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Study of multidrug-resistant clinical isolates of Staphylococcus coagulase negative from Assir region of Saudi Arabia

By: N M Abdalla

Key Words: Coagulase-negative; Gram positive; Mec A gene, Multidrug resistance MDR, PCR.

Int. J. Biosci. 10(2), 156-165, February 2017.

DOI: http://dx.doi.org/10.12692/ijb/10.2.156-165

Abstract

Staphylococcus Coagulase negative bacteria are gram-positive and spherical cells in clusters. They commonly occur as skin commensals, occasionally cause nosocomial or community acquired infections. Numerous coagulase-negative staphylococci appear commonly on human skin, of these species, Staphylococcus epidermidis and S. hominis are the most abundant. While S. epidermidis tends to colonize the upper part of the body, S. hominis tends to colonize in areas with numerous apocrine glands, such as axillae and the pubic region. In this study, a total of 70 nasal swabs from isolates at Assir Central Hospital General Lab during the period of Sep. 2014- April 2015. The samples were tested by bactech, culture media, antibiotics sensitivity using diffusion disc test (MIC) and molecular polymerase chain reaction (PCR) for detection of the Mec A gene. Drugs found to be resistant to all patients were penicillin 100%, 60-70% to; erythromycin, ampicillin, cifoxine, carbinicillin, methotrexate and cefaclor. Drugs that showed resistance of 30-50% were; tetracyclin, fucidin, augmentin, gentamycin and ciprofloxacin. Whereas cotrimexazole and vancomycine were sensitive to all patients. All isolates were Mec A gene negative. In contrast to S. aureus, the coagulase-negative Staphylococci (CoNS) have been the subject of considerably less investigation, Normal commensals, however, CoNS species 15 are capable of causing infectious keratitis, sepsis of patients in neonatal intensive care units, and are now being recognized as a cause of gastroenteritis This study showed that, Staphylococcus coagulase negative bacteria were pathogen associated with community acquired and nosocomial infections. The frequency of staphylococcus multi-drugs resistance is rising.

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Study of multidrug-resistant clinical isolates of Staphylococcus coagulase negative from Assir region of Saudi Arabia

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N M Abdalla. 2017. Study of multidrug-resistant clinical isolates of Staphylococcus coagulase negative from Assir region of Saudi Arabia. Int. J. Biosci. 10(2), 156-165.
http://dx.doi.org/10.12692/ijb/10.2.156-165
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