International network for natural sciences – research journal
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Bacteriological profile and antibiogram of blood culture isolates from patients of rural tertiary care hospital

By: S. Oza Sweta, J. Mehta Sanjay, M. Kunjan Kikani, G. Oza Sunil

Key Words: Antibiotics, Bacteremia, Blood culture

Int. J. Micro. Myco. 4(3), 1-7, October 2016.


Microbial invasion of blood stream is associated with significant mortality and morbidity. Identification of bacterial isolates and antibiotic susceptibility of bacteria isolated from blood culture would guide the antibiotics treatment for patients with bacteremia. 1) To determine age – wise blood culture positivity rate in bacteremia 2) To identify age – wise common bacterial species isolates in bacteremia 3) To determine Antibiotic sensitivity pattern of the bacterial isolates. Atotal of 247 blood culture samples received from various clinical departments of rural teaching hospital from August 2013 to September 2015 were included in the study. Samples were collected in brain heart infusion broth. Identification of isolates and antimicrobial susceptibility was done as per standard microbiological methods. Out of 247 specimens bacteria sp. was isolated from 46 (18.62%) samples. Blood culture positivity was noted highest among neonates age group (38.71%). Lowest rate was observed among elders (4.55%). Klebsiella pneumoniae, Coagulase negative staphylococcus (CONs), and S. aureus were common blood culture isolates. In neonates Klebsiella pneumoniae was the most common isolate. Out of 27 gram negative bacilli, 14 (51.85%) were extended spectrum betalactamases (ESBL) positive. High resistance was noted against amoxycillin and amoxicillin/clavulanic acid and third generation cephalosporins in all gram negative organisms except, S. typhi. Out of 12 Staphylococcus sp., none of these were methicillin resistant. Routine antibiotic susceptibility surveillance helps in choice of antibiotics for treatment, identification of resistance and control of its spread.


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