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Screening and characterization of Rhizobacteria antagonistic to Pseudomonas syringae causing bacterial canker of stone fruits in Punjab and KPK

By: Shagufta Bibi, M. Inam-Ul-Haq, Abid Riaz, Saad Imran Malik, M. Ibrahim Tahir, Raees Ahmed

Key Words: Rhizobacteria, Pseudomonas syringae, Stone fruits, Bacterial canker, Screening

Int. J. Biosci. 10(5), 405-412, May 2017.

DOI: http://dx.doi.org/10.12692/ijb/10.5.405-412

Abstract

Several bacteria colonize plant roots but few of them promote plant growth directly or indirectly are termed as plant growth-promoting rhizobacteria (PGPR). Current study was conducted to isolate rhizobacterial isolates antagonistic to Pseudomonas syringae causing bacterial canker of the stone fruits(apricot, peach and plum) growing in the areas of Punjab and Khyber Pakhtoonkhwa (KPK) provinces of Pakistan. A total of 80 (later pooled to get 16) soil samples were collected from the rhizosphere of apricot, peach and plum and 185 isolates were obtained. Out of 185 rhizobacterial isolates, 10 isolates showed greater potential (> 20 mm zone of inhibition) during in vitro antagonistic activity against P. syringae, and were selected for their morphological and biochemical characterization. Among antagonistic isolates, 6 responded negatively and 4 positively to Gram’s straining. All the antagonistic isolates possessed one or more growth promoting character(s) such as siderophore production, phosphate solubilization, ammonia production and hydrogen cyanide (HCN) production. Six isolates were positive to fluorescence production. Enzymatic activity was exhibited by 8 isolates, during their catalase and lipase activity. Therefore present study suggested that isolates having antagonistic potential as well as plant growth promoting characteristics can be used as bio-protectant/bio-fertilizer to enhance plant growth in stone fruits.

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Screening and characterization of Rhizobacteria antagonistic to Pseudomonas syringae causing bacterial canker of stone fruits in Punjab and KPK

Bakker AW, Schipperes B. 1987. Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp. – mediated plant growth stimulation. Soil Biology and Biochemistry 19(4), 451-457.

Bhattacharyya PN, Jha DK. 2012. Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World Journal of Microbiology and Biotechnology 28, 1327-1350.

Cappuccino JC, Sherman N. 1992. A Laboratory Manual, 3 Edn, Benjamin/cummings Pub. Co. New York. Microbiology 125-179.

Cassan F, Maiale S, Masciarelli O, Vidal A, Luna V, Ruiz O. 2009. Cadaverine production by Azospirillum brasilense and its possible role in plant growth promotion and osmotic stress mitigation. European Journal of Soil Biology 45, 12-19.

Gilbert V, Legros F, Maraite H, Bultreys A. 2009. Genetic analyses of Pseudomonas syringae isolates from Belgian fruit orchards reveal genetic variability and isolate-host relationships within the pathovar syringae, and help identify both races of the pathovar morsprunorum. European Journalof Plant Pathology 124, 199-218.

György É, Mara GY, Máthé I, Laslo É, Márialigeti K, Albert B, Oancea F, Lányi SZ. 2010. Characterization and diversity of the nitrogen fixing microbiota from a specific grassland habitat in the Ciuc Mountains. Romanian Biotechnological Letters 15, 5474-5480.

Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. 1994. In: Bergey’s Manual of Determinative Bacteriology. 9th ed. Williams & Wilkins, Baltimore MD. 184.

Husen E. 2003. Screening of soil bacteria for plant growth promoting activities in vitro. Indones. Journal of Agricultural Sciences 4, 27-31.

Kim HS, Park J, Choi SW, Choi KH, Lee GP, Ban SJ, Lee HL, Kim CS. 2003. Isolation and characterization of Bacillus strains for biological control. The Journal of Microbiology 41, 196-201.

Krey T, Vassilev N, Baum C, Eichler- Löbermann B. 2013. Effects of longterm  phosphorus application and plant growth promoting rhizobacteria on maize phosphorus nutrition under field conditions. European Journal of Soil Biology 55, 1124-1130.

Leong J. 1986. Siderophores: Their biochemistry and possible role in the biocontrol of plant pathogens. Annual Review of Phytopathology 24, 187-209.

Loper JE, Schroth MN. 1986. Influence of bacterial sources of indole-2-acetic acid on root elongation of sugar beet. Phytopathology 76, 386-389.

