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Influence of water stress and Rhizobial inoculation on accumulation of flavonoids and anthocyanins in selected Common bean (Phaseolus vulgaris L.) cultivars

By: Eutropia V. Tairo, Kelvin M. Mtei, Patrick A. Ndakidemi

Key Words: Inoculants, Water, Varieties, Phytochemicals, Legumes.

Int. J. Biosci. 10(4), 333-342, April 2017.



A two season field experiment and a single season screen house experiment were conducted to assess the effect of water stress periods and rhizobial inoculation in five (5) P. vulgaris (L.) cultivars. The experiment consisted of 2 levels of rhizobia (with and without rhizobial inoculation), two stress levels (with and without water stress) and five cultivars of P. vulgaris (L.) (KAT B9, KAT B1, F9 Kidney Selection, F8 Drought line and JESCA). Results showed that flavonoid and anthocyanins (g-1.DM) concentrations were higher in non- inoculated and water stressed treatments. Varieties F8 Drought line, JESCA, and F9 Kidney Selection significantly recorded higher flavonoid and anthocyanins content in both field and screen house experiment as compared with the other tested varieties. Significant interactive effects were also observed between inoculation, water stress periods and the tested P vulgaris (L.) varieties.

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Influence of water stress and Rhizobial inoculation on accumulation of flavonoids and anthocyanins in selected Common bean (Phaseolus vulgaris L.) cultivars

Agati G, Azzarello E, Pollastri S, Tattini M. 2012. Flavonoids as antioxidants in plants: location and functional significance. Plant Science 196, 67-76.

Agbicodo EM, Fatokun CA, Muranaka RGF, Visser RGF, Linden van der CG. 2009. Breeding drought tolerant cowpea; constraints, accomplishments, and future prospects. Euphytica 167, 353-370.

Awad MA, Jager A. 2002. Relationship between fruit nutrients and concentrations of flavonoids and chlorogenic acid in ‘Elstar’ apple skin. Scientia Horticulturae 92, 265-276.

Ayoola OT. 2010. Yield Performance of Crops and Soil Chemical Changes under Fertilizer Treatments in a Mixed Cropping System. African Journal of Biotechnology 9(26), 4018-4021.

Balakumar T, Hani Babu Vincent V, Paliwal K. 1993. On the interaction of UV-B radiation (280-3 15nm) with water stress in crop plants. Physiologia Plantarum 87, 217-222.

Barnabas B, Jager K, Feher A. 2008. The effect of drought and heat stress on reproductive processes in cereals. Plant cell Environment 31, 11-38.

Bergman W. 1992. Nutritional disorders of plants development, visual and analytical diagnosis, Gustav Fischer Vela Jena, Stuttgart, New York.

Bongue-Bartelsman M, Phillips DA. 1995. Nitrogen stress regulates gene expression of enzymes in the flavonoid biosynthetic pathway of tomato plant. Physiology and Biochemistry Paris 33, 539-546.

Chalker-Scott L. 1999. Environmental Significance of Anthocyanins in Plant Stress Responses. Photochemistry and Photobiology 70(1), 1-9.

Di Ferdinando M, Brunetti C, Agati G, Tattini M. 2014. Multiple functions of polyphenols in plants inhabiting unfavourable Mediterranean areas. Environmental and Experimental Botany 103, 107-116.

Di Ferdinando M, Brunetti C, Fini A, Tattini M. 2012. Flavonoids as antioxidants in plants under abiotic stresses. In: Ahmad P, Prasad MNV, editors. Abiotic Stress Responses in Plants: Metabolism Productivity and Sustainability. New York: Springer Verlag pp. 159-179.

Dixon RA, Steele C. 1999. Flavonoids and isoflavonoids-gold mine for metabolic engineering. Tropical Plant Science 4, 394-400.

Falcone Ferreyra ML, Rius SP, Casati P. 2012. Flavonoids: biosynthesis, biological functions, and biotechnological applications. Frontiers in Plant Science 3, 222.

Farooq M, Basra SMA, Wahid A, Cheema ZA, Cheema MA, Khaliq A. 2008. Physiological role of exogenously applied glycinebetaine in improving drought tolerance of fine grain aromatic rice (Oryza sativa L.). Journal Agronomy Crop Science 194, 325-333.

Farooq M, Wahid A, Kobayashi N, Fujita D, Basra SMA. 2009. Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development 29, 185-212.

Fini A, Brunetti C, Di Ferdinando M, Ferrini F, Tattini M. 2011. Stress-induced flavonoid biosynthesis and the antioxidant machinery of plants. Plant Signaling & Behavior 6(5), 709-711.

DOI: 10.4161/psb.6.5.15069.

Horbowicz M, Kosson R, Grzesiuk A, Dębski H. 2008. Anthocyanins in fruit and vegetables – their occurrence, analysis and role in human nutrition. Vegetable Crops Research Bulletin 68, 5-22.

