International network for natural sciences – research journal
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In vitro antibacterial activity of Fumaria indica (H.) pugsley and SilybummarianumL. against planktonic and biofilm form of Pseudomonas aeruginosa

By: Imran Khan, Azhar Hussain Shah, Wajiha Khan, Uzma Khan

Key Words: Antibacterial activity, Fumaria indica, Silybummarianum, Pseudomonas aeruginosa, Biofilm, Planktonic.

Int. J. Biosci. 8(4), 94-102, April 2016.



Present study investigated antibacterial potential of aerial partsof Fumaria indica andSilybummarianum against planktonic and biofilm form of 3 clinical strains P1, P2 and P3 of Pseudomonas aeruginosa. Antibacterial activity against planktonic form was investigated by Well diffusion method. The antibiofilm activity was assessed by using Pellicle inhibition (PI) and Congo red assay (CR). Crude methanolic extracts of Fumaria indicashowed good antibacterial activity against P. aeruginosa with maximum 15.8mm zone of inhibition (ZOI) against P2 strain of P. aeruginosa and minimum 13.2mm zone of inhibition against P3 strain. Silybummarianumshowed maximum 13.3mm zone of inhibition and minimum 12.6mm ZOI against P2 and P3 strains of P. aeruginosa respectively.Furthermore, F. indica showed a moderate (+++) to weak (++++) antibiofilm activity against all tested strains of P. aeruginosa in Pellicle inhibition assay, while S. marianum possessed from moderate to no activity against tested bacterial strains, respectively. In Congo red assay F. indica showed a strong antibiofilm effect as compared to S. marianum. Based on these results it may be concluded that leaf extractof Fumaria indicapossessed a good to moderate antibacterial activityagainst planktonic and biofilm form of P. aeruginosa. While Silybummarianumpossessed moderate to weak antibacterial potential against P. aeruginosa.


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