home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Molecular characterization of wheat (Triticum aestivum L.) genotypes using ISSR marker system

Paper Details

Research Paper 01/07/2016
Views (231) Download (7)
current_issue_feature_image
publication_file

Molecular characterization of wheat (Triticum aestivum L.) genotypes using ISSR marker system

Makiya Rafiq, Rana Imtiaz Ahmed, Shoaib Liaqat, Muhammad Najeebullah, Abdul Karim, Muhammad Rafiq, Gahayour Ahmed, Muhammad Kashif Aziz
Int. J. Biosci.9( 1), 341-348, July 2016.
Certificate: IJB 2016 [Generate Certificate]
Key: DendogramGenetic similarityISSR markersWheat

Abstract

A study was conducted to examine the genetic diversity of 14 wheat (Triticum aestivum L.) genotypes, using 10 ISSR (Inter-simple sequence repeats) markers. A total of 74 alleles were produced and majority of ISSR markers showed a high level of polymorphism. The polymorphic information content (PIC) values ranged from 0.32 to 0.81 with an average of 0.56. The results showed that the number of polymorphic bands per assay unit was 6.6 for ISSR markers and the average number of the loci per assay unit was 7.4. The expected heterozygosity (Hep) obtained was 0.56. The estimates of the effective multiplex ratio (6.58) and marker index (3.29) were observed for the ISSR marker system. Therefore, ISSRs could be suitable for studying genetic diversity among wheat genotypes because of their ability to produce more number of bands. A dendogram discriminated the 14 wheat genotypes into 4 clusters. Results indicated that wheat cultivars had high genetic diversity that can be used in wheat breeding programs. More primers should be used for saturation of different regions in further studies.

VIEWS 10
Cover PDF

Al-Doss AA, Moustafa KA, Ahmed EI, Elshafei AA, Barakat MN. 2009. Assessment of genetic diversity in Saudi wheat genotypes under heat stress using molecular markers and agronomic traits. International Journal of Plant Breeding. Global Sciences Books 3,103-110.

Anonymous. 2014.  Agriculture Statistic of Pakistan, Govt. of Pakistan 2013-2014.

Barakat MN, Al-Doss AA, Moustafa KA, Ahmed EI, Elshafei AA. 2010. Morphological and molecular characterization of Saudi wheat genotypes under drought stress. Journal of Food Agriculture and Environment 8, 220-228.

Carvalho A, Lima-Brito J, Macas B, Guedes-Pinto H. 2009. Genetic diversity and variation among botanical varieties of old Portuguese wheat cultivars revealed by ISSR assays. Biochemical Genetics 47, 276-294.

Deif MH, Rashed MA, Sallom AA, Mostafa EAA, Ramadan WA. 2013. Characterization of twenty wheat varieties by ISSR markers. IDOSI Publications 10, 58-29.

Doyle JJ, Doyle JL. 1990. Isolation of plant DNA from fresh tissue. Focus 12(1), 13-15.

Finstein A, Mohammad H, Singh H. 1996. Genetic divergence in selected durum wheat genotypes of Ethiopian plasm. African Crop Science Journal 15 (2), 62-72.

Frankel OH, Bennett E. 1970. Genetic resources in plants – their exploration and conservation. International Biological Programme Handbook No. 11. Blackwell, Oxford and Edinburgh.

Fufa H, Baenziger BS, Beecher BS, Graybosch RA, Eskridge KM, Nelson LA. 2005. Genetic improvement trends in agronomic performances and end-use quality characteristics among hard red winter wheat cultivars in Nebraska. Euphytica 144, 187-198.

Fufa H, Baenziger PS, Beecher BS, Dweikat I, Graybosch RA, Eskridge KM. 2005. Comparison of phenotypic and molecular marker-based classifications of hard red winter wheat cultivars. Euphytica 145, 133-146.

Gostimsky SA, Kokaeva ZG, Konovalov FA. 2005. Studying plant genome variation using molecular markers. Russian Journal of Genetics 41, 480-492. PMID: 15909909.

Ijaz S, Khan IA. 2009. Molecular characterization of wheat germplasm using microsatellite markers. Genetics and Molecular Research 8 (3), 809-8 15.

Jin S, Meng ECH, Hu R, Rozelle S, Huang J. 2008. Contribution of wheat diversity to total factor productivity in China. Journal of Agriculture and Resource Economics 33, 449-472.

Manifesto MM, Schlatter A, Hopp HE, Suarez EY, Dubcovsky J. 2001.  Quantitative evaluation of genetic diversity in wheat germplasm using molecular markers. Crop Sciences 41, 682-690.

Maras M, Sustar-Vozlic J, Javornik B, Meglic V. 2008. The efficiency of the AFLP and SSR markers in genetic diversity estimation and gene pool classification of common bean (Phaseolus vulgaris L.). Acta Agriculturae Slovenica 91, 87-96.

Najaphy A, Parchin RA, Farshadfar B. 2012. Comparison of phenotypic and molecular characterizations of some important wheat cultivars and advanced breeding lines. Australian Journal of Crop Sciences 6, 326-332.

Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A. 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding 2, 225-238.

Prevost A, Wilkinson MJ. 1999. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and Applied genetics 98, 107-112.

Salem KFM, El-Zanaty AM, Esmail RM. 2008. Assessing wheat (Triticum aestivum L.) genetic diversity using morphological characters and microsatellite markers. World Journal of Agricultural Sciences 4, 538-544.

Sharma SN, Kumar V, Mathur S. 2009a. Comparative analysis of RAPD and ISSR markers for characterization of sesame (Sesam umindicum L.) genotypes. Journal of Plant Biochemistry and Biotechnology 18, 37-43.

Tatikonda L, Wani SP, Kannan S, Beerelli N, Sreedevi TK, Hoisington DA, Devi P, Varshney RA. 2009. AFLP- based molecular characterization of an elite germplasm collection of Jatropha curcas L., a biofuel plant. Plant Sciences 176, 505-513.

Thudi M, Bohra A, Nayak SN, Varghese N, Shah TM, Penmetsa RV, Nepolean T, Srivani G, Gaur PM, Kulwal PL, Upadhyaya HD, KaviKishor PB, Winter P, Kahl G, Town CD, Kilian A, Cook DR, Varshney RK. 2011. Novel SSR markers from BAC-end sequences, DArT arrays and a comprehensive genetic map with 1,291 marker locifor chickpea (Cicer arietinum L.). PLOS ONE 6, e27275. Doi:10.1371/journal.pone.0027275.

Submit Manuscript