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Isolation, characterization and control of a fungus responsible for post-harvest mango spoilage from northern region of Bangladesh

By: Md. Sifat Rahi, Israt Jerin, Salek Ahmed Sajib, Md. Shihabul Islam, Zannatul Chadni, Kazi Md. Faisal Hoque, Md Abu Reza

Key Words: Postharvest mango spoilage, ‘Totapuri’ mango, Fungus, Biological Control measurement, Citric acid.

Int. J. Biosci. 11(5), 260-269, November 2017.

DOI: http://dx.doi.org/10.12692/ijb/11.5.260-269

Certification: ijb 2017 0165 [Generate Certificate]

Abstract

Mango, ‘the king of the fruits’ is one of the most popular, nutritionally rich fruit in Bangladesh. One of the limiting factors that influence the economic value of mango is its short storage life at ambient temperature due to spoilage, caused by microbial infection. Fungi are the primary spoiling organism as they are rich in cell wall degrading enzymes which help them to spoil mango easily. Current study was carried out to isolate, characterize and to find out a cost effective control measure for the fungal strains associated with post-harvest spoilage of variety. One fungal strain was isolated from the spoiled ‘‘Totapuri’ mango. The primary characterization of the fungal strain shows that the colony diameter, culture characteristics and sporulation of the fungus is greatly influenced by the type of growth medium used. CDA exhibited higher mycelial growth, whereas the PDA culture medium was responsible for heavy sporulation. Molecular characterization involved the extraction of genomic DNA and ITS region amplification by PCR. The fungi grew optimally at 25°C, pH 9 and in dextrose carbohydrate source. The fungus exhibited high sensitivity to citric acid and NaCl, low sensitivity to plant extract of Pandanus odoratissimus and highly resistance to Borassus flabellifer, Withania sominifera, Scoparia dulci sand Allium sativum extracts. The results of this investigation combined with further studies like ITS region sequencing, can be used in precise identification of organism causing mango spoilage and it is also possible to decipher a biological control mechanism for this fungus.

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Isolation, characterization and control of a fungus responsible for post-harvest mango spoilage from northern region of Bangladesh

Agricultural Revolution. 2013.Mango Production & Technology in Bangladesh.

Agostini JP, Timmer LW. 1992. Selective isolation procedures for differentiation of two strains of Colletotrichum gloeosporioides from citrus Plant disease 76,1176-1178.

Al-Najada AR, Al-Suabeyl MS. 2014. Isolation and classification of fungi associated with spoilage of post-harvest mango (Mangifera indica L.) in Saudi Arabia African Journal of Microbiology Research 8, 685-688.

Andriani Y. 2015. Phytochemical analysis, antioxidant, antibacterial and cytotoxicity properties of keys and cores part of Pandanus tectorius fruits Arabian Journal of Chemistry.

Barnett HL, Hunter BB. 1998. Illustrated genera of imperfect fungi. vol Ed. 4. American Phytopathological Society (APS Press),

Barth M, Hankinson TR, Zhuang H, Breidt F. 2009. Microbiological spoilage of fruits and vegetables. In:  Compendium of the microbiological spoilage of foods and beverages. Springer, 135-183.

Bhatia D, Singh S, Vyas A, Rasool HI, Kaur P, Singh J. 2014. Studies on Fungal Strains of Selected Regions of Ludhiana and their Biochemical Characterization Current World Environment 9, 192-202.

Burge H. 2006. How does heat affect fungi The Environmental Reporter 4,1-3.

de Hoog GS, Guarro J. 1995. Atlas of clinical fungi. Centraalbureau voor Schimmelcultures,

Frisvad J, Samson R. 1991. Mycotoxins produced by species of Penicillium and Aspergillus occurring in cereals Developments in Food Science (Netherlands).

Hamd M, Shazia I, Iftikhar A, Fateh F, Kazmi M. 2013. Identification and characterization of post harvest fungal pathogens of mango from domestic markets of Punjab International Journal of Agronomy and Plant Production 4, 650-658.

Hibbett D. 1992. Ribosomal RNA and fungal systematics Trans Mycol Soc Jpn 33,533-556.

