fed twice a day at the rate of 4% of wet weight. After 90 days trial, four fish from each tank were sacrificed after taking blood from caudal vein to analyze hematological parameters. Fish fed MOLM based test diet supplemented with phytase at 900 FTU kg-1 level showed maximum retention of crude protein and crude fat in fish body as compared to fish fed on control diet (without phytase supplementation). Carbohydrate, crude fiber, moisture and ash values were highest at control diet whereas lowest contents were recorded at 900 FTU kg-1 level. Results of hematological study also showed that the maximum values of RBCs (2.66±0.08 106mm-3), WBCs (7.68±0.09 103mm-3) and Hb (8.74±0.17 g/100ml) were found at 900 FTU kg-1 level of phytase supplementation. Whereas highest PCV and PLT count was observed at 600 FTU kg-1. It was concluded that use of phytase at 900 FTU kg-1 level improved carcass composition and hematological parameters of C. catla fingerlings without any side effect on fish performance.

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