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Serum IL-12p70 and TLR 7 gene expression in Egyptian patients infected with HCV and treated with Sofosbuvir, Ribavirin and/or Pegylated interferon

By: Hala M. Aldesouki, Hanan M. Zidan, Eman M. Elashry

Key Words: Hepatitis C virus, Ribavirin, Sofosbuvir, TLR7, IL-12

Int. J. Biosci. 10(6), 179-194, June 2017.

DOI: http://dx.doi.org/10.12692/ijb/10.6.179-194

Abstract

Chronic hepatitis C virus infection is one of the most important health problems in Egypt. The purpose of this study was to assess the efficacy, and tolerability of two therapeutic modalities, dual (Sofosbuvir+ribavirin)and triple (sofosbuvir + ribavirin + peg-interferon), in treating Egyptian patients infected with HCV genotype-4. In addition, we determined serum IL-12p70 and TLR7 gene expression in patients infected with HCV with and without treatment, compared to the normal volunteers. No significant changes in serum creatinine and fasting blood glucose concentrations were reported at the end of the treatment with dual and triple therapy and after 3 months of follow up. Pateints infected with HCV showed a non significant change in the relative quantitation of TLR7 gene expression, and a significant elevation in serum IL-12p70 concentration (161.73%, p<0.0001), compared to the volunteers group (II). In dual and triple therapy groups (III &IV), there was a significant elevation in the relative quantitation of TLR7 gene expression (250.53 and 506.32%, respectively, p<0.0001), compared with HCV group, but these elevations returned to the normal level after 3-months of follow up period. On the other hand, the concentration of serum IL-12p70 was significantly reduced in all treated groups, compared to both healthy volunteers and HCV groups , except group III (SOF+RBV) showed a non significant change compared to healthy volunteers. The treatment modalities, either with Sofosbuvir plus ribavirin or Sofosbuvir plus ribavirin and pegylated Interferon is highly effective in our cohort of Egyptian patients infected with HCV genotype-4. Further investigations should be done to increase the time after the end of treatment modalities to ensure complete remission of HCV infection.

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Serum IL-12p70 and TLR 7 gene expression in Egyptian patients infected with HCV and treated with Sofosbuvir, Ribavirin and/or Pegylated interferon

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Hala M. Aldesouki, Hanan M. Zidan, Eman M. Elashry. 2017. Serum IL-12p70 and TLR 7 gene expression in Egyptian patients infected with HCV and treated with Sofosbuvir, Ribavirin and/or Pegylated interferon. Int. J. Biosci. 10(6), 179-194.
http://dx.doi.org/10.12692/ijb/10.6.179-194
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