International network for natural sciences – research journal
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Morphogenetic screening of pakistani spring wheat germplasm for drought tolerance

By: Najeeb Ullah Khan, Israr Ahmad, Inamullah, Ikram Muhammad, Ziaullah, Ayaz Ahmad, Dawood Khan, Murad Khan, Abdul Razzaq

Key Words: Dendrogram, DNA, Drought, PCR, Wheat.

Int. J. Biosci. 8(5), 39-44, May 2016.



Wheat (Triticum aestivum L.) is the most vital cereal crop and ranks first in world crop production. In Pakistan drought is the main environmental constraint that reduce 20% yield annually. The present study was carried out to screen fifty wheat genotypes for drought stress on the basis of morphological and molecular techniques. All the genotypes were sown in the field of Hazara University Mansehra Pakistan for three consecutive years (2013-2015). The highest Peduncle length, Spike length, Plant height, Days to 50% heading, Biological yield, Flag leaf area, Yield per plant, Spikelets per spike, 1000 grain weight and HI were observed in LYP-73(50 cm), Wardak-85(35 cm), 010724(105.6 cm), 010724(155 days), Sonalika(23 gm), soghat-90(65.4 cm/sq), 010737(13.1), Shahkar-95(72), 010748(68) and 010737(109.16) respectively. The analysis of variance (ANOVA) revealed that all parameters were found to be highly significant at (P≤0.01) level. Seven molecular markers were used for screening of drought tolerant genotypes concluded that Wafaq-2008, C-273, SA-2002 and Punjab-96 showed more resistant genes and recommended for rain fed areas of Pakistan. Furthermore, these genotypes could be used for breeding purposes to improve the crop yield against drought stress.


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