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Assessment of clove oil and Benzocaine Anaesthesia on Haematological and Histopathological profile of Haludaria fasciata

By: Baiju A. Padiyoor, F. G. Benno Pereira, V. Jayaprakas

Key Words: Blood profile, Histology, Melon barb, Stress response, Optimal dose

J. Bio. Env. Sci. 10(4), 27-37, April 2017.

Abstract

Transportation of live fish is a stressful procedure. Present study assesses the optimal concentration and the degree of effectiveness of clove oil and benzocaine as anaesthetics separately for stress reduced transportation of Haludaria fasciata. Study contributes the knowledge on some aspects of stress research include the detection of histological and haematological alteration as a biomarkers for evaluating degree of pollution or toxic effect with the environment of an organism. The effects anaesthetics revealed at some stages (10 min and 24 hr) of experiment. Histology of gills and liver; and haematological parameters included the erythrocyte count (RBC), leukocyte count (Leuko,TC), haemoglobin concentration (Hb), haematocrit (PCV – Packed Cell Volume) as well as mean erythrocyte volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were assessed for determine the effect of clove oil and banzocaine on H. fasciatus and compared with nonanaesthetised control group. Both the anaesthetics do not cause to irreversible damage in the test fishes. The effects of benzocaine on fish were comparatively higher than clove oil treatments. The degree of alteration may due to the defensive response with additives in external environment. The obtained result shows that the optimal dose of clove oil (50 µl) and benzocaine (30 µl) anaesthesia are effective, safe and recommended as suitable anaesthetics for the transportation of H. fasciatus.

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Assessment of clove oil and Benzocaine Anaesthesia on Haematological and Histopathological profile of Haludaria fasciata

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Baiju A. Padiyoor, F. G. Benno Pereira, V. Jayaprakas. 2017. Assessment of clove oil and Benzocaine Anaesthesia on Haematological and Histopathological profile of Haludaria fasciata. J. Bio. Env. Sci. 10(4), 27-37.
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