Ibrahim MH, Jaafar HZE, Rahmat A, Rahman ZA. 2011. The Relationship between Phenolics and Flavonoids Production with Total Non Structural Carbohydrate and Photosynthetic Rate in Labisia pumila Benth. under High CO2 and Nitrogen Fertilization. Molecules 16, 162-174.

DOI :10.3390/ molecules16010162.

Kondorosi A, Ratet P, Coronado C. 1995. Alfalfa root flavonoid production is nitrogen regulated. Plant Physiology 108, 533-542.

Larson RA. 1988. The antioxidants of higher plants. Phytochemistry 27, 969-78.

Laurentius H, Nugroho, Marianne C. 2002. Activities of enzymes involved in the phenylpropanoid pathway in constitutively salicylic acid-producing tobacco plants. Plant Physiology and Biochemistry 40, 755-760.

Lindoo SJ, Caldwell MM. 1978. Ultraviolet-B radiation induced inhibition of leaf expansion and promotion of anthocyanin production. Lack of the involvement of the low irradiation phytochrome system. Plant Physiology 61, 278-282.

Liu W, Zhu DW, Liu DH, Geng MJ, Zhou WB, Mi WJ, Yang TW, Hamilton D. 2010. Influence of nitrogen on the primary and secondary metabolism and synthesis of flavonoids in Chrysanthemum morifolium, ramat. Journal of Plant Nutrition 33(2), 240-254.

DOI: 10.1080/01904160903434287.

Makoi JHJR, Bambara S, Ndakidemi PA. 2010. Rhizosphere phosphatase enzyme activities and secondary metabolites in plants as affected by the supply of Rhizobium, lime and molybdenum in Phaseolus vulgaris L. Australian Journal of Crop Sciences 4(8), 590-597.

Makoi JHJR, Belane A, Chimphango SMB, Dakora FD. 2010b. Flavonoids and anthocyanins in seed exudates enhance nodulation and defense in symbiotic cowpea (Vigna unguiculata L. Walp.). Field Crops Research 118, 21-27.

Mazid M, Khan TA, Mohammad F. 2011. Role of Secondary metabolites in defense mechanisms of plants. Biology and Medicine 3(2), 232-249.

Mierziak J, Kostyn K, Kulma A. 2014. Flavonoids as Important Molecules of Plant Interactions with the Environment. Molecules 19, 16240-16265.

Mirecki RM, Teramura AH. 1984. Effects of UV-B irradiance on soybean. V. The dependence of plant sensitivity on the photosynthetic photon flux density during and after leaf expansion. Plant Physiology 74, 475-480.

Ndakidemi PA, Dakora FD. 2003. Legume seed flavonoids and nitrogenous metabolites as signals and protectants in early seedling development. Functional Plant Biology 30, 729 -745.

Ndakidemi PA, Makoi JHR. 2009. Effect NaCl on the productivity of four selected bean cultivars (Phaseolus vulgaris L.). Scientific Research and Essays 4(10), 1066-1072.

Odjegb VJ, Alokolaro AA. 2013. Stimulated Drought and Salinity Modulates the Production of Phytochemicals in Acalypha wilkesiana. Journal of Plant Studies 2(2), 105-112.

Ramachandra RS, Ravishankar GA. 2002. Plant cell cultures: chemical factories of secondary metabolites. Biotechnology Advances 20, 101-153.

Ramakrishna A, Ravishankar A. 2011. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signaling & Behavior 6(11), 1720-1731.

Razal RA, Ellis S, Singh S. 1996. Nitrogen recycling in phenylpropanoid metabolism. Phytochemistry 41, 31-35.

Siddiqui MH, Al-Khaishany MY, Al-Qutami MA, Al-Whaibi MH, Grover A, Ali HM, Al-Wahibi MS, Bukhari NA. 2015. Response of Different Genotypes of Faba Bean Plant to Drought Stress. International Journal of Molecular Sciences 16, 10214-10227.

Steel RGD, Torrie JH. 1980. Principles and Procedures of Statistics: A Biometrical Approach, 2nd Edition, McGraw Hill, New York.

Stewart AJ, Chapman W, Jenkins GI. 2001. The effect of nitrogen and phosphorus deficiency on flavonol accumulation in plant tissue. Plant Cell and Environment 24, 1189-1197.

Tairo EV, Mtei KM, Ndakidemi, PA. 2017. Influence of Water Stress and Rhizobial Inoculation on Accumulation of Proline in Selected Cultivars of Phaseolus vulgaris (L.). International Journal of Current Microbiology and Applied Sciences 6(3), 2205-2214.

Zadehbagheri M. 2014. The evaluation of various agronomic traits of Red & Chiti bean genotypes under well-watered and drought stress conditions. International Journal of Farming and Allied Sciences 3(4), 389-398.

Eutropia V. Tairo, Kelvin M. Mtei, Patrick A. Ndakidemi. 2017. Influence of water stress and Rhizobial inoculation on accumulation of flavonoids and anthocyanins in selected Common bean (Phaseolus vulgaris L.) cultivars. Int. J. Biosci. 10(4), 333-342.
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