International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Safety assessment of the fruit extract of Acacia nilotica Linn. Willd ex Delile using rat models

By: Juliette Koubé, Sélestin Sokeng Dongmo, Valentin Désiré Guiama, Aïssatou Doubla, Jeweldai Vedekoi, Michelle Goulimé, Elizabeth Ngo Bum

Key Words: Acacia nilotica fruit, Aqueous extract, Oral acute and subchronic toxicities, Safety

Int. J. Biosci. 10(4), 81-92, April 2017

DOI: http://dx.doi.org/10.12692/ijb/10.4.81-92

Abstract

The Acacia nilotica fruits are commonly used for their medicinal and food merits. The present work was carried out to evaluate the innocuousness of an Acacia nilotica aqueous fruit extract (ANFE) using acute and sub-chronic oral administration in rats. ANFE doses of 1, 2, 4, 8 and 16 g/kg were orally administered to rats, the mortality and toxic effects were observed for 14 days. For sub-chronic test, ANFE doses of 200, 400, 800 mg/kg and satellite group (800 mg/kg) were orally given to rats daily for 35 days, control was distilled water. Food behaviour, body weight, food efficiency and weight of faeces were followed during the treatment. At the end of administration, all the rats were kept fasted overnight and then sacrificed under anaesthesia. Blood was then collected from jugular vein for haematological and biochemical analyses. Results show that a single dose of ANFEs oral administration did not cause any mortality; LD50 was > 16 g/kg. Daily oral administration of ANFE increased significantly the food and water consumption, but decreased the faeces weight after 2 weeks of treatment. However, no significant modifications were observed in the haematological values, markers of renal and hepatic functions, body weight, absolute and relative organ weights, structures of kidney and liver. There were remarked hypoglycaemic, hypocholesterolaemic and hypolipidaemic effects of ANFE. These results suggest that oral administration of ANFE does not induce oral acute and sub-chronic toxicities. Therefore, these safety levels of A. nilotica fruit aqueous extract can be helpful for its optimal utilisation.

| Views 48 |

Safety assessment of the fruit extract of Acacia nilotica Linn. Willd ex Delile using rat models

Al-Mustafa ZH, Dafallah AA. 2000. A study on the toxicology of Acacia nilotica. American Journal of Chinese Medicine 28, 123 – 129.

http://dx.doi.org/10.1142/S0192415X00000155

Arbonnier M. 2000. Arbres, arbustes et lianes des zones sèches d’Afrique de l’Ouest. CIRAD-MNHN-UICN, France, 541 p.

Bachaya HA, Iqbal Z, Khan MN, Sindhu Z, Jabbar A. 2009. Anthelmintic activity of Ziziphus nummularia (bark) and Acacia nilotica (fruit) against Trichotronylid nematodes of sheep. Journal of Ethnopharmacology 123, 325 – 329.

http://dx.doi.org/10.1016/j.jep.2009.02.043.

El-Hadiyah TM, Abdulhadi NH, Badico, EEM, Mohammed EYG. 2011. Toxic potential of ethanolic extract of Acacia nilotica (Garad) in rats. Sudanese Journal of Medicinal Sciences 6, 1 – 6.

http://dx.doi.org/10.4314/sjms.v6i1.67269

El-Tahir A, Satti GM, Khalid SA. 1999. Antiplasmodial activity of selected Sudanese medicinal plants with emphasis on Acacia nilotica. Phototherapy Research 13, 474 – 478.

Gilani AH, Shaheen F, Zaman M, Janbaz KH, Shah BH, Akhtar MS. 1999. Studies on antihypertensive and antispasmodic Activities of Methanol Extract of Acacia nilotica pods. Phytotherapy Research 13, 665–669.

Guta M, Urga K, Assefa A, Lemma H, Addis G, Gemeda N, Yirsaw K, Mudi K, Melaku D. 2007. Antibacterial and acute toxicity study of Acacia nilotica. Ethiopian journal of Biological Sciences 6, 43 – 49.

http://dx.doi.org/10.4314/ejbs.v6i1.39039

ILAR. 2011. Guide for the care and use of laboratory animals. Committee for the update of the guide for the care and use of laboratory animals, 8th edition. Washington, United States of America. 209 p.

Koubé J, Sokeng Dongmo S, Guiama VD, Ngo Bum E. 2016. Ethnomedicinal survey of Gavdé (Acacia nilotica): a medicinal plant used in sahelian zone of Cameroon, Central Africa. International Journal of Innovation and Applied Studies 16, 820 – 827.

Malviya S, Rawat S, Kharia A, Verma M. 2011. Medicinal attributes of Acacia nilotica Linn. – A comprehensive review on ethno pharmacological claims. International Journal of Pharmacy and Life Sciences 2, 830-837.

Sanni S, Thilza IB, Talle M. 2010. The effect of Acacia nilotica pod Ethyl Acetate fraction on induced diarrhoea in albino rats. New York Science Journal 3, 16-20.

Singh BN, Singh BR., Singh RL. 2009. Antioxidant and anti-quorum sensing activities of green pod of Acacia nilotica L. Food Chemical and Toxicology 47, 778–786.

http://dx.doi.org/10.1016/j.fct.2009.01.009.

Sokeng SD, Koubé J, Dongmo F, Sonnhaffouo S, Nkono Ya Nkono BL, Taïwé GS, Cherrah Y, Kamtchouing P. 2013. Acute and chronic anti-inflammatory effects of the aqueous extract of Acacia nilotica (L.) Del. (Fabaceae) pods. Academia Journal of Medicinal Plants 1, 001-005.

http://dx.doi.org/10.15413/ajmp.2012.0102

Wakte PS, SachinBS, Patil AA, Shinde DB. 2012. Hepatoprotective activity of Acacia nilotica flowers. Medicinal Chemistry and Drug Discovery 3, 152 – 159.

World Health Organization (WHO). 2000. General guidelines for methodologies on research and evaluation of traditional medicine. Switzerland.

Juliette Koubé, Sélestin Sokeng Dongmo, Valentin Désiré Guiama, Aïssatou Doubla, Jeweldai Vedekoi, Michelle Goulimé, Elizabeth Ngo Bum. 2017. Safety assessment of the fruit extract of Acacia nilotica Linn. Willd ex Delile using rat models. Int. J. Biosci. 10(4), 81-92.
http://dx.doi.org/10.12692/ijb/10.4.81-92
Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
http://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update