International network for natural sciences – research journal
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Optimization, purification and characterization of invertase by Pseudomonas sp, isolated from the cane molasse

By: Zouaoui Benattouche, Ghalem Bachir Raho, Fatima Sahnouni, Djillali Bouhadi

Key Words: Invertase, Pseudomonas, Optimization, Purification, Cane molasse.

Int. J. Biosci. 9(1), 349-356, July 2016.

DOI: http://dx.doi.org/10.12692/ijb/9.1.349-356

Abstract

A Pseudomonas sp was isolated from cane molasse sample. The effect of different production parameters such as pH, temperature, incubation time, carbon source, nitrogen source (organic and inorganic), on invertase production by the isolated Pseudomonas sp strain were studied. The enzyme production was assayed in submerged fermentation (SmF). Maximum invertase activity was found at pH 6, 40°C, 48 hours, sucrose, yeast extract. The enzyme was purified by ammonium sulfate precipitation, dialysis and DEAE cellulose column chromatography. A trial for the purification of invertase resulted in an enzyme with specific activity of 39.56, with 25.7% recovery of invertase. More over this study gives us a hint of microbial wealth of invertase production which can be harnessed for biotechnological processes.

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