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Significant correlation of MMTV (Mouse mammary tumor virus) LTR gene with hormone receptor status in peripheral blood samples of breast cancer patients from North Pakistan

By: Wasifa Naushad, Sana Ayub, Hajra Sadia

Key Words: Mouse mammary tumor virus, Breast cancer, MMTV-Ltr, Hormone receptors, ER/PR status

Int. J. Biosci. 10(3), 399-405, March 2017.

DOI: http://dx.doi.org/10.12692/ijb/10.3.399-405

Abstract

Mouse Mammary Tumor Virus (MMTV); a Beta retrovirus is the causative agent of mammary tumors in mice. Studies have suggested role of MMTV, as the contrasting factor that could cause Breast cancer in humans however, the exact mechanism of action is still to be explored. In this study, our aim was to investigate the association of MMTV-Ltr gene prevalence with hormone receptor status in breast cancer patients. A total of 50 peripheral blood samples of breast cancer patients were collected for this study. Detection of MMTV-Ltr sequences was done by end time PCR. Statistical analysis was carried out using SPSS 16.0 software. Molecular analysis of blood samples revealed the prevalence of MMTV-Ltr gene sequences in 19 of 50 (38%). In the current study we report the presence of MMTV LTR sequences in blood samples of breast cancer patients and their significant correlation with hormone receptor status of the breast cancer disease. A significant (p ≤ 0.05) correlation was found between the Estrogen receptor, her2/neu status and metastasis of the breast cancer patients with presence of MMTV-Ltr gene. No significant correlation was found in case of progesterone receptors and MMTV-Ltr gene presence in breast cancer samples. Presence of MMTV-Ltr and its correlation with hormones receptor status revealed the etiological involvement of MMTV in breast cancer patients. These results strengthen the role of MMTV as a hormone responsive virus and its role in causing Hormone positive breast cancer.

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Significant correlation of MMTV (Mouse mammary tumor virus) LTR gene with hormone receptor status in peripheral blood samples of breast cancer patients from North Pakistan

Buetti E, Diggelmann H. 1981. Cloned mouse mammary tumor virus DNA is biologically active in transfected mouse cells and its expression is stimulated by glucocorticoid hormones. Cell 23(2), 335-345.

DOI: 0092-8674(81)90129-X [pii].

Choi Y, Kappler JW, Marrack P. 1991. A superantigen encoded in the open reading frame of the 3′ long terminal repeat of mouse mammary tumour virus. Nature 350(6315), 203-207.

DOI: 10.1038/350203a0.

Craig Allred D, Clark G, Molina R, TANDON A, SCHNITT S, GILCHRIST K, MCGUIRE W. 1992. Overexpression of HER-2/neu and its relationship with oter prognostic factors change during the progression of in situ to invasive breast cancer. Human pathology 23(9), 974-979.

Faedo M, Ford CE, Mehta R, Blazek K. Rawlinson WD. 2004. Mouse mammary tumor-like virus is associated with p53 nuclear accumulation and progesterone receptor positivity but not estrogen positivity in human female breast cancer. Clin Cancer Res 10(13), 4417-4419.

DOI: 10.1158/1078-0432.CCR-03-0232.

Ford CE, Tran D, Deng Y, Ta VT, Rawlinson WD, Lawson JS. 2003. Mouse mammary tumor virus-like gene sequences in breast tumors of Australian and Vietnamese women. Clin Cancer Res 9(3), 1118-1120.

Fukuoka H, Moriuchi M, Yano H, Nagayasu T, Moriuchi H. 2008. No association of mouse mammary tumor virus-related retrovirus with Japanese cases of breast cancer. J Med Virol 80(8), 1447-1451.

DOI: 10.1002/jmv.21247.

Golovkina TV, Chervonsky A, Dudley JP, Ross SR. 1992. Transgenic mouse mammary tumor virus superantigen expression prevents viral infection. Cell 69(4), 637-645.

DOI: 0092-8674(92)90227-4 [pii].

Gunzburg WH, Salmons B. (1992). Factors controlling the expression of mouse mammary tumour virus. Biochem J, 283 ( Pt 3), 625-632.

Labrecque LG, Barnes DM, Fentiman IS, Griffin BE. 1995. Epstein-Barr virus in epithelial cell tumors: a breast cancer study. Cancer Res 55(1), 39-45.

Langerod A, Zhao H, Borgan O, Nesland JM, Bukholm IR, Ikdahl T, Jeffrey SS. 2007. TP53 mutation status and gene expression profiles are powerful prognostic markers of breast cancer. Breast Cancer Res 9(3), R30.

