International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Optimization of inorganic phosphate solubilization by Pseudomonas fluorescens and Bacillus sp. isolated from wheat rhizospheric soil

By: Chibani Hiba Rahman, Bellahcene Miloud, Djibaoui Rachid, Bouznad Ahcene, Hamoum Hakim

Key Words: Bacillus sp., Phosphate solubilizing bacteria, Pseudomonas fluorescens, Optimization, Tricalcium phosphate

Int. J. Biosci. 10(4), 142-150, April 2017.



Phosphate solubilizing bacteria play a vital role in soil fertility. They are used to promote the growth of a large group of plants. The aim of this work was to evaluate the bacterial isolates’ capacity to release inorganic phosphate and optimizing their solubilization. Different culture media, nitrogen source and carbon source were used under varying culture conditions for optimizing solubilization of phosphate by two bacteria isolated from saline soil Pseudomonas fluorescens and Bacillus sp. Optimization of growth conditions was also tested using different incubation periods, temperature and pH. The comparison of amounts of phosphorus released by the isolates in different liquid cultures showed that the best solubilization was obtained in NBRIP medium for both Pseudomonas and Bacillus with quantities of 65.619 µg/mL and 560.667 µg/mL of free phosphorus, respectively. Glucose was found to be the best source of carbon for solubilization of phosphate by the two isolates. The effect of the variation of nitrogen source in the medium allowed to select ammonium sulfatas the most favorable nitrogen source for both bacterial isolates. The results showed that the pH = 5 and the incubation temperature of 30°C are optimal conditions for phosphate solubilization bybacterial isolates. The study of the effect of incubation time led to select the 6th day of incubation as an optimal time for phosphate solubilization by the two isolates.

| Views 39 |

Optimization of inorganic phosphate solubilization by Pseudomonas fluorescens and Bacillus sp. isolated from wheat rhizospheric soil

Ahuja A, Ghosh SB, D’souza SF. 2007.Isolation of a starch utilizing, phosphate solubilizing fungus on buffered medium and its characterization. Bioresource Technology 98, 3408-3411.

Barber SA. 1995. Soil nutrient bioavailability: A mechanistic approach, 2nd Edition, John Wiely, New York, USA.

Borling K, Otabbong E, Barberis E. 2001. Phosphorus sorption in relation to soil properties in some cultivated Swedish soils. Nutrient Cycling in Agroecosystems 59, 39-46.

Chen YP, Rekha PD, Arun AB, Shen FT. 2005. Phosphate-solubilizing bacteria from subtropical soil and their tricalcium phosphate-solubilizing abilities. Applied Soil Ecology 34, 33–41. 1016/ j. apsoil. 2005. 12. 002.

Cherif-Silini H, Silini A, Ghoul M, Yahiaoui B, Arif F. 2013.Solubilization of phosphate by the Bacillus under salt stress and in the presence of osmoprotectant compounds. African Journal of Microbiology Research 7, 4562-4571.

Fasim F, Ahmed N, Parsons R, Gadd GM. 2002.

Solubilization of Zinc salts by bacterium isolated by the air environment of tannery. FEMS Microbiology Letters 213, 1-6.

Goldstein AH. 1986. Bacterial solubilization of mineral phosphates: historical perspective and future prospects. American Journal of Alternative Agriculture 1, 51-57.

Halder AK, Mishra AK, Bhattacharya P,Chakrabarthy PK. 1990. Solubilization of rock phosphate by Rhizobium and Bradyrhizobium. Journal of General and Applied Microbiology 36, 81–92.

Illmer P, Schinner F. 1992.Solubilization of inorganic phosphates by microorganisms isolated from forest soil. Soil Biology and Biochemistry 24, 389-395.

Illmer P, Schinner F. 1995. Solubilization of inorganic calcium phosphates-solubilization mechanisms. Soil Biology and Biochemistry 27, 257-263.

Jackson ML. 1973.Soil chemical Analysis.Prentice Hall of Englewood cliffs, New Jersey, USA.

Johri JK, Surange S, Nautiyal CS. 1999. Occurrence of salt, pH, and temperature-tolerant, phosphate-solubilizing bacteria in alkaline soils. Current Microbiology 39, 89-93.

Khan MS, Zaidi A, Wani PA. 2007.Role of phosphate-solubilizing microorganisms in sustainable agriculture – A review. Agronomy for Sustainable Development, Springer Verlag 27, 29-43.

Khan MS, Ahmad E, Zaidi A, Oves M. 2013.

Functional aspect of phosphate-solubilizing crop productivity. bacteria: Importance in crop production. In: Maheshwari DK, Ed. Bacteria in agrobiology: Springer, Berlin, 237–265 P.

