In vitro organogenesis of Ageratum houstonianum (Asteraceae)
By: Mastaneh Mohammadi
Key Words: Ageratum houstonianum, Kin and IAA, Regeneration, Tissue culture
Int. J. Biosci. 10(5), 85-96, May 2017.
Regeneration of whole plants from tissue explants is a key technology for crop improvement and plant biotechnology and has critically contributed to the green revolution. In this study, the effects of MS medium supplemented with various concentrations of Kin and IAA combination on induction of callus, shoot and root induction and multiplication, and plantlet formation in various explants of Ageratum houstonianum were investigated. Maximum number of callus formation was observed in leaf at 1-1 and 4-4 (mg L−1), and in shoot apical meristem at 2-2 (mg L−1) of Kin and IAA combination. 0.4-1 (mg L−1) was the best combination for root and shoot regeneration on root and leaf explants, respectively. The maximum number of both root and shoot formation in epicotyl explant was observed at 2-4 (mg L−1). Among the explants, shoot apical meristems were responding for regeneration of plantlets, and the optimum concentration of Kin-IAA to produce plantlet was 4-2 (mg L−1) combination. This study would be an important step towards the development of good quality of organogenesis to provide successful plantlet production from other Ageratum species.
In vitro organogenesis of Ageratum houstonianum (Asteraceae)
Abou-Mandour AA. 1999. Effect of exogenous growth regulators on organogenesis of tissue cultures of Ageratina riparia regel (Asteraceae). Angewandte Botanik 73(5), 162.
Amin S, Kaloo ZA, Singh S, Altaf T. 2013. Micropropagation of medicinally important plant species of family Asteraceae- A review. International Journal of Recent Scientific Research 4(8), 1296.
Anbazhagan M, Kalpana M, Rajendran R, Natarajan V, Dhanavel D. 2010. In vitro production of Stevia rebaudiana Bertoni. Emirates Journal of Food and Agriculture 22, 216.
Atta R, Laurens L, Boucheron-Dubuisson E, Guivarc’h A, Carnero E, Giraudat-Pautot V. 2009. Pluripotency of Arabidopsis xylem pericycle underlies shoot regeneration from root and hypocotyl explants grown in vitro. Plant Journal 57, 626.
Das A, Gantait S, Mandal N. 2011. Micropropagation of an elite medicinal plant: Stevia rebaudiana Bert. International Journal of Agricultural Research 6, 40.
Del Pozo JC, Lopez-Matas MA, Ramirez-Parra E, Gutierrez C. 2005. Hormonal control of the plant cell cycle. Physiologia Plantarum 123, 173.
Flores HE, Pickard JJ, Hoy MW. 1988. Production of polyacetylenes and thiophenes in heterotrophic and photosynthetic root cultures of Asteraceae. In: Lam J, Breteler H, Arnason T, Eds. Chemistry and Biology of Naturally Occurring Acetylenes and Related Compounds. Amsterdam: Elsevier, 233.
Flores HE, Dai Y, Cuello JL, Maldonado-Mendoza lE, Loyola-Vargas VM. 1993. Green Roots: Photosynthesis and Photoautotrophy in an Underground Plant Organ. Physiologia Plantarum 10, 1363.
Gordon SP, Heisler MG, Reddy GV, Ohno C, Das P, Meyerowitz EM. 2007. Pattern formation during de novo assembly of the Arabidopsis shoot meristem. Development 134, 3539.
Himanen K, Boucheron E, Vanneste S, Almeida Engler J, Inze D, Beeckman T. 2002.Auxin-mediated cell cycle activation during early lateral root initiation. Plant Cell 14, 2339.
Hwang SJ. 2006. Rapid in vitro propagation and
enhanced stevioside accumulation in Stevia rebaudiana Ber. Journal of Plant Biology 49(4), 267.
Jain P, Kachhwaha S, Kothari SL. 2009. Improved micropropagation protocol and enhancement in biomass and chlorophyll content in Stevia rebaudiana (Bert.) Bertoni by using high copper levels in the culture medium. Scientia Horticulturae 119, 315.
Juan C, Kaiwen P, Bin G, Jinchuang W, Yuehua W, Tao W. 2006. Atractylodes macrocephala – Rapid propagation by direct shoot and plant regeneration by leaf. Chinese Agricultural Science Bulletin 22, 65.
Khan M, Ahmad S. 2008. Pharmacognostical, phytochemical, biological and tissue culture studies on Parthenium hysterophorus Linn. A Review. The Internet Journal of Alternative Medicine 6(2).
Kumar N. 2014. Biological potential of a weed Ageratum houstonianum MILL: A review. Indo American Journal of Pharmaceutical Research (IAJPR) 4(6), 2683.
Kumar SP, Kumari BDR. 2010.Effect of Amino Acids and Growth Regulators on Indirect Organogenesis in Artemisia vulgaris L. Asian Journal of Biotechnology 1.
Kurade NP, Vikas J, Vijay KK, Sharma OP.2010.Chemical composition and antibacterial activity of essential oils of Lantana camara, Ageratum houstonianum and Eupatorium adenophorum. Pharmaceutical Biology 48(5), 539.
Laskowski M, Grieneisen VA, Hofhuis H, Hove CA, Hogeweg P, Maree AF. 2008. Root system architecture from coupling cell shape to auxin transport. PLoS Biology 6, 307.
Menut C, Lamaty G, Zollo PH, Kuiate JR,
Bessière JM. 1993.Aromatic plants of tropical central Africa. Part X chemical composition of the essential oils of Ageratum houstonianum Mill and Ageratum conyzoides L. from Cameroon. Flavour and Fragrance Journal 8, 1.
Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 473.
Nabi SA, Rashid MM, Al-Amin M, Rasul MG. 2002. Organogenesis in Teasle Gourd (Momordica dioica Roxb.). Plant Tissue Culture 12(2), 173.
Njateng GSS, Gatsing JRD, Tamokou JD, Mouokeu RS, Kuete V. 2010. Antidermatophytic Activity and Dermal Toxicity of Essential Oil from the leaves of Ageratum houstonianum (Asteraceae). Journal of Biological Sciences 10, 448.
Nokhasi J, Khosravi A, FayazMoghadam A. 2014.Effect of genotype and various hormonal combinations on regeneration of sunflower (Helianthus annuus L.). International Journal of Biosciences 5(10), 119.
Pandey DK, Chandra H, Tripathi NN, Dixit SN. 1984.Mycotoxicity in leaves of some higher plants with special reference to that of Ageratum houstonianum Mill. Mykosen 26, 565.
Panigrahi J, Mishra RR, Behera M. 2006. In vitro multiplication of Asteracantha longifolia (L.) Nees- A medicinal herb. Indian Journal of Biotechnology 5, 562.
Paulsamy S, Ganesan CM. 2011. Mass Propagation of a Threatened Medicinal Plant, Artemisia nilagirica (C.B. Clarke) Pampan Inhabiting high Hills of Nilgiris, the Western Ghats. Indian Journal of Fundamental and Applied Life Sciences 1(3), 14.
Pinto José EBP, De Souza Ana V, Bertolucci
Suzan KV, Corrêa Ricardo M. 2007. In vitro Propagation of Lychnophora pinaster (Asteraceae): A Threatened Endemic Medicinal Plant. HORTSCIENCE 42(7), 1665.
Ravindran J, Samuel T, Alex E, William J.2012. Adulticidal activity of Ageratum houstonianum Mill. (Asteraceae) leaf extracts against three vector mosquito species (Diptera: Culicidae). Asian Pacific journal of tropical disease 2(3), 177.
Rizvi SMD, Shakil S, Zeeshan M, Khan MS, Shaikh S, Biswas D, Ahmad A, Amjad M. 2014. An enzoinformatics study targeting polo-like kinases-1 enzyme: Comparative assessment of anticancer potential of compounds isolated from leaves of Ageratum houstonianum. Pharmacognosy Magazine 10(37), 14.
Sarin R, Bansal N. 2011. Impact of growth regulators on Callus production of two medicinal plants viz. Adhatoda vasica and Ageratum conyzoides. International Journal of Research in Plant Science 1(1), 1.
Senthilkumar P, Paulsamy S. 2010. Standardization of protocol for the propagation of the exotic medicinal herb, Ageratum conyzoides by employing tissue culture technology. Journal of Exotoxicology and Environment Monitoring 20(4), 393.
Sharma PD, Sharma OP. 1995.Natural products chemistry and biological properties of the Ageratum plant. Toxicological and Environmental Chemistry 50, 1.
Shoji K, Addicott FT, Swets WA. 1951.Auxin in relation to leaf blade abscission. Plant Physiology26, 189.
Siebertz R, Proksch R, Witte L. 1990. Accumulation and biosynthesis of the chromenes precocene 1 and 11 in Ageratum. Phytochemistry 29(7), 2135.
Srinivas RK, Sanjeeva KA, Ganapaty S. 2012.Evaluation of Ageratum houstonianum whole plant for its anti-diabetic activity. Journal of Advanced Scientific Research 3, 67.
Sujatha G, Kumari BDR.2007. Effect of phytohormones on Micropropagation of Artemisia vulgaris L. Acta Physiologiae Plantarum 29, 189.
Tamura Y, Nakamura S, Fukui H, Tabata M. 1984. Clonal propagation of Stevia rebaudiana Bertoni by stem-tip culture. Plant Cell Reports 3, 183.
Tedonkeng Pamo E, Tendonkeng F, Kana JR, Khan Payne V, Boukila B, Lemoufouet J, Miegoue E, Nanda AS. 2005.A study of the acaricidal properties of an essential oil extracted from the leaves of Ageratum houstonianum. Veterinary Parasitology 128(3-4), 319.
Tennyson S, Balaraju K, Kyungseok P, Raja AK, Ravindran KJ, Alex E, William SJ. 2011. In vitro antimicrobial activity of Ageratum houstonianum Mill. (Asteraceae). Food Science 35, 2897.
Tennyson S, Balaraju K, Park K, Ravindran J, Alex E, William J. 2012.In vitro antioxidant activity of Ageratum houstonianum Mill. (Asteraceae). Asian Pacific Journal of Tropical Disease 2(2), 712.
Tomar R, Tiwari S. 2006. In vitro morphogenesis of Rauwolfia serpentina L. through cotyledons. Plant Cell Biotechnology and Molecular Biology 7, 53.
Wiedenfeld H, Andrade-Cetto A. 2001. Pyrrolizidine alkaloids from Ageratum houstonianum Mill. Phytochemistry 71(1), 1269.
Xu L, Chen X, Qu Y, Sheng L, Liu J, Huang H. 2014.A simple method suitable to study de novo root organogenesis. Frontiers in Plant Science 5, 208.
Yarra R, Aileni M, Vemunoori AK, Kokkirala
VR, Umate P, Abbagani S. 2010. Direct shoot regeneration from mature leaf explants of Sphaeranthus indicus L., a multipurpose medicinal plant. Journal of Phytology 2, 05.
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