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Morphological and molecular characterization of three fungi strains isolated from locals foods in Burkina Faso

By: Hamidou Compaore, Hagr├ętou Sawadogo-Lingani, Larissa Yacine Ware, Filbert Guira, Serge Samandoulougou, Aly Savadogo, Alfred S. Traore

Key Words: Aspergillus, Penicillium, identification, D1-D2 region, ITS1-5.8S-ITS2 region.

Int. J. Biosci. 9(6), 374-382, December 2016.

DOI: http://dx.doi.org/10.12692/ijb/9.6.374-382

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Abstract

In this study we aims to characterize three fungi isolates S1, S2 and S3respectively collected from maize, groundnuts and rice. These isolates have antibacterial activities against two (2) Gram negative and two (2) Gram positives bacteria. Firstly identification was performed using phenotypic methods including macroscopic colony characteristics and microscopic morphology by identification key. Secondly, molecular methodwas used by two (2) PCR based on 28S ribosomal sub unit (D1-D2 region) and the hyper variable ITS1-5.8S-ITS2 region.The morphological characterization of the isolates has been confirmed by molecular characterization. Phylogenetic trees obtained allowed us to clearly identify and classify the isolates. Indeed, according to phylogenetic trees fungi isolates S1, S2 and S3 collected from local food in Burkina Faso were in the same clusters to those whose sequences are from toGenBank. As indicated by the results, isolates S1, S2 and S3 are respectively Aspergillus fumigatus, Aspergillus flavus and Penicillium citrinum.

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