Mohammadi M, Ghasemi A, Rahimian H. 2010. Phenotypic Characterization of Iranian Strains of Pseudomonas syringae pv. syringae van Hall, the Causal Agent of Bacterial Canker Disease of Stone Fruit Trees. Journal of Agricultural Science and Technology 3, 51-65.

Neeru N, Vivek K, Rishi K, Wolfgancy M. 2000. Effect of P-solubilizing Azotobacter chroococcum on N, P, K uptake in p-responsive genotypes grown under greenhouse condition. Journal of Plant Nutrition and Soil Science 163, 393-398.

Nelson LM. 2004. Plant growth promoting rhizobacteria (PGPR): Prospects for new inoculants. Crop Management.

Omidvari M. 2008. Biological control of Fusarium solani, the causal agent of damping off, by fluorescent pseudomonads and studying some of their antifungal metabolite productions on it. MS thesis, Tehran University Iran.

Pikovaskya RE. 1948. Mobilization of phosphorous in soil in connection with vital activity of some microbial species: Mikrobiologiya 17, 362-370.

Rajkumar M, Ae N, Prasad MNV, Freitas H. 2010. Potential of siderophore-producing bacteria for improving heavy metal phytoextraction. Trends in Biotechnology 28, 142-149.

Rajkumar M, MA Y, Freitas H. 2008. Characterization of metal resistant plant-growth promoting Bacillus weihenstephanensis isolated from serpentine soil in Portugal. Journal of Basic Microbiology 48, 500-508.

Rana A, Saharan B, Joshi M, Prasanna R, Kumar K, Nain, L. 2011. Identification of multi-trait PGPR isolates and evaluating their potential as inoculants for wheat. Annals of Microbiology 61, 893-900.

Rosenblueth M, Martinez-Romero E. 2006. Bacterial endophytes and their interactions with hosts. Molecular Plant Microbe Interaction 19, 827- 837.

Sinha L, Simon S. 2013. Biochemical, morphological and functional characterization of Pseudomonas Fluorescents against Alter aria porri of aloe Vera. Journal of Research 25(1), 68-73.

Teaumroong N, Wanapu C, Chankum Y, Arjharn W, Sang-Arthit S, Teaimthaisong K, Boonkerd N. 2010. Production and application of bioorganic fertilizers for organic farming systems in Thailand: A Case Study 293-312.

Verma JP, Yadav J, Tiwari KN, Kumar A. 2013. Effect of indigenous Mesorhizobium spp. and plant growth promoting rhizobacteria on yields and nutrients uptake of chickpea (Cicer aritenium L.) under sustainable agriculture. Ecological Engineering 51, 282-286.

Walker TS, Bais HP, Grotewold E, Vivanco JM. 2003. Root exudation and rhizosphere biology. Plant Physiology 132, 44-51.

Wang S, Huijun W, Junqing Q, Lingli M, Jun L, Yanfei X, Xuewen G. 2009. Molecular mechanism of plant growth promotion and induced systemic resistance to tobacco mosaic virus by Bacillus spp. Journal of Microbiology and Biotechnology 19(10), 1250-1258.

Weller DM, Raaijmakers JM, Mcspadden BB, Thomashow LS. 2002. Microbial populations responsible for specific soil suppressive ness to plant pathogens. Annual Review of Phytopathology 40, 309-348.

Weyens N, Van der Lelie D, Taghavi S, Newman L, Vangronsveld J. 2009. Exploiting plantemicrobe partnerships to improve biomass production and remediation. Trends in Biotechnology 27, 591-598.

Wollum AG. 1982. Methods of soil analysis. Medison Wisconsin, USA 781-801.

Xie H, Pasternak JJ, Glick BR. 1996. Isolation and characterization of mutants of the plant growth-promoting rhizobacterium Pseudomonas putida GR12-2 that overproduce indoleacetic acid. Current Microbiology 32, 67-71.

Zaidi A, Khan MS, Ahemad M, Oves M. 2009. Plant growth promotion by phosphate solubilizing bacteria. Acta Microbiological et Immunological Hungarica 56, 263-280.

Shagufta Bibi, M. Inam-Ul-Haq, Abid Riaz, Saad Imran Malik, M. Ibrahim Tahir, Raees Ahmed.
Screening and characterization of Rhizobacteria antagonistic to Pseudomonas syringae causing bacterial canker of stone fruits in Punjab and KPK.
Int. J. Biosci. 10(5), 405-412, May 2017.
http://www.innspub.net/ijb/screening-characterization-rhizobacteria-antagonistic-pseudomonas-syringae-causing-bacterial-canker-stone-fruits-punjab-kpk/
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