International network for natural sciences – research journal
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Isolation of Clostridium perfringens from Goats and Sheep of the Khuzdar district of Balochistan, Pakistan

By: Ajaz-ul-Haq, Muhammad Kamran Taj, Imran Taj, Sana Arif, Ashfaq Ahmed, Ghulam Muhammad, Zaheer Ahmed, Zahoor Ahmed, Farhat Abbas, Abdul Samad

Key Words: Clostridium perfringens, Goats, Sheep, Khuzdar, Balochistan

Int. J. Biosci. 9(5), 156-162, November 2016.

DOI: http://dx.doi.org/10.12692/ijb/9.5.156-162

Abstract

In present study, causal agent of necrotic enteritis (NE) in goats and sheep of Khuzdar district, Balochistan was isolated. Total 200 samples were collected out of that 66.5 % were positive for Clostridium Perfringens while 33.5 % were negative. The area wise distribution of positive cases between Khuzdar 8 %, Wadh 18 %, Nall 16 %, Zehri 13.5 % and Mulla was 11%. Tehsil Wadh was highly infected in district. Whereas sheep and goats wise results showed that sheep (40.5 %) was more affected with enterotoxaemia as compared to goats (26 %). Where sex wise ratio among positive cases has been 28.5 % males (sheep 16 % and 12.5 %) and females 38 % (sheep 23 % and goats 15 %). However, age wise distributed was 8 % in one month, 18.5 % in six months, 32 % in one year, and 7.5 % in two years. C. Perfringens was confirmed through gram staining using different biochemical tests (IMVIC, Sugar fermentation, Catalase, Oxidase, Gelatin, Litmus milk and Lecithinase) and electron microscopically. Under electron microscope C. perfringens was 1.3-1 9.0µm in length and width 0.6-2.4µm. Different drugs result showed that C. Perfringens was sensitive to Chloramphenicol, Amoxycillin, Metronidazole, Vancomycin, Ciprofloxacin and Penicillin G while resistant to Polypeptides, Glycopeptides, Aminoglycosides, Cephalosporin’s, Lincosamides, Macrolides and Sulfonamides. The animal trial was carried out to check the effect of C. Perfringens in different organ. The C. Perfringens affected the intestine, liver, kidney, central nerves system and gall bladder of the mice.

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