International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Influence of dietary lipid and Vitamin E levels on lipid peroxidation of Labeo rohita fingerlings

By: Mahroze Fatima, Muhammad Afzal, Syed Zakir Hussain Shah, Saadia Tabassum

Key Words: α-tocopherol acetate, SOD, Catalase, Peroxidase, TBARS

Int. J. Biosci. 10(4), 113-120, April 2017.

DOI: http://dx.doi.org/10.12692/ijb/10.4.113-120

Certificate
Cover

Abstract

Increased dietary lipids provide more energy and highly unsaturated fatty acids to fish. However, the fish having high lipid concentrations is at more risk of lipid peroxidation, which is counteracted vitamin E as an antioxidant. A 2×3 factorial experiment under completely randomized design was executed to study the influence of increased lipid and vitamin E levels on peroxidation status of L. rohita fingerlings. The status of lipid peroxidation was determined by studying the level of thiobarbituric acid reactive substances (TBARS) and antioxidant enzyme activities. Six experimental diets were made containing two levels of lipids (8 and 16%) and three levels of vitamin E (0, 100, 1000 mg/kg). Upon onset of feeding trial, twenty-five fish were stocked randomly in triplicates against each dietary treatment and fed twice a day. Upon termination of 60 days feeding trial, fish were sacrificed and analyzed for TBARS and antioxidant enzyme activities. Increased supplementation of dietary lipids, increase the TBARS contents in fish body, which were significantly decreased by supplementation of vitamin E. Similarly, increased dietary lipids also increased the activities of superoxide dismutase (SOD), catalase and peroxidase in the whole-body of fish. Supplementation of dietary vitamin E significantly reduced the activities of these enzymes. Moreover, lower values of TBARS and antioxidant enzyme activities were recorded with adequate levels (100 mg/kg) of vitamin E supplementation at 8% lipid level while high dose (1000 mg/kg) of vitamin E showed reduced rate of lipid peroxidation at 16% lipid level. Conclusively, this study suggested an increased requirement of dietary vitamin E to cope the oxidative stress caused by an increased dietary lipids level.

| Views 28 |

Influence of dietary lipid and Vitamin E levels on lipid peroxidation of Labeo rohita fingerlings

AOAC (Association of Official Analytical Chemists). 1995. Official Methods of Analysis. 15th Ed., Association of Official Analytical Chemist, Washington, D.C. USA, 1094 P.

Bai SC, Gatlin DM. 1993. Dietary vitamin-E

concentration and duration of feeding affect tissue alpha-tocopherol concentrations of channel catfish (Ictalurus punctatus). Aquaculture 113, 129- 135.

http://dx.doi.org/10.1016/0044-8486(93)90346-Z.

Chaiyapechara S, Casten MT, Hardy RW, Dong FM. 2003. Fish performance, fillet characteristics, and health assessment index of rainbow trout (Oncorhynchus mykiss) fed diets containing adequate and high concentrations of lipid and vitamin E. Aquaculture 219, 715-738.

http://dx.doi.org/10.1016/S0044-8486(03)00025-5.

Chance B, Maehly AC. 1955. Assay of catalase and peroxidases. Methods in Enzymology 2, 764-775. http://dx.doi.org/10.1016/S0076-6879(55)02300-8.

Cho CY, Kaushik SJ. 1990. Nutritional energetics in fish: energy and protein utilization in rainbow trout (Salmo gairdneri). In: Bourne GH, Ed. Aspects of food production, consumption and energy values. Karger Publishers, 132-172.

http://dx.doi.org/10.1159/000417529.

Civello PM, Marting GA, Chaves AR, Anan MC. 1995. Peroxidase from strawberry fruit by partial purification and determination of some properties. Journal of Agricultural and Food Chemistry 43, 2596-2601.

http://dx.doi.org/10.1021/jf00058a008.

Gatta PP, Pirini M, Testi S, Vignola G, Monetti PG. 2000. The influence of different levels of dietary vitamin E on sea bass Dicentrarchus labrax flesh quality. Aquaculture Nutrition 6, 47-52.

http://dx.doi.org/10.1046/j.13652095.2000.00127.x.

Giannopolitis CN, Ries SK. 1977. Superoxide dismutase І occurrence in higher plants. Plant Physiology 59, 309-314.

http:/ / dx. doi. org/ 10. 1104/ pp. 59. 2. 309

Hardy RW. 1999. Problems and opportunities in fish feed formulation. Aquaculture Magazine-Arkansas 25, 56-60.

Huang CH, Huang SL. 2004. Effect of dietary vitamin E on growth, tissue lipid peroxidation, and liver glutathione level of juvenile hybrid tilapia, Oreochromis niloticus×O. aureus fed oxidized oil. Aquaculture 237, 381-389.

http://dx.doi.org/10.1016/j.aquaculture.2004.04.002

Lewis-McCrea LM, Lall SP. 2007. Effects of moderately oxidized dietary lipid and the role of vitamin E on the development of skeletal abnormalities in juvenile Atlantic halibut (Hippoglossus hippoglossus). Aquaculture 262, 142-155. http://dx.doi.org/10.1016/j.aquaculture.2006.09.024

Li P,Gatlin III DM. 2009. Dietary vitamin E requirement of the red drum Sciaenops ocellatus. Aquaculture Nutrition15, 313-319.

http://dx.doi.org/10.1111/j.1365-2095.2008.00596.x.

