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Lignocellulolytic activities of culturable marine woodborers’ gut microbiota

By: Bosire Carren M, Abubakar Laila U

Key Words: Lignocellulolytic enzyme, Woodborer, Gut microbiota

Int. J. Micro. Myco. 5(5), 1-18, May 2017.


In this study, pure bacterial and fungal isolates obtained by culturing inoculum from woodborers’ gut were induced to produce lignocellulolytic enzymes in a variety of substrates and their lignocellulolytic activities investigated. The inocula were obtained from woodborers Dicyathifer mannii (Wright, 1866), Sphaeroma terebrans (Bate, 1866) and Cirolana sp. The cultures used enrichment media containing ground Rhizophora mucronata wood, filter paper, carboxyl methylcellulose, avicel cellulose, beechwood xylan and cellobiose as sole carbon sources. The microorganisms showed generally low ligninolytic activities but commendable cellulolytic and hemicellulolytic activities. β-glucosidase and xylanase activities were the highest activities exhibited by both bacterial and fungal isolates. The highest was bacterial β-glucosidase activity (94.55 U/ml) shown by Lysinibacillus boronitolerans from S. terebrans gut cultured in a medium containing avicel cellulose. Xylanase activity was also relatively high (up to 91.7U/ml) cultured in media containing cellobiose and xylan. The fungal isolate with the highest cellulolytic activity was β-glucosidase activity of 38.34 U/ml shown by Aspergillus niger obtained from the gut of S. terebrans and cultured in a medium containing avicel cellulose as a sole carbon source. Avicel cellulose, cellobiose and xylan beech wood were found to be best inducers of cellulase and hemicellulase production. Lysinibacilli and Aspergilli in this study present interesting advantages that make them good models for studying physiological approaches to enzyme production and lignocellulose degradation.

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Lignocellulolytic activities of culturable marine woodborers’ gut microbiota

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Bosire Carren M, Abubakar Laila U.
Lignocellulolytic activities of culturable marine woodborers’ gut microbiota.
Int. J. Micro. Myco. 5(5), 1-18, May 2017.
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