Protocol on direct organogenesis in Chrysanthemum morifolium Ramat
By: Rawnak Jahan Swarna, Dilruba Yeasmin, Md. Mostafizur Rahman, Md. Firoz Alam
Key Words: Chrysanthemum morifolium, nodal segments, direct organogenesis.
Int. J. Biosci. 9(4), 123-137, October 2016.
An efficient direct organogenesis protocol was developed for Chrysanthemum morifoliumRamat., an ornamental herb.The purpose of this study was todetermine the effect of different growth regulators on shoot formation and multiplication over the cultural periodand mass propagation in large quantities in a short timeusing tissue culture techniques.Nodal segments, after being sterilized with 0.1% HgCl2(mercuric chloride) for eight minutes, were inoculated in MS media (Murashige and Skoog, 1962)with different concentrations of BAP(6-benzyl amino purine) singly and combinations with KIN (6-furfural amino purine), Casein hydrolysate (CH), NAA (α-napthalene acetic acid). All of that, shoot growth and development showed better performancein 1.0 mg/l BAP singly, and 2.0 mg/l BAP with 0.5 mg/l KIN.In vitrorooting was successfully achieved on MS media supplemented with different concentrations of auxins.Better rooting response was obtained in MS media supplemented with 2.0 mg/l and 2.5 mg/l IBA(Indole-3 butyric acid) and 0.5 mg/l IBA with 1.5 mg/l NAA, respectively. The results from the study will enhance rapid multiplication of high quality planting materials ofChrysanthemum morifoliumand other valuable plants.