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Morphological characteristics and electrophoretic profiling is a primary tool to investigate Nickel (Ni) stress tolerance in Cenchrus ciliaris: A Cholistan desert grass

By: Asma Yaqoob, Faizul Hassan Nasim, Muhammad Azhar Zia

Key Words: Asma Yaqoob, Faizul Hassan Nasim, Muhammad Azhar Zia

Int. J. Biosci. 10(6), 232-245, June 2017.

DOI: http://dx.doi.org/10.12692/ijb/10.6.232-245

Abstract

Abiotic stresses are one of important ecological factors that disturb the normal process of growth and development in plants. Our environment is going to change rapidly so it is very important to protect plants from such ecological hazards. The first effect of these stresses is alteration in morphology of a plant. Secondly proteins metabolism of plants are also affected very rapidly after exposing them to any kind of stress. Theses morphological and protein changes are important to assess the tolerance capabilities of a plant and also give a clue about the types of internal changes that occur in plants. There are many kinds of abiotic stresses and heavy metal stress is one of them. Heavy metals are important micronutrients but their excess amounts are toxic for plants. Cenchrus ciliaris a perennial grass from Cholistan desert was exposed to Nickle metal stress in different concentrations (0.3, 3, 10, 20mg/L) growing hydroponically to gain insight into the morphological changes and changes in plant total protein. Our results showed remarkable changes in plant morphology and electrophoresis pattern during stress that would be very important in our further studies on molecular characterization under abiotic stress. Results indicate that Cenchrus ciliaris has the ability to grow in moderate amounts of Ni but higher concentrations are toxic and lead to death.

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Morphological characteristics and electrophoretic profiling is a primary tool to investigate Nickel (Ni) stress tolerance in Cenchrus ciliaris: A Cholistan desert grass

Abdallaha NA, Mosesb V, Prakashc C. 2014. The impact of possible climate changes on developing countries. GM Crops & Food. Biotechnology in Agriculture and the Food Chain 5, 77-80.

Ahmad MSA, Ashraf M. 2012. Essential roles and hazardous effects of nickel in plants. In Reviews of environmental contamination and toxicology. Springer New York P. 125-167.

Almeida AAFD, Valle RR, Mielke MS, Gomes FP. 2007. Tolerance and prospection of phytoremediator woody species of Cd, Pb, Cu and Cr. Brazilian Journal of Plant Physiology 19, 83-98.

Ambo-Rappe R, Lajus DL, Schreider MJ. 2011. Heavy metal impact on growth and leaf asymmetry of sea grass, Halophila ovalis. Journal of Environmental Chemistry and Ecotoxicology 3, 149-159.

Aown M, Raza S, Saleem MF, Anjum SA, Khaliq T, Wahid MA. 2012. Foliar application of potassium under water deficit conditions improved the growth and yield of wheat (Triticum aestivum L.). Journal of Animal and Plant Sciences 22, 431-437.

Arshad M, Ashraf MY, Ahmad M, Zaman F. 2007. Morpho-genetic variability potential of Cenchrus ciliaris L., from Cholistan desert, Pakistan. Pakistan Journal of Botany 39, 1481-1488.

Arshad M, Hassan AU, Ashraf MY, Noureen S, Moazzam M. 2008. Edaphic factors and distribution of vegetation in the Cholistan desert, Pakistan. Pakistan Journal of Botany 40, 1923-1931.

Ashraf MY, Sadiq R, Hussain M, Ashraf M, Ahmad MSA. 2011. Toxic Effect of Nickel (Ni) on Growth and Metabolism in Germinating Seeds of Sunflower (Helianthus annuus L.). Biological trace element research 143, 1695-1703.

Baccouch S, Chaoui A, Ferjani EE. 1998. Nickel-induced oxidative damage and antioxidant responses in Zea mays shoots. Plant Physiology and Biochemistry 36, 689-694.

Bradford MM.1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248-254.

Brahim L, Mohamed M. 2011. Effects of copper stress on antioxidative enzymes, chlorophyll and protein content in Atriplex halimus. African Journal of Biotechnology 10, 10143-10148.

Chinnusamy V, Schumaker K, Zhu J-K. 2004. Molecular genetic perspectives on cross-talk and specificity in abiotic stress signalling in plants. Journal of Experimental Botany: Crosstalk in Plant Signal Transduction Special Issue 55, 225+236.

