Isolation, screening and adaptation of bacteria isolated from sewage waste water and their ability to degrade pyrene through co-metabolism
By: Nadia Bibi
Key Words: PAHs, Solid media screening, Spray plate technique, Co-metabolism, Sewage waste water
Int. J. Biosci. 10(3), 335-349, March 2017.Certificate
Eleven bacterial wild strains were isolated from sewage waste water of PCSIR Laboratory Colony Peshawar, through serial dilution and were adapted for degradation of polycyclic aromatic hydrocarbons (PAHs). These isolates were screened for low molecular weight (LMW) and high molecular weight (HMW) PAHs. Isolated strains were screened on solid nutrient agar and then on mineral media. These bacterial isolates can bring about complete mineralization of (HMW) like pyrene. Isolates W1, W2, W8 and W11 were best on pyrene and phenanthrene respectively. PAHs compounds produced yellow coloration on mineral media plates. This yellow coloration during degradation of PAHs is the characteristic property of bacterial conversion of polyaromatic hydrocarbons to 2-hydroxylmuconic semi-aldehyde through meta-cleavage of catechol. Among these isolates, only W2 was able to grow on pyrene individually and efficiently degraded PAHs mixture. Naphthalene and acenaphthene were completely degraded in 24 hours as revealed from HPLC analysis. Catechol (64.05%) and phthalic acids (31.44%) were detected after 72 hours are the degradation products of phenanthrene and anthracene. 1-hydroxy2-naphthoic acid (63.37%) is the oxidation products of phenanthrene through naphthalene degradation pathway. Increased production of benzocaumarin (32.89-93.13%) between incubation periods of 120-168 hours can be the possible explanation that selected PAHs present in the mixture were degraded through naphthalene pathway. Experiment was performed in triplicate and data was analysed by 2-way ANOVA and level of significance < 0.002.
Isolation, screening and adaptation of bacteria isolated from sewage waste water and their ability to degrade pyrene through co-metabolism
Alias S, Talib SA, Omar M, Hussain NH. 2011. “Degradation of 3-ring PAHs by S. spiritovorum and C. urealyticum Strain under Non- Indigenous Condition,” in International Congress of Chemistry and Environment (ICCE 2011), Port Dockson Malaysia.
Arulazhagan P, Vasudevan N. 2011. Biodegradation of polycyclic aromatic hydrocarbons by a halotolerant bacterial strain Ochrobactrum sp. VA1. Marine Pollution Bulletin 62, 388–394. www.ncbi.nlm.nih.gov/pubmed
Bamforth SM, Singleton I. 2005. Rev. Bioremediation of polycyclic aromatic hydrocarbons: current knowledge and future directions. Journal of Chemical Technology and Biotechnology 80, 723–736.
Bastiaens L, Springael D, Wattiau P, Harms H, Dewachter R, Verachtert H, Diels L. 2000. Isolation of Adherent Polycyclic Aromatic Hydrocarbon (PAH)-Degrading Bacteria Using PAH-Sorbing Carriers. Applied and Environmental Microbiology 66, 1834–1843.
Chaineau CH, Morel J, Dupont J, Bury E, Oudot J. 1999. Comparison of the fuel oil biodegradation potential of hydrocarbon-assimilating microorganisms isolated from a temperate agricultural soil. Science of the Total Environment 227, 237–247.
Chaineau CH, Rougeux G, Oudot YC. 2005. Effect of nutrient concentration on the biodegradation of crude oil and associated microbial population in the soil. Journal of Soil Biology and Biochemistry 37, 1490–1497.
Clayton L. 2005. Rugh, Endang Susilawati, Alexandra N. Kravchenko, John C. Thomas Biodegrader Metabolic Expansion during Polyaromatic Hydrocarbons Rhizoremediation. Plant and Soil Science 60, 331-339.
Dabestani R, lvanov IN. 1999. A Compilation of Physical, Spectroscopic and Photophysical Properties of Polycyclic Aromatic Hydrocarbons. Photochemistry and Photobiology 70, 10-34.
Dastgheib SM, Amoozegar MA, Khajeh K, Shavandi M, Ventosa A. 2011. Biodegradation of polycyclic aromatic hydrocarbons by a halophilic microbial consortium. Applied Microbiology and Biotechnology 95, 789-798.
Ebrahimi M, Sarikhani MR, Fallah R. 2012. Assessment of biodegradation efficiency of some isolated bacteria from oil contaminated sites in solid and liquid media containing oil-compounds. International Research Journal of Applied and Basic Sciences 3, 138-147.
