In vivo, in vitro antioxidant and hypolipidemic activity of methanol extract of Pineapple peels in Wistar Rats
By: Ejiofor U. Emmanuel, Ebhohon S. Onagbonfeoana, Obike C. Adanma, Obinwanne C. Precious, Azubike-Izah Faith, Omeh Y. Ndukaku
Key Words: HDL, Pineapple peels, DPPH, Cholesterol, MDA.
Int. J. Biosci. 8(6), 64-72, June 2016.
The medicinal values of pineapple has been well documented in literature. However, pineapple peels constitute the major waste of the pineapple fruit. Recent interest has been on harnessing plants waste materials for economic and industrial values. The study investigated the in vivo, in vitro and hypolipidaemic activity of pineapple peel extract on wistar albino rats. Twenty wistar albino rats grouped into four groups were used for the study. Group A served as control group, while group C-D received varying doses of the crude extract (200, 300 and 500mg/kg body weight). Experimental period lasted for twenty eight days, after which animals were fasted overnight and blood collected via ocular puncture for lipid profile analysis and liver obtained for antioxidant analysis. The in vitro antioxidant activity was determined using 2, 2- diphenyl-1-picrylhydrazine photometric assay and ferric reducing ability assay. Result indicated a concentration dependent (25- 400 µg/mL) for the extract in 2, 2- diphenyl-1-picrylhydrazine photometric assay and ferric reducing ability assay. In vivo antioxidant assay showed no significant (P<0.05) difference in the extract administered group when compared to the control group. Serum HDL estimation showed a significant (P<0.05) increase in the extract administered group (200 and 500mg/kg) when compared to the control group. Result for in vivo showed that the extract did not induce oxidative stress and can be a positive agent in preventing lipid peroxidation while increase in functional HDL indicated that pineapple peels may be useful in the management of hyperlipidaemia and other associated diseases.