Clemens S, Kim EJ, Neumann D, Schroeder JI. 1999. Tolerance to toxic metals by a gene family of Phytochelatin synthases from plants and yeast. The EMBO journal 18, 3325-3333.

Emamverdian A, Ding Y, Mokhberdoran F, Xie Y. 2015. Heavy Metal Stress and Some Mechanisms of Plant Defense Response. The Scientific World Journal 2015, 18.

Farooq MR, Haq MA, Akhtar J, Randhawa MA, Maqsood T. 2008. Comparative response of wheat (Triticum aestivum L.) genotypes to brackish water at seedling stage. Pakistan Journal of Agricultural Science 45, 4.

Franklin KA, Lyons K, Nagler PL, Lampkin D, Glenn EP, Molina-Freaner F, Markow T, Huete AR. 2006. Buffel grass (Pennisetum ciliare) land conversion and productivity in the plains of Sonora, Mexico. Biological Conservation 127, 62-71.

Gajewska E, Sk Å‚ odowska M, SÅ‚ aba M, Mazur J. 2006. Effect of nickel on antioxidative enzyme activities, Proline and chlorophyll contents in wheat shoots. Biologia Plantarum 50, 653-659.

Grennan AK. 2011. Metallothioneins, a Diverse Protein Family. Plant physiology 155, 1750-1751.

Griffa S, Ribotta A, Colomba ELp, Tommasino E, Carloni E, Luna C, Grunberg K . 2010. Evaluation seedling biomass and its components as selection criteria for improving salt tolerance in Buffalo grass genotypes. The Journal of the British Grassland Society 65, 358-361.

Hasanuzzaman M, Nahar K, Fujita M. 2013. Extreme temperature responses, oxidative stress and antioxidant defense in plants. Intech Open Access Publisher p.169-170.

Huang GY, Wang YS. 2010. Expression and characterization analysis of type 2 metallothionein from grey mangrove species (Avicennia marina) in response to metal stress. Aquatic Toxicology 99, 86-92.

Huillier LL, Auzac Jd, Durand M, Michaud-Ferriere N. 1996. Nickel effects on two maize (Zea mays) cultivars: growth, structure, Ni concentration, and localization. Canadian Journal of Botany 74, 1547-1554.

Hussain MB, Ali S, Azam A, Hina S, Farooq MA, Ali B, Bharwana SA, Gill MB. 2013. Morphological, physiological and biochemical responses of plants to nickel stress. African Journal of Agricultural Research 8, 1596-1602.

Jackson J. 2004. Impacts and management of Cenchrus ciliaris (buffalo grass) as an invasive species in northern Queensland. PhD thesis, James Cook University.

Jia-kuan X, Lian-xin Y, Zi-qiang W, Gui-chun D, Jian-ye H, Yu-long W. 2005. Effects of Soil Copper Concentration on Growth, Development and Yield Formation of Rice (Oryza sativa). Rice Science 12, 125-132.

Klaus A, Heribert H. 2004. Reactive oxygen species: Metabolism, Oxidative Stress, and Signal Transduction. Annual Review of Plant Biology 55, 373-399.

Kosova K, Vatamvas P, Prasil IT, Renaut J. 2011. Plant proteome changes under abiotic stress contribution of proteomics studies to understanding plant stress response. Journal of Proteomics 74, 1301-1322.

Kramer U, Talke I, Hanikenne M. 2007. Transition metal transport. FEBS Letter 581, 2263-2272.

Kumar Tewari R, Kumar P, Tewari N, Srivastava S, Sharma PN. 2004. Macronutrient deficiencies and differential antioxidant responses — influence on the activity and expression of superoxide dismutase in maize. Plant Science 166, 687-694.

Llamas A, Ullrich CI, Sanz A. 2008. Ni2+ toxicity in rice: effect on membrane functionality and plant water content. Plant Physiology and Biochemistry 46, 905-910.

Lopez MA, Magnitskiy S. 2011. Nickel: The last of the essential micronutrients. Agronomía Colombiana 29, 49-56.

Maksymiec W. 2007. Signaling responses in plants to heavy metal stress. Acta Physiologiae Plantarum 29, 177.

Maqbool MM, Ali A, Haq Tu, Majeed MN, Lee DJ. 2015. Response of spring wheat (Triticum aestivum L.) to induced water stress at critical growth stages. Sarhad Journal of Agriculture 31, 53-58.

