Screening of antibacterial activity of two tomato varieties against multi-drug resistant human pathogenic bacteria
By: Mst. Samima Nasrin, Fatema Jesmin, Sarwar Parvez, Mohammad Firoz Alam
Key Words: Tomato, Antibacterial activity, Pathogenic bacteria, MIC and MBC.
Int. J. Biosci. 8(2), 241-251, February 2016.
The present investigation deals with the test of antibacterial activity of fruit extracts of two tomato varieties viz., Raton and Persimmon against five Gram positive bacteria viz., Staphylococcus aureus, Bacillus cereus, Streptococcus-β-haemolytica, Bacillus subtilis, Sarcinaluteaand five Gram negative bacteria viz., Klebsiellasp., Klebsiella pneumonia, Pseudomonas aeruginosa, Salmonella typhi, Shigelladysenteriae. Between the two varieties, Raton and Persimmon in two different solvents (methanol and ethanol), ethanol fruit extracts of Raton have showed the highest zone of inhibition (19.50±0.28mm) against Gram negative bacteria Pseudomonas aeruginosa. MIC and MBC of the extracts are ranged from 100-300 mg/ml and 150-400 mg/ml respectively. The lowest MIC and MBC values are observed against Pseudomonas aeruginosa, Klebsiella sp. and Staphylococcus aureus. From the statistical results, it is seen that except replication, bacterial species, solvent and bacterial strain are significantly different with each other. In addition, interaction between bacterial species and solvent has showed that they are significantly different. The result indicates that the ethanol fruit extracts of tomato could be considered as a source of novel antibacterial agents against multi-drug resistant pathogenic bacteria.