Horton TR, Bruns TD. 2001. The molecular revolution in ectomycorrhizal ecology: peeking into the black‐box Molecular ecology 10,1855-1871

Jamalizadeh M, Etebarian H, Aminian H, Alizadeh A. 2011. A review of mechanisms of action of biological control organisms against post‐harvest fruit spoilage EPPO Bulletin 41, 65-71.

Javadian F, Sepehri Z, Saeidi S, Hassanshahian M. 2016. Antifungal effects of the extract of the Withania somnifera on Candida albicans Advanced Herbal Medicine 2,31-37.

Kim Y, Xiao C, Rogers J. 2005. Influence of culture media and environmental factors on mycelial growth and pycnidial production of Sphaeropsis pyriputrescens Mycologia 97:25-32.

Lynd LR, Weimer PJ, Van Zyl WH, Pretorius IS (2002) Microbial cellulose utilization: fundamentals and biotechnology Microbiology and molecular biology reviews 66:506-577

Majmudar G, Modi V. 1980. Spoilage of mango by Aspergillus flavus Current Science 49, 821-822.

Mons E. 2004. Occupational asthma in greenhouse workers Current opinion in pulmonary medicine 10, 147-150.

Palejwala V, Sattur A, Modi V. 1984. Some factors responsible for the spoilage of mangoes by Penicillium cyclopium Food Microbiology 1,255-262.

Peraica M, Radic B, Lucic A, Pavlovic M. 1999. Toxic effects of mycotoxins in humans Bulletin of the World Health Organization 77,754-766.

Petzinger E, Weidenbach A.2002. Mycotoxins in the food chain: the role of ochratoxins Livestock Production Science 76,245-250.

Pitt J, Hocking A. 1997. Fungi and Food Spoilage Blackie Academic & Professional New South Wales, Australia.

Reddy VB, Madhavi GB, Reddy VC, Reddy KG, Reddy MC. 2008. Post-harvest fungal spoilage in sweet orange (Citrus sinensis) and acid lime (Citrus aurentifolia Swingla) at different stages of marketing Agr Sc Digest 28, 265-267.

Saha A, Mandal P, Dasgupta S, Saha D. 2008. Influence of culture media and environmental factors on mycelial growth and sporulation of Lasiodiplodia theobromae (Pat.) Griffon and Maubl Journal of Environmental Biology 29,407.

Sahi H. 1983. Diseases of tropical and sub-tropical fruits and their control Recent advances in plant pathology: Prof HK Saksena Festschrift/[edited by] Akhtar Husain[et al].

Saxena R, Sangeetha L, Vohra A, Gupta R, Gulati R. 2001. Induction and mass sporulation in lignin degrading fungus Ceriporiopsis subvermispora for its potential usage in pulp and paper industry Current Science 591-594.

Shams-Ghahfarokhi M. 2006. In vitro antifungal activities of Allium cepa, Allium sativum and ketoconazole against some pathogenic yeasts and dermatophytes Fitoterapia 77, 321-323.

Singh D, Sharma R. 2007. Postharvest diseases of fruit and vegetables and their management. Daya Publishing House: New Delhi, India,

Stinson EE, Osman SF, Heisler EG, Siciliano J,

Bills DD. 1981. Mycotoxin production in whole tomatoes, apples, oranges, and lemons Journal of agricultural and food chemistry 29:790-792

Tournas V, Stack ME. 2001. Production of alternariol and alternariol methyl ether by Alternaria alternata grown on fruits at various temperatures Journal of food protection 64:528-532

Vyas H, Chhatpar H. 1980. Biochemical changes in mango after infection withRhizoctonia bataticola Experientia 36, 386-387

Zheng X, Tian S, Gidley MJ, Yue H, Li B. 2007. Effects of exogenous oxalic acid on ripening and decay incidence in mango fruit during storage at room temperature Postharvest biology and technology 45, 281-284.

Md. Sifat Rahi, Israt Jerin, Salek Ahmed Sajib, Md. Shihabul Islam, Zannatul Chadni, Kazi Md. Faisal Hoque, Md Abu Reza.
Isolation, characterization and control of a fungus responsible for post-harvest mango spoilage from northern region of Bangladesh.
Int. J. Biosci. 11(5), 260-269, November 2017.
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