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Isolation, identification, characterization and antibiotic susceptibility of Vibrio cholera during 1998-99.

By: Rahim Shah, Ghazala Parveen, Maria Shoukat, Sofia Khalid, Abdul Hameed

Key Words: Pathogen, Gastrointestinal disease, Seasonal outbreaks, Endemic, Antibiotic resistant strains

Int. J. Biosci. 11(3), 135-147, September 2017.


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Vibrio Cholera, causative agent of acute gastrointestinal disease or cholera is a natural inhabitant of aquatic environment. Cholera is endemic disease in Latin America, Southern Asia and parts of Africa, where poor sanitation and seasonal outbreaks are particularly associated with seasonal outbreaks. Large number of outbreaks of Vibrio cholera gastroenteritis in Asian countries indicates the need to evaluate the prevalence of that pathogenic species in different regions of Asia. This study was conducted to ascertain the prevalence and antibiotic resistance of Vibrio cholera in the endemic areas of Pakistan. Samples were collected from epidemic cell of National Institute of Health (NIH) during the time period of July 1998 to 1999, on the basis of reported cases of gastroenteritis/ cholera infections. A total of 172 isolates were collected from the 303 stools and vomitus samples of infected patients and their sensitivity to 18 antimicrobial agents were determined by disk diffusion method. All the isolates of Vibrio cholera showed 100% resistance to streptomycin and trimethoprim/ sulfamethoxazole throughout the study period. The O139 strain isolated from water was resistant to streptomycin and Kanamycin. In contrast Norfloxicin were found to be very effective with only 4% resistance rate during 1998 while Tobramycin showed the best results with only 1% resistance as compared to resistance percentage of Tetracycline 10%, Erythromycin 16%, Chloramphenicol 17%, Cefamendol 40%, Ampicillin 58%, Nalidixic acid 66%, Nitrofurantion 95% during 1999. The comparison of antibiotic sensitivity showed almost similar pattern of antibiotics sensitivity with little variations due to geographical barriers. Furthermore, the trends of increased resistance to antibiotics indicate that indiscriminate use of antimicrobial agents during hospitalization and self-medication contributed to the emergence of drug resistance in the prevalent strain of Vibrio cholera.

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Isolation, identification, characterization and antibiotic susceptibility of Vibrio cholera during 1998-99.

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Rahim Shah, Ghazala Parveen, Maria Shoukat, Sofia Khalid, Abdul Hameed.
Isolation, identification, characterization and antibiotic susceptibility of Vibrio cholera during 1998-99..
Int. J. Biosci. 11(3), 135-147, September 2017.
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