Pathogenesis and molecular confirmation of Pseudomonas Syringae PV. Syrinage Isolates from peach and plum in Pakistan
By: Raees Ahmed, Muhammad Inam-ul-Haq, Muhammad Ashfaq, Zahid Akram
Key Words: Pseudomonas syringae pv. syringae, virD4, Stone fruits, Pathogenesis.
Int. J. Biosci. 8(5), 22-28, May 2016.
Detailed survey of Peach and Plum orchards was done in 2014-2015 for the confirmation of bacterial canker disease in Pakistan and the isolates were isolated from diseased stone fruit tissue samples from 16 Orchard sites in Khyber Pakhtunkhwa (KPK), Pakistan. These isolates were tested for pathogenicity and the confirmation of Pseudomonas syringae pv. syringae was done using virD4 primer for the amplification of virD4 gene. All the 21 isolates from 24 isolates tested were moderately to highly pathogenic on peach seedlings while other 3 isolates showed no pathogenicity. During PCR assay of all the 24 isolates to amplify virD4 gene, the predicted 1453bp PCR products were obtained by 20 isolates and the 4 isolates were considered negative. The results highlighted a new threat in Pakistan, which must be further studied for its characterization and epidemiology for better management of bacterial canker of stone fruits in local environmental conditions.