Khan MS, Zaidi A, Ahmad E. 2014. Mechanism of phosphate solubilisation and physiological functions of phosphate-solubilizing microorganisms. In: Khan MS, Zaidi A, Mussarrat, J, Eds. Phosphate Solubilizing Microorganisms., Springer International Publishing, Switzerland, 34-45 P.

Kucey RMN, Janzen HH, Legget ME. 1989. Microbial mediated increases in plant available phosphorus. Advances in Agronomy42, 199-228.

Kumar GK, Ram MR. 2014. Phosphate solubilizing rhizobia isolated from Vignatrilobata. American Journal of Microbiological Research 2, 105-109.

Lin TF, Huang HI, Shen FT, Young CC. 2006. The protons of gluconic acid are the major factor responsible for the dissolution of tricalcium phosphate by Burkholderia cepacia CC-Al74. Bioresource Technology 97, 957–960.

Lins MDCR, Fontes JEM, de Vasconcelos NM, da Silva Santos DM, Ferreira, OE, de Azevedo JLU, de Souza Lima GAM. 2014. Plant growth promoting potential of endophytic bacteria isolated from cashew leaves. African Journal of Biotechnology 13, 3360-3365.

Muleta D, Fassil A, Elisabe B, Granhall U. 2012. Phosphate-solubilisingrhizo bacteria associated with Coffea arabica L. in natural coffee forests of southwestern Ethiopia. Journal of the Saudi Society of Agricultural Sciences 12, 73-84.

Musarrat J,  Khan MS. 2014. Factors affecting  phosphate-solubilizing activity of microbes: current status. In Phosphate Solubilizing Microorganisms (p.

63-85). Springer International Publishing.

Nahas E. 1996. Factors determining rock phosphate solubilization by microorganism isolated from soil. The World Journal of Microbiology and Biotechnology 12, 18-23.

Nautiyal CS. 1999. An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiology Letters 170,265-270.

Nautiyal CS, Bhadauria S, Kumar P, Lal H, Mondal R, Verm D. 2000. Stress induced phosphate solubilization in bacteria isolated from alkaline soils. FEMS Microbiology Letters182, 291-296.

Pikovskaya RI. 1948. Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Microbiologia 17, 362-370.

Rodríguez H, Fraga R. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances 17, 319-339.

Shahab S,  Ahmed N. 2008.Effect of various parameters on the efficiency of zinc phosphate solubilization by indigenous bacterial isolates. African Journal of Biotechnolog y7, 10-25.

Shenoy VV, Kalagudi GM. 2005. Enhancing plant phosphorus use efficiency for sustainable cropping. Biotechnology Advances23, 501-513. 10.1016/j.biotechadv.2005.

Varsha-Narsian J, Thakkar J, Patel HH. 1994. Inorganic phosphate solubilization by some yeast. Indian Journal Microbiology 35, 113-118.

Wang X, Wang Y, Tian J, Lim BL, Yan X, Liao

  1. 2009. Overexpressing AtPAP15 enhances phosphorus efficiency in soybean. Plant Physiology 151, 233-240.

http:/ / dx. doi. org/10. 1104/ pp. 109.

Wani PA, Khan MS, Zaidi A. 2007. Synergistic effects of the inoculation with nitrogen fixing and phosphate-solubilizing rhizobacteria on the performance of field grown chickpea. Journal of Plant Nutrition and Soil Science 170, 283-287.

http:/ / dx. doi. org/10.1002/jpln.200620602.

Wenzel CL, Ashford AE, Summerell BA. 1994. Phosphate-solubilizing bacteria associated with proteoid roots of seedlings of waratah [Telopea speciosissima (Sm) R.Br.]. New Phytol 128, 487–496.

Xiao CQ, Chi RA, He H, Zhang WX. 2009. Characterization of tricalcium phosphate solubilization by Stenotrophomonas maltophilia YC isolated from phosphate mines. Journal of Central South University of Technology 16, 581-587.

Yadav K, Singh T. 1991. Phosphorus solubilization of microbial isolate from a calcifluvent. Journal of the Indian Society of Soil Science 39, 89-93.

Zhou K, Binkley D, Doxtader KG. 1992.A new method estimating gross phosphorus mineralization and immobilisation rates in soils. Plant and Soil 147, 243-250.

Chibani Hiba Rahman, Bellahcene Miloud, Djibaoui Rachid, Bouznad Ahcene, Hamoum Hakim.
Optimization of inorganic phosphate solubilization by Pseudomonas fluorescens and Bacillus sp. isolated from wheat rhizospheric soil.
Int. J. Biosci. 10(4), 142-150, April 2017.
Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
innspub logo
english language editing
    Publish Your Article
    Submit Your Article
Email Update