Misra P, Nath K, Tandon PK. 2010. Effect of heavy metals (Ni and Pb) stress on sugarcane (Saccharum officinarum L.). Research in Environment and Life Sciences 3, 183-188.

Munzuroglu O, Geckil H. 2002. Effects of Metals on Seed Germination, Root Elongation and Coleoptile and Hypocotyl Growth in Triticum aestivum and Cucumis sativus. Archives of Environmental Contamination and Toxicology 43, 203-213.

Nagajyoti PC, Lee KD, Sreekanth TVM. 2010. Heavy metals, occurrence and toxicity for plants: a review. Environmental Chemistry Letters 8, 199-216.

Nagajyoti PC, Lee KD, Sreekanth TVM. 2010. Heavy metals, occurrence and toxicity for plants:. Environmental Chemistry Letters 8, 199-216.

Nouri MZ, Komatsu S. 2013. Subcellular protein overexpression to develop abiotic stress tolerant plants. Frontiers in plant science 4, 2.

Pinto E, Sigaudkutner T, Leitao MAS, Okamoto OK, Morse D, Colepicolo P. 2003. Heavy metal–induced oxidative stress in algae. Journal of Phycology 39, 1008-1018.

Prasad MNV, Malec P, Waloszek A, Bojko M, Strzałka K. 2001. Physiological responses of Lemna trisulca L. (duck weed) to cadmium and copper bioaccumulation. Plant Science 161, 881-889.

Seregin IV, Kozhevnikova AD. 2006. Physiological Role of Nickel and Its Toxic Effects on Higher Plants. Russian Journal of Plant Physiology 53.

Shah K, Kumar RG, Verma S, Dubey RS. 2001. Effect of cadmium on lipid peroxidation, superoxide anion generation and activities of antioxidant enzymes in growing rice seedlings. Plant Science 161, 1135-1144.

Sheng J, Liu K, Fan B, Yuan Y, Shen L, Ru B. 2007. Improving zinc content and antioxidant activity in transgenic tomato plants with expression of mouse metallothionein-I by Mt-I gene. Journal of Agricultural and Food chemistry 55, 9846-9849.

Solymosi K, Bertrand M. 2012. Soil metals, chloroplasts, and secure crop production. Agronomy for sustainable development 32, 245-272.

Steinberg TH, Jones LJ, Haugland RP, Singer VL. 1996. SYPRO orange and SYPRO red protein gel stains: one-step fluorescent staining of denaturing gels for detection of nanogram levels of protein. Analytical Biochemistry 239, 223-237.

Stoscheck CM. 1987. Protein assay sensitive at nanogram levels. Analytical Biochemistry 160, 301-305.

Valko M, Morris H, Cronin MTD. 2005. Metals, toxicity and oxidative stress. Current medicinal chemistry 12, 1161-1208.

Waldemar M. 2007. Signaling responses in plants to heavy metal stress. Acta Physiologiae Plantarum 29, 177-187.

Wang W, Vinocur B, Shoseyov O, Altman A. 2004. Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends in plant science 9, 244-252.

Watanabe N, Lam E. 2009. Bax inhibitor-1, a conserved cell death suppressor, is a key molecular switch downstream from a variety of biotic and abiotic stress signals in plants. International journal of molecular sciences 10, 3149-3167.

Yusuf M, Fariduddin Q, Hayat S, Ahmad A. 2011. Nickel: an overview of uptake, essentiality and toxicity in plants. Bulletin of Environmental Contamination and Toxicology 86, 1-17.

Yusuf M, Fariduddin Q, Hayat S, Hasan SA, Ahmad A. 2013. Protective response of 28-homobrassinolide in cultivars of Triticum aestivum with different levels of nickel. archives of Environmental Contamination and Toxicology 60, 68-76.

Zheng M, Tao Y, Hussain S, Jiang Q, Peng S, Huang J, Cui K, Nie L. 2015. Seed priming in dry direct-seeded rice: consequences for emergence, seedling growth and associated metabolic events under drought stress. Plant Growth Regulation 1-12.

Asma Yaqoob, Faizul Hassan Nasim, Muhammad Azhar Zia.
Morphological characteristics and electrophoretic profiling is a primary tool to investigate Nickel (Ni) stress tolerance in Cenchrus ciliaris: A Cholistan desert grass.
Int. J. Biosci. 10(6), 232-245, June 2017.
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