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An insight into recombination in CP gene of tomato infecting Chilli veinal mottle virus isolate from Pakistan

By: Adnan Ahmad, Muhammad Ashfaq, Tariq Mukhtar, Saad Imran Malik

Key Words: Solanum lycopersicum, ChiVMV, Molecular characterization.

Int. J. Biosci. 11(4), 48-54, October 2017.

DOI: http://dx.doi.org/10.12692/ijb/11.4.48-54

Certification: ijb 2017 0099 [Generate Certificate]

Abstract

Chilli veinal mottle virus (ChiVMV) is one of the significant Potyviruses infecting solanaceous crops. One of the key factors in successful survival and adoptability of an individual is genetic diversity, which is commonly influenced by recombination events. In this manuscript, molecular characterization and recombination analysis based on 3’ genomic end of a tomato infecting ChiVMV isolate (AARTPK) is presented. The 3’ genomic end of ChiVMV isolate AARTPK containing coat protein (CP) gene (459 bp) and 3’ untranslated region (390 bp) was amplified by using degenerate and specific primers in reverse transcription polymerase chain reaction (RT-PCR). In CP gene BLAST analysis, Pakistani isolate (AARTPK) shared 91-93% and 93-96% nucleotide and deduced amino acid sequence identities respectively, with other isolates of ChiVMV. In nucleotide sequence based phylogenetic analysis AARTPK clustered with Chines (YN75, YN-tobacco and Dzh-Qyg) and Italian isolate (74PAV) isolates. The information of CP gene sequence and recombination analysis generated in this research will be helpful in development of resistance tomato against ChiVMV in future.

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An insight into recombination in CP gene of tomato infecting Chilli veinal mottle virus isolate from Pakistan

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Adnan Ahmad, Muhammad Ashfaq, Tariq Mukhtar, Saad Imran Malik.
An insight into recombination in CP gene of tomato infecting Chilli veinal mottle virus isolate from Pakistan.
Int. J. Biosci. 11(4), 48-54, October 2017.
http://www.innspub.net/ijb/insight-recombination-cp-gene-tomato-infecting-chilli-veinal-mottle-virus-isolate-pakistan/
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