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Isolation, Characterization of PSB stains from rock phosphate and their potential as Biofertilizer

By: Abdul Latif, Ghulam Jilani, Rifat Hayat, Ahmed Ali Khan, Muhammad Azeem, Muhammad Ehsan, Muhammad Umair Mubarak

Key Words: PSB isolation, Characterization, Rock phosphate, Bio fertilizer

Int. J. Biosci. 10(1), 72-80, January 2017.

DOI: http://dx.doi.org/10.12692/ijb/10.1.72-80

Abstract

Rock phosphate (RP) is an important alternative source of phosphorus (P) and phosphorus solubilizing bacteria (PSB) are responsible for the efficient use of this valuable source. In vitro study was conducted in the microbiology laboratory of Soil Science PMAS-AAUR to assess the ability of PSB strains to solubilize Phosphorus from Rock Phosphate amended broth medium. For this purpose, different phosphorus solubilizing strains were first isolated from Rock Phosphate and characterized by Gram testing and API 20 E-Kit. The strains were named as Pseudomonas after characterization. These strains made halo zone around its colony, which confirmed them as P-solubilizer. Among the various P-solubilizers, best strains were picked and their efficiency was tested in the broth culture. The drop of pH and P-solubilization were the key factors to be observed. An inverse relation between drop of pH and solubilized P was noticeable. The drop of pH was due to the productions of various organic acids and was measured by pH meter while mineralization of soil P was due to acid phosphatase. The drop of pH and Phosphorus was measured by Spectrophotometer at 880 nm. The individual application of these strains showed great variation in their capability to solubilize RP and same results were seen when applied in soil. However, the co-inoculation of the strains showed significant ability to solubilize Rock Phosphate. The results of the current study confirm that PSB have great potential to be used as a bio fertilizer.

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Isolation, Characterization of PSB stains from rock phosphate and their potential as Biofertilizer

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Abdul Latif, Ghulam Jilani, Rifat Hayat, Ahmed Ali Khan, Muhammad Azeem, Muhammad Ehsan, Muhammad Umair Mubarak.
Isolation, Characterization of PSB stains from rock phosphate and their potential as Biofertilizer.
Int. J. Biosci. 10(1), 72-80, January 2017.
http://www.innspub.net/ijb/isolation-characterization-of-psb-stains-from-rock-phosphate-and-their-potential-as-biofertilizer/
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