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Partial characterization of exopolysaccharides produced by Leuconostoc sp. Isolated from intestine of Animals

By: S. Larouci, A. Guermouche, F. Chaib, F. Bensalah

Key Words: Exo polysaccharides, Production, Leuconostoc sp., DNA

Int. J. Biosci. 10(6), 208-215, June 2017.

DOI: http://dx.doi.org/10.12692/ijb/10.6.208-215

Abstract

Exopolysaccharides (EPS) produced by lactic acid bacteria (LAB) have gained increasing attention over the last few years because of their contribution to the rheology and texture of food products. For this purpose, a total of twelve LAB strains were isolated from intestinal content of coq and shrimp. Using a red ruthenium containing milk agar, four exopolysaccharides producing strains were selected and identified as Leuconostoc sp. on the basis of morphological characteristics and molecular identification using a genus specific primer which amplified an DNA fragment of 1200 pb. For EPS production and preparation, a selective sucrose media was used and show a highly viscous growth. Other work including the precipitation, hydrolysis of EPS and estimation of a total carbohydrate using a phenol sulfuric method were determined. The EPS production was varied from 654mg/l to 1254mg/l for the highest production for LGM 14 strain. Componential analysis of hydrolyzed EPS by thin layer chromatography indicated that it is a dextran, consisting of glucose monomer. The isolated strains of Leuconostoc sp. are a potent producer of dextran, which find its applications in various industries; it can be used as thickening or gelling agent in food.

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Partial characterization of exopolysaccharides produced by Leuconostoc sp. Isolated from intestine of Animals

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S. Larouci, A. Guermouche, F. Chaib, F. Bensalah.
Partial characterization of exopolysaccharides produced by Leuconostoc sp. Isolated from intestine of Animals.
Int. J. Biosci. 10(6), 208-215, June 2017.
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