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Isolation, identification and ability of endophytic fungi in stimulating cocoa seed germination

By: Muh Assad, Muhammad Taufik, H. S. Gusnawaty, Asniah

Key Words: Cocoa, VSD disease, Endophytic fungus, Paecilomyces sp.

Int. J. Biosci. 10(1), 270-278, January 2017.

DOI: http://dx.doi.org/10.12692/ijb/10.1.270-278

Abstract

Cocoa is an important commodity of the world, with demands increasing every year. Indonesia is the third largest cocoa producer in the world and continues to increase its production. One of the factors limiting the production of cocoa is an infectious disease Vascular Streak Dieback (VSD), caused by Ceratobasidium theobromae. VSD disease can cause yield losses up to 50%, and severe infections cause the death of cocoa plants. This research was conducted using a survey method, isolation and identification of endophytic fungi based on morphological characters that were based on fungi identification book, as well as testing the ability of the fungi to stimulate germination of cacao seeds in vitro. The study was able to isolate and identify six isolates of endophytic fungi, namely Paecilomyces sp isolates EP1, EP3, EP6, and EP8, Cladosporium sp. and Nigrospora sp. Paecilomyces sp. isolate EP1 was endophytic fungus which had the ability to stimulate the germination of cacao seeds by 100%, followed by Nigrospora sp. (90%). Presumably, the endophytic fungi produced hormones to stimulate the process of cacao seed germination. One isolate of Aspergillus sp. can not be categorized as an endophytic fungus of cocoa plant. Endophytic fungus Paecilomyces sp isolate EP1 is promising to be used as an alternative environmentally friendly VSD disease control agent.

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Isolation, identification and ability of endophytic fungi in stimulating cocoa seed germination

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Muh Assad, Muhammad Taufik, H. S. Gusnawaty, Asniah.
Isolation, identification and ability of endophytic fungi in stimulating cocoa seed germination.
Int. J. Biosci. 10(1), 270-278, January 2017.
http://www.innspub.net/ijb/isolation-identification-and-ability-of-endophytic-fungi-in-stimulating-cocoa-seed-germination/
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