Lin MF, Shiau SY. 2005. Dietary vitamin E requirement of grouper, Epinephelus malabaricus at two lipid levels, and their effects on immune responses. Aquaculture 248, 235-244.

http://dx.doi.org/10.1016/j.aquaculture.2005.04.020

Mourente G, Diaz-Salvago E, Bell JG, Tocher DR. 2002. Increased activities of hepatic antioxidant defence enzymes in juvenile gilthead sea bream (Sparus aurata L., Osteichthyes, Sparidae) fed dietary oxidised oil: attenuation by dietary vitamin E. Aquaculture 214, 343-361.

http://dx.doi.org/10.1016/S0044-8486(02)00064-9.

Peng S, Chen L, Qin JG, Hou J, Yu N, Long Z, Li E, Ye J. 2009. Effects of dietary vitamin E supplementation on growth performance, lipid peroxidation and tissue fatty acid composition of black sea bream (Acanthopagrus schlegeli) fed oxidized fish oil. Aquaculture Nutrition 15, 329-337. http://dx.doi.org/10.1111/j.1365-2095.2009.00657.x

Puangkaew J, Kiron V, Somamoto T, Okamoto N, Satoh S, Takeuchi T, Watanabe T. 2004. Nonspecific immune response of rainbow trout (Oncorhynchus mykiss Walbaum) in relation to different status of vitamin E and highly unsaturated fatty acids. Fish and Shellfish Immunology 16, 25-39. http://dx.doi.org/10.1016/S1050-4648(03)00028-7.

Ruff N, Fitzgerald RD, Cross TF, Kerry JP. 2002. Fillet shelf-life of Atlantic halibut Hippoglossus hippoglossus L. fed elevated levels of alpha-tocopheryl acetate. Aquaculture Research33, 1059-1071.

http://dx.doi.org/10.1046/j.13652109.2002.00770.x.

Sargent JR, Bell JG, McEvoy L, Tocher DR, Estevez A. 1999. Recent developments in the essential fatty acid nutrition of fish. Aquaculture 177, 191-199.

http://dx.doi.org/10.1016/S0044-8486(99)00083-6.

Sau SK, Paul BN, Mohanta KN, Mohanty SN. 2004. Dietary vitamin E requirement, fish performance and carcass composition of rohu, Labeo rohita fry. Aquaculture 240, 359-368.

http://dx.doi.org/10.1016/j.aquaculture.2004.02.008

Schwarz FJ, Kirchgessuner M, Steinhart H, Runge G. 1988. Influence of different fats with varying additions of a-tocopherol acetate on growth and body composition of carp (Cyprinus carpio L.). Aquaculture 69, 57-67.

http://dx.doi.org/10.1016/0044-8486(88)90186-X.

Shiau SY, Shiau LF. 2001. Re-evaluation of the vitamin E requirements of juvenile tilapia (Oreochromis niloticus×O. aureus). Animal Science 72, 529-534.

https://doi.org/10.1017/S135772980005205X

Tocher DR, Ghioni C. 1999. Fatty acid metabolism in marine fish: low activity of fatty acyl Δ5 desaturation in gilthead sea bream (Sparus auratus) cells. Lipids 34, 433-440.

http://dx.doi.org/10.1007/s11745-999-0382-8.

Tocher DR, Mourente G, Van der Eecken A, Evjemo JO, Diaz E, Bell JG, Geurden I, Lavens P, Olsen Y. 2002. Effects of dietary vitamin E on antioxidant defence mechanisms of juvenile turbot (Scophthalmus maximus L.), halibut (Hippoglossus hippoglossus L.) and sea bream (Sparus aurata L.). Aquaculture Nutrition 8, 195-207.

http://dx.doi.org/10.1046/j.13652095.2002.00205.x.

Wang J, Xu Y, Li X, Li J, Bao P, Che J, Li S, Jin L. 2015. Vitamin E requirement of sea cucumber (Apostichopus japonicus) and its’ effects on nonspecific immune responses. Aquaculture Research 46, 1628-1637.

http://dx.doi.org/10.1111/are.12324.

Watanabe T. 1982. Lipid nutrition in fish. Comparative Biochemistry and Physiology 73B, 3-15.

http://dx.doi.org/10.1016/0305-0491(82)90196-1.

Zhou QC, Wang LG, Wang HL, Wang T, Elmada CZ, Xie FJ. 2013. Dietary Vitamin E could improve growth performance, lipid peroxidation and non-specific immune responses for juvenile cobia (Rachycentron canadum). Aquaculture Nutrition 19, 421-429.

http://dx.doi.org/10.1111/j.1365-2095.2012.00977.x.

Mahroze Fatima, Muhammad Afzal, Syed Zakir Hussain Shah, Saadia Tabassum.
Influence of dietary lipid and Vitamin E levels on lipid peroxidation of Labeo rohita fingerlings.
Int. J. Biosci. 10(4), 113-120, April 2017.
http://www.innspub.net/ijb/influence-dietary-lipid-vitamin-e-levels-lipid-peroxidation-labeo-rohita-fingerlings/
Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
http://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update