Fu MM, Bruce MA, Garys SN. 1993. NAH plasmid mediated catabolism of anthracene and phenanthrene to naphthoic acid. Applied Environmental Microbiology 59, 1938-1942.
www. scholar. google.com/citations
Garbeth A, Krausse S, Gemende B, Muller RH. 2004. Search for microorganisms that degrade PAH’s under alkaline conditions. Engineering and Life Sciences 4, 4.
Garcia MT, Mellado E, Ostos JC, Ventosa A. 2004. Halomonas organivorans sp. nov., a moderate halophile able to degrade aromatic compounds. International Journal of Systematic and Evolutionary Microbiology 54, 1723–1728.
Guo CL, Zhou HW, Wong YS, Tam NFY. 2005. Isolation of PAH-degrading bacteria from mangrove sediments and their biodegradation potential. Marine Pollution Bulletin 51, 1054–1061.
Haritash AK, Kaushik CP. 2009. Review on Biodegradation aspects of Polycyclic Aromatic Hydrocarbons (PAHs). Journal of Hazardous Material 169, 1–15.
Hassan EE, Hafez EE, Hussain AA, Ali AG, Hanafy AAE. 2009. Isolation and identification of three-ring polyaromatic hydrocarbons (anthracene and phenanthrene) degrading bacteria. American Eurasian Journal of Agricultural & Environmental Sciences 5, 31-38.
Herwijnen RD, Springael P, Slot H, Govers AJ, Parsons JR. 2003. Degradation of Anthracene by Mycobacterium sp. Strain LB501T Proceeds via a Novel Pathway, through o-Phthalic Acid. Applied and Environmental Microbiology 69, 186-190. www.ncbi.nlm.nih.gov
Huang L, Sturchio NC, Abrajano JT, Heraty LJ, Holt BD. 1999. Carbon and chlorine isotope fractionation of chlorinated aliphatic hydrocarbons by evaporation. Organic Geochemistry 30, 777-785. DOI: 10.1016/S0146-6380(99)00060-1
Janjua NZ, Kadir MM, Lutfi S, Tipre M, Sathiakumar N. 2013. Tasman Spirit Oil Spill in Pakistan– Research Response and Lessons Learned Dr PH. American Journal of Indian Medicine 56, 124–131.
Johnsen AR, Wick LY, Harms, H. 2005. Principles of microbial PAH-degradation in soil. Environmental Pollution 133, 71-84.
Joner EJ, Leyval C. 2003. Rhizospheric gradient of Polycyclic Aromatic Hydrocarbon (PAH) Dissipation in two industrial soils and impact of Arbuscular Mycorrhiza. Environmental Science and Technology 37, 2371-2375.
Kastner M, Breuer JM, Mahro B. 1998. Impact of inoculation protocols, salinity and pH on degradation of polycyclic aromatic hydrocarbons (PAHs) and survival of PAH-degrading bacteria introduced into soil. Applied and Environmental Microbiology 64, 359-62.
Khan K, Naeem M, Arshed MJ, Asif M. 2006. Extraction and characterization of oil degrading bacteria. Journal of Applied Sciences 6, 2302-2306. DOI: 10.3923/js.2006/2303.2306
Khan SA, Hamayun M, Bibi N, Sherwani SK. 2015. Isolation, Screening and Co-metabolism of Polycyclic Aromatic Hydrocarbons by Soil Bacteria. American Eurasian Journal of Agricultural & Environmental Sciences 15, 800-812.
Khurshid R, Sheikh MA, Iqbal S. 2008. Health of people working/living in the vicinity of an oil-polluted beach near Karachi, Pakistan. East Mediterrian Health Journal 14, 179–182. www.nap.edu/read/12949/chapter/10
Kiyohara H, Nagao K, Yana K. 1982. Rapid Screen for Bacteria Degrading Water-Insoluble, Solid Hydrocarbons on Agar Plates. Applied and Environmental Microbiology 43, 454-457. www.ncbi.nlm.nih.gov
Kobayashi H, Ritmann BE. 1982. Microbial removal of hazardous organic compounds. Environmental Science and Technology 16, 170A–183A.
Kumar A, Munjal A, Khan A, Singh RN. 2012. Mega plasmid mediated anthracene (PAH) degradation by novel strain “Geobacillus stearothermophilus AAP7919” isolated from oil contaminated soil. Journal of Pharmacy Research 5, 381-384.
Kumar G, Singla R, Kumar R. 2010. Plasmid Associated Anthracene Degradation by Pseudomonas sp. Isolated from Filling Station Site. Nature and Science 8, 89-94.
