International network for natural sciences – research journal
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Incidence of multidrug resistant coagulase negative Staphylococci in clinical samples of Tertiary care hospitals of Khairpur and Sukkur cities of Pakistan

By: Shahida Mangi, Anwar Hussain Phulpoto, Muneer Ahmed Qazi, Nisar Ahmed Kanhar

Key Words: Coagulase-negative staphylococci, Multidrug resistance, Antimicrobial sensitivity profiling, Clinical samples, Tertiary care hospitals

Int. J. Biosci. 9(4), 261-269, October 2016.



Coagulase-negative staphylococci (CoNS) adopting multidrug resistance have been increasingly becoming a threat to the patients in tertiary care hospitals worldwide. The aim of present study was to find out the incidence of multidrug-resistant CoNS in various samples of clinical origin. A total of 280 clinical samples of different origin were collected from tertiary care hospitals of Khairpur and Sukkur cities of Pakistan. Coagulase-negative staphylococci (CoNS) were isolated and identified using routine microbiological techniques and molecular characterization using 16S rRNA sequence-based homology. The antimicrobial sensitivity was determined using Kirby-Bauer’s disc-diffusion assay and penicllin zone-edge test. Overall, 44 (21.15%) samples were found positive for Gram positive staphylococci, i.e. 35(80%) coagulase-positive and 9(20%) coagulase-negative. The highest percent prevalence of CoNS was found in urine samples 2/6(33%) and ear swab 1/3(33%) followed by throat swab 1/4(25%) and pus 5/27(19.1%). The results of antibiotic sensitivity profiling revealed complete resistance of the pus isolate CoNS5 to all the antibiotics tested. The phylogenetic correlation of amplified 16S rRNA gene sequence of CoNS5 isolate shared 98% similarity with Staphylococcus haemolyticus ATCC 29970 (GenBank accession no. D83367). Prevalence of extended multidrug-resistant pathogen S. haemolyticusin clinical specimenscalls for timely control measures against next superbug after methicillin-resistant S. aureus (MRSA).

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