DOI: bcr1675.

Lawson JS, Gunzburg WH, Whitaker NJ. 2006. Viruses and human breast cancer. Future Microbiol 1(1), 33-51.

DOI: 10.2217/17460913.1.1.33.

Lawson JS, Tran DD, Carpenter E, Ford CE, Rawlinson WD, Whitaker NJ, Delprado W. 2006. Presence of mouse mammary tumour-like virus gene sequences may be associated with morphology of specific human breast cancer. J Clin Pathol 59(12), 1287-1292.

DOI: jcp.2005.035907.

Levine PH, Pogo BG, Klouj A, Coronel S, Woodson K, Melana SM, Holland JF. 2004. Increasing evidence for a human breast carcinoma virus with geographic differences. Cancer 101(4), 721-726.

DOI: 10.1002/cncr.20436.

Liu B, Wang Y, Melana SM, Pelisson I, Najfeld V, Holland JF, Pogo BG. 2001. Identification of a proviral structure in human breast cancer. Cancer Res 61(4), 1754-1759.

Mant C, Hodgson S, Hobday R, D’Arrigo C, Cason J. 2004. A viral aetiology for breast cancer: time to re-examine the postulate. Intervirology 47(1), 2-13.

DOI: 10.1159/000076636.

Melana SM, Nepomnaschy I, Sakalian M, Abbott A, Hasa J, Holland JF, Pogo BG. 2007. Characterization of viral particles isolated from primary cultures of human breast cancer cells. Cancer Res 67(18), 8960-8965.

DOI: 10.1158/0008-5472.CAN-06-3892.

Naushad W, Bin Rahat T, Gomez MK, Ashiq MT, Younas M, Sadia H. 2014. Detection and identification of mouse mammary tumor virus-like DNA sequences in blood and breast tissues of breast cancer patients. Tumour Biol 35(8), 8077-8086.

DOI: 10.1007/s13277-014-1972-3.

Park JW, Neve RM, Szollosi J, Benz CC. 2008. Unraveling the biologic and clinical complexities of HER2. Clinical breast cancer 8(5), 392-401.

Pucillo CE, Palmer LD, Hodes RJ. 1995. Superantigenic characteristics of mouse mammary tumor viruses play a critical role in susceptibility to infection in mice. Immunol Res 14(1), 58-68.

Ringold GM. 1979. Glucocorticoid regulation of mouse mammary tumor virus gene expression. Biochim Biophys Acta 560(4), 487-508.

Ross SR. 2000. Using genetics to probe host-virus interactions; the mouse mammary tumor virus model. Microbes Infect 2(10), 1215-1223.

DOI: S1286-4579(00)01275-2 [pii].

Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Erlich HA. (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239(4839), 487-491.

Salmons B, Gunzburg WH. 1987. Current perspectives in the biology of mouse mammary tumour virus. Virus Res 8(2), 81-102.

DOI: 0168-1702(87)90022-0.

Scheidereit C, Beato M. 1984. Contacts between hormone receptor and DNA double helix within a glucocorticoid regulatory element of mouse mammary tumor virus. Proc Natl Acad Sci U S A, 81(10), 3029-3033.

Taneja P, Frazier DP, Kendig RD, Maglic D, Sugiyama T, Kai F, Inoue, K. 2009. MMTV mouse models and the diagnostic values of MMTV-like sequences in human breast cancer. Expert Rev Mol Diagn 9(5), 423-440.

DOI: 10.1586/erm.09.31.

Wang Y, Go V, Holland JF, Melana SM, Pogo BG. 1998. Expression of mouse mammary tumor virus-like env gene sequences in human breast cancer. Clin Cancer Res 4(10), 2565-2568.

Wang Y, Melana SM, Baker B, Bleiweiss I, Fernandez-Cobo M, Mandeli JF, Pogo BG. 2003. High prevalence of MMTV-like env gene sequences in gestational breast cancer. Med Oncol 20(3), 233-236.

DOI: MO:20:3:233.

Wasifa Naushad, Sana Ayub, Hajra Sadia.
Significant correlation of MMTV (Mouse mammary tumor virus) LTR gene with hormone receptor status in peripheral blood samples of breast cancer patients from North Pakistan.
Int. J. Biosci. 10(3), 399-405, March 2017.
http://www.innspub.net/ijb/significant-correlation-mmtv-mouse-mammary-tumor-virus-ltr-gene-hormone-receptor-status-peripheral-blood-samples-breast-cancer-patients-north-pakistan/
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