Leungsakul T, Glenn R, Johnson, Wood TK. 2006. Protein Engineering of the 4-Methyl-5-Nitrocatechol Monooxygenase from Burkholderia sp. Strain DNT for Enhanced Degradation of Nitroaromatics. Applied and Environmental Microbiology 72, 3933–3939.
DOI: 10.1128/AEM. 02966-05
Lily MK, Bahuguna A, Bhatt KK, Dangwal K. 2013. Degradation of Anthracene by a novel strain Brachybacterium paraconglomeratum BMIT637C (MTCC 9445). International Journal of Ecological Sciences 3, 1242.
Luke NU, Ugochukwu UE. 2012. The Role of pH in Nitrate-reducing Conditions in Degradation of Polycyclic Aromatic Hydrocarbons in Contaminated Soil Extract. Der Chemica Sinica 3, 435-439. http://dx.doi.org/10.5539/ijc.v5n4p43
Macleod CJA, Semple KT. 2006. The influence of single and multiple applications of pyrene on the evolution of pyrene catabolism in soil. Environmental Pollution 139, 455-460.
Maiti AK, Das S, Bhattacharyya N. 2012. Bioremediation of High Molecular Weight Polycyclic Aromatic Hydrocarbons by Bacillus thuringiensis Strain NA2. Journal of Science 72, 4.
Mallick S, Chatterjee S, Dutta TK. 2007. A novel degradation pathway in the assimilation of phenanthrene by Staphylococcus sp. strain PN/Y via meta-cleavage of 2-hydroxy-1-naphthoic acid: formation of trans-2, 3-dioxo-5-(2-hydroxyphenyl)-pent-4-enoic acid. Microbiology 153, 2104-2115.
Menzie CA, Potochi BB, Santodonato J. 1992. Exposure to carcinogenic PAHs in the environment. Environtal Science and Technology 26, 1278-1284. DOI: 10.1021/es00031a002
Moreno A, Albertelli A, Fabiano M. 2009. Nematode response to metal, PAHs and organic enrichment in Mediterranean Sea. Marine Pollution Bulletin 58, 1192-1201.
Mukesh KDJ, Sindhu G, Prasanna KP, Balakumaran MD, Kalaichelvan PT. 2012. Effect of Triton X-100 on Degradation of Polycyclic Aromatic Hydrocarbons by Pseudomonas sp. PSS6 Isolated from Municipal Wastes Sediment. Advances in Applied Science Research 3, 1383-1388. www.pelagiaresearchlibrary.com
Neelofur M, Prasad SV, Mahesh M. 2014. Enhance the Biodegradation of Anthracene by Mutation from Bacillus species. BMR Biotechnology 140, 1-19.
Obayori OS, Salam LB. 2010. Degradation of polycyclic aromatic hydrocarbons: Role of plasmids. Scientific Research and Essays Academic Journals. 5, 4093-4106.
Othman N, Hussain N, Talib SA. 2010. Degradation of polycyclic aromatic hydrocarbon by pure strain isolated from municipal sludge: Synergistic and cometabolism phenomenon. (ICENV) p. 86-90.
Park KS, Sims RC, Dupont R. 1990. Transformations of PAHs in soil systems. Journal of Environmental Engineering–ASCE 116, 632-40. http://dx.doi.org/10.1061/(ASCE)
Powell CL, Nogaro G, Agrawal A. 2011. Aerobic Cometabolic Degradation of Trichloroethene by Methane and Ammonia Oxidizing Microorganisms Naturally Associated with Carex comosa Roots. Biodegradation 22, 527-538.
Puglisi E, Cappa F, Fragolis G, Trevisan A, Del-Re, AAM. 2007. Bioavailability and degradation of phenanthrene in compost amended soils. Chemosphere 67, 548-556.
Rodrigo J, Jacques S, Santos EC, Bento FM, Maria CR, Peralba PA, Selbach ELS, Sa FAOC. 2005. Anthracene biodegradation by Pseudomonas sp. isolated from a petrochemical sludge land farming site. International Biodeterioration and Biodegradation 56, 143-150.
DOI: 10.1016/j.ibiod. 2005.06.005
Roy M, Khara P, Basu S, Dutta TK. 2013. Catabolic versatility of Sphingomonas sp. strain BNP capable of degrading structurally diverse aromatic compounds. Journal of Bioremediation and Biodegradation 4, 1-6.
Rui L, Young MK, Kenneth FR, Thomas KW. 2004. Metabolic pathway engineering to enhance aerobic degradation of chlorinated ethenes and to reduce their toxicity by cloning a novel glutathione-S-transferase, an evolved toluene o-monooxygenase, and γ-glutamylcysteinesynthetase. Environmental Microbiology 6, 491-500.
