International network for natural sciences – research journal
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Molecular marker based identification of leaf rust resistance gene Lr25 in selected wheat accessions

By: Sahib Gul Afridi, Habib Ahmad, Ikram Muhammad, Maryam Mobeen, Rimsha Zainab

Key Words: Wheat accessions, Molecular, Pakistan, Leaf rust, Lr25, SSR.

Int. J. Biosci. 9(6), 266-270, December 2016.

DOI: http://dx.doi.org/10.12692/ijb/9.6.266-270

Abstract

Wheat (Triticum aestivum L.) rusts are among the most widespread and destructive diseases of wheat crop. Leaf rust, caused by Puccinia triticina Eriks is a common and widespread disease of wheat in several countries of the World including Pakistan. Molecular survey was conducted to screen altogether 19 Pakistani wheat germplasm/accessions obtained from National Agriculture Research Council (NARC) Islamabad, Pakistan. For the presence of leaf rust resistance gene Lr25, specific SSR primers linked to Lr25 were utilized. The research studies revealed that out of the 19 accessions grown in the botanical garden at Abdul Wali Khan University Mardan during the month of March and April 2013, 4 accessions were observed having Lr25 gene, which showed high resistance. Two accessions revealed moderate resistance while 13 accessions did not show the presence of Lr25 gene and were regarded as susceptible to leaf rust disease. The identification of Lr25 in Pakistani wheat germplasm will help in accelerating the breeding program in future, including pyramiding of different wheat resistant genes in wheat varieties.

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