International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Screening an antifungal efficacy of indigenous bacteria from rhizosphere of Periploca apylla

By: Qais Ahmad Naseer, Kalsoom, Johar Jamil, Haji Khan, Fazal Jalil

Key Words: Rhizosphere, Periploca aphylla, Swat, Gogdara hill, Bacterial strains

Int. J. Biosci. 11(1), 351-359, July 2017.

DOI: http://dx.doi.org/10.12692/ijb/11.1.351-359

Certification: ijb 2017 0039

Certificate
Cover

Abstract

The Periploca aphylla is an important medicinal plant helps in surmounting constipation and curing swollen joints. Bacterial isolates were screened from rhizosphere of Periploca apylla for antifungal efficacy against Alternara pane, Colletotrichum cocealesx, and Rhizocotina solani, Gogdara Hill-Odigram, Swat Pakistan. Isolates were cultured on nutrient agar and identified through gram staining and biochemical tests. Lipolytic activity, salinity, temperature ranges, antibiotic sensitivity and antifungal efficacy were also studied. The results of cultural and morphological study revealed that all bacterial isolates were gram negative except PaS2. Biochemical analysis showed that all Isolates were positive for Ortho-Nitro Phenyl-ß-D-Galacto-pyranosidase, glucose fermentation, arabinose fermentation, ornithine Decarboxylase, citrate utilization and negative for lysine decarboxylase, H2S production, urease production and indole production (IND) test. Based on result of cultural and biochemical analysis PaS1 was classified under the genus of Phenylobacterium, PaS2, PaS3 and PaS4 were suspected to be Bacillus lentus, Entrerobacter aerogene and Aeromonas caviae respectively. All strains were able to tolerate different salt concentration ( 3%, 1%, 0.5%) except PaS1 which showed growth at 0.5 %, whereas maximum growth for all isolates were observed at 30°C to 37°C.The bacterial isolates sensitive to different concentrations of ampicillin, kanamycin and streptomycin. Antifungal efficacy indicated that all fungal strains were resistant to PaS3 and sensitive to PaS4, whereas only Alternara panex were sensitive to PaS1 and PaS2. It is concluded that PaS4, PaS1 and PaS2 can be used as a bioinoculant against fungal pathogens. The findings will be helpful for the production of useful antifungal substances at commercial level.

| Views 30 |

Screening an antifungal efficacy of indigenous bacteria from rhizosphere of Periploca apylla

Badshah L, Hussain F. 2011. People preferences and use of local medicinal flora in District Tank, Pakistan. Journal of Medicinal Plants Research 5, 122-29. ISSN 1996-0875 ©2011 Academic Journals.

Bais HP, Prithiviraj B, Jha AK, Ausubel FM, Vivanco JM. 2006 The role of root exudates in rhizosphere interactions with plants and other organisms. Annual Review of Plant Biology 57, 233–266.

Beutner KR, Krog V. 1990. Current status of podophyllotoxin for the treatment of warts. Seminars in Dermatology 9, 148.

Blasko G, Cordell GA.1998. Recent Developments in Chemistry of Plant Derived Anticancer Agents. Economics and Medicinal Plant Research, Academics Press, London 1, 113-153.

Canel C, Moraes F, Dayan, Ferreria D. 2000. Podophyllotoxin. Phytochemistry 54,115-120.

Nazir T, Uniyal AK, Todaria NP. 2006. Allelopathic behavior medicinal plants pecies on traditional agriculture crops Himalaya, India. Agroforestry system journal 69(3), 183-187.

Mahmood A, Tabassum A. 2012. Ethnobo- tanical Survey of Plants from District Sialkot. Pakistan Journal of Applied Pharmacy 3, 2220.

Murad W, Ahmad A, Gilani SA, Khan MA. 2011. Indigenous Knowledge and Folk Use of Medicinal Plants by the Tribal Communities of Hazar Nao Forest, Malak- and District, North Pakistan. Journal of Medicinal Plants Research 5, 1072-1086.

Musliu N, Martin, Schwengerer. 2013. Algorithm Selection for the Graph Coloring Problem. Springer. In LION, vol 7997 of LNCS, pages 389–403.

Roshetko JM, Lasco RD, Delos Angeles MD. 2007. Small holder agro-forestry systems for carbon storage. Mitigation Adapt Strategies Global Change 12, 219–242.

Shamima N, Rahman MA. 2007. International Journal of Biological sciences 15, 77-82.

Shubhrasekhar C, Supriya M, Karthik L,

Gaurav K, Bhaskara Rao KV. 2013. Isolation, characterization, and application of biosurfactant produced by marine action bacteria isolated from saltpan soil from costal area of Andhra Pradesh, India. Research Journal of Biotechnology 8(1), 18.

Sumathi C, Jayashree, Sekaran G. 2012. Screening and evaluation of antimicrobial activity of tannery fleshing utilizing fish gut bacteria. International Journal of Pharmacology 8, 373-381.

Tangjang S, Arunachalam K, Arunachalam A, Shukla AK. 2009. Microbial population         dynamics of soil under traditional agroforestry systems in Northeast India. Res Journal of Soil Biology 1, 1–7.

Warcup JH. 1950. Basic plant pathology methods. Nature London 166, 117.

Web. References

Swat valley.

http://www.swatfruit.com/index.php/en/swat-valley/swat-valley

Qais Ahmad Naseer, Kalsoom, Johar Jamil, Haji Khan, Fazal Jalil.
Screening an antifungal efficacy of indigenous bacteria from rhizosphere of Periploca apylla.
Int. J. Biosci. 11(1), 351-359, July 2017.
http://www.innspub.net/ijb/screening-antifungal-efficacy-indigenous-bacteria-rhizosphere-periploca-apylla/
Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
http://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update