Sartoros C, Yerushalmi L, Beron P, Guiot SR. 2005. Effects of surfactant and temperature on biotransformation kinetics of anthracene and pyrene. Chemosphere 61, 1042-1050.
DOI: 10.1016/j.chemos phere.2005.02.061
Semprini L, Dolan EM, Hopkins GD, Perry LMC. 2005. Development of Effective Aerobic Cometabolic Systems for the In Situ Transformation of Problematic Chlorinated Solvent Mixtures. Strategic Environmental Research and Development Program. FINAL REPORT: ER-1127.
Shokrollahzadeh S, Golmohammad F, Shokouhi H. 2012. Study of Sphingopyxis Isolates in Degradation of Polycyclic Aromatic Hydrocarbons. Chemical Engineering Transaction 27, 55-60. www.innspub.net
Siddiqi HA, Ansari FA, Munshi AB. 2009Assessment of hydrocarbons concentration in marine fauna due to Tasman Spirit oil spill along the Clifton beach at Karachi coast. Environment Monitoring and Assessment 148, 139–148.
Simarro RN, Gonzalez L, Bautista F, Molina MC. 2013. Biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons by a wood-degrading consortium at low temperatures. FEMS Microbiology and Ecolology 83, 438–449.
Sipkema E, Marjin W, Koning JK, Ganzeveld DB, Janssen A, Beenackers ACM. 2000. NADH-Regulated Metabolic Model for Growth of Methylosinus trichosporium OB3b. Cometabolic Degradation of Trichloroethene and Optimization of Bioreactor System Performance. Biotechnology Progress 16, 189-198.
Smith PN, Cobb GP, Codding GC, Hoff D, Scott TM, Thomas RR, Kevin DR. 2007. Contaminant exposure in terrestrial vertebrates. Environmental Pollution 150, 41-64.
DOI: 10.5829/idosi.aejaes. 2015.15.5.93218
Soojhawon I, Lokhande PD, Kodam KM, Gawai KR. 2005. Biotransformation of nitroaromatics and their effects on mixed function oxidase system. Enzyme and Microbial Technology 37, 527–533.
Supakaa N, Pinphanichakarna P, Pattaragulwanita K, Thaniyavarna S, Omorib T, Juntongjin K. 2001. Isolation and Characterization of a Phenanthrene-Degrading Sphingomonas sp. Strain P2 and Its Ability to Degrade Fluoranthene and Pyrene via Cometabolism. Science Asia 27, 21-28.
Survery S, Ahmad S, Subhan SA, Ajaz M, Rasool SA. 2009. Hydrocarbon degrading bacteria from Pakistani soil: Isolation, identification, screening and genetical studies. Pakistan journal of Biological Sciences 9, 1518-1522.
www.pbs. org PJB
Tam NFY, Guo CL, Yau WY, Wong YS. 2002. Preliminary study on biodegradation of phenanthrene by bacteria isolated from mangrove sediments in Hong Kong. Marine Pollution Bulletin 45, 316–324. http://dx.doi.org/10.1016/S0025-326X(02)00108-X
Ting WTE, Yuan SY, Wu SD, Chang BV. 2011. Biodegradation of phenanthrene and pyrene by Ganoderma lucidum. International Biodeterioration and Biodegradation 65, 238-242.
Waqas M, Khan S, Chao C, Shamshad I, Qamar Z, Khan K. 2014. Quantification of PAHs and health risk via ingestion of vegetable in Khyber Pakhtunkhwa Province, Pakistan. Science of Total Environment 497, 448-458.
Ye JS, Yin H, Qianga J, Penga H, Qin MH, Zhang N, He BY. 2011. Biodegradation of anthracene by Aspergillus fumigates. Journal of Hazardous Material 185, 174–181.
Yuan SY, Wei SH, Chang BV. 2000. Biodegradation of polycyclic aromatic hydrocarbons by a mixed culture. Chemosphere 41, 1463-1468. http://dx.doi.org/10.1016/S0045-6535(99)00522-6
Zhong Y, Luan T, Wang X, Lan C, Tam NFY. 2007. Influence of growth medium on cometabolic degradation of polycyclic aromatic hydrocarbons by Sphingomonas sp. strain PheB4. Applied Microbiology and Biotechnology 75, 175–186.
Isolation, screening and adaptation of bacteria isolated from sewage waste water and their ability to degrade pyrene through co-metabolism.
Int. J. Biosci. 10(3), 335-349, March 2017.
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