International network for natural sciences – research journal
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Molecular characterization of wheat (Triticum aestivum L.) genotypes using ISSR marker system

By: Makiya Rafiq, Rana Imtiaz Ahmed, Shoaib Liaqat, Muhammad Najeebullah, Abdul Karim, Muhammad Rafiq, Gahayour Ahmed, Muhammad Kashif Aziz

Key Words: Wheat, ISSR markers, Genetic similarity, Dendogram.

Int. J. Biosci. 9(1), 341-348, July 2016.



A study was conducted to examine the genetic diversity of 14 wheat (Triticum aestivum L.) genotypes, using 10 ISSR (Inter-simple sequence repeats) markers. A total of 74 alleles were produced and majority of ISSR markers showed a high level of polymorphism. The polymorphic information content (PIC) values ranged from 0.32 to 0.81 with an average of 0.56. The results showed that the number of polymorphic bands per assay unit was 6.6 for ISSR markers and the average number of the loci per assay unit was 7.4. The expected heterozygosity (Hep) obtained was 0.56. The estimates of the effective multiplex ratio (6.58) and marker index (3.29) were observed for the ISSR marker system. Therefore, ISSRs could be suitable for studying genetic diversity among wheat genotypes because of their ability to produce more number of bands. A dendogram discriminated the 14 wheat genotypes into 4 clusters. Results indicated that wheat cultivars had high genetic diversity that can be used in wheat breeding programs. More primers should be used for saturation